Microbial Transformations of Steroids

Chemical and biochemical approaches to the production of 7-hydroxylated C19-steroids

“…The use of yeast is to be preferred over the use of E. coli strains, which cannot process the translation-resulting protein. Many enzymatic reactions specifi c to steroids are carried out by fungi and have been reported [29] . Use of mycelium broth has generally been applied, and the specifi c enzymes involved have not been fully studied since.…”

L'oxydation de la prégnènolone en progestérone par des préparations de tissus des Orthoptères in vitro

“…Les syst6mes enzymatiques CtudiCs dans ce travail ont dCj& Ct C t~ouvCs chez plusieurs chordCs (30,32), certains microorganismes (321, et certaioes plantes (33). Ces syst6mes ont aussi Ct C dCcelCs chez les invertCbrCs soit l'huitre (11) et la limace (34) ; cependant, c'est la premi6re fois que leur prCsence est dCcelCe dam les tissus d 'insectes.…”

Thin-Layer Chromatography of Some C21, C19 and C18 Steroids

“…The aim of the present work is to report on a convenient collection of solvent systems and colour reagents for the resolution and identification of different C18, C19 and C21 steroids. The majority of these compounds were recorded to be transformation products formed by various active strains acting on different steroid substrates (PETERSON (7) and CAPEK et al (8)). It is hoped that this work will participate in dissolving the problem of identification of some transformation products encountered by the authors concerned themselves in studying the microbiological transformation of steroids.…”