Microbial structures and community functions of anaerobic sludge in six full-scale wastewater treatment plants as revealed by 454 high-throughput pyrosequencing

Shu, Duntao; He, Ya‐Ling; Yue, Hong; Wang, Qingyi

doi:10.1016/j.biortech.2015.03.072

Cited by 278 publications

(81 citation statements)

References 36 publications

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“…Similar to the results of this study, the dominance of Betaproteobacteria (higher taxa) has been frequently observed in phenol degradation systems, including CWW (Manefield et al 2005;Zhu et al 2013Zhu et al , 2016Ma et al 2015a;Wang et al 2015b) petroleum refinery, pharmaceutical (Ibarbalz et al 2013), and municipal Wang et al 2012;Hai et al 2014;Shu et al 2015) wastewater treatment plants, as well as in different types of bioreactors (Wagner and Loy 2002). This highlights the rapid a c c l i m a t i z a t i o n a n d m e t a b o l i c s i g n i f i c a n c e o f Betaproteobacteria in the removal of a wide range of pollutants, including phenolic compounds from CWW.…”

Section: Discussionsupporting

confidence: 89%

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Joshi

Zhang

Tian

et al. 2016

Appl Microbiol Biotechnol

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The combined anaerobic-aerobic biosystem is assumed to consume less energy for the treatment of high strength industrial wastewater. In this study, pollutant removal performance and microbial diversity were assessed in a longterm (over 300 days) bench-scale sequential anaerobicaerobic bioreactor treating coking wastewater. Anaerobic treatment removed one third of the chemical oxygen demand (COD) and more than half of the phenols with hydraulic retention time (HRT) of 42 h, while the combined system with total HRT of 114 h removed 81.8, 85.6, 99.9, 98.2, and 85.4 % of COD, total organic carbon (TOC), total phenols, thiocyanate, and cyanide, respectively. Two-dimensional gas chromatography with time-of-flight mass spectrometry showed complete removal of phenol derivatives and nitrogenous heterocyclic compounds (NHCs) via the combined system, with the anaerobic process alone contributing 58.4 and 58.6 % removal on average, respectively. Microbial activity in the bioreactors was examined by 454 pyrosequencing of the bacterial, archaeal, and fungal communities. Proteobacteria (61.2-93.4 %), particularly Betaproteobacteria (34.4-70.1 %), was the dominant bacterial group. Ottowia (14.1-46.7 %), Soehngenia (3.0-8.2 %), and Corynebacterium (0.9-12.0 %), which are comprised of phenol-degrading and hydrolytic bacteria, were the most abundant genera in the anaerobic sludge, whereas Thiobacillus (6.6-43.6 %), Diaphorobacter (5.1-13.0 %), and Comamonas (0.2-11.1 %) were the major degraders of phenol, thiocyanate, and NHCs in the aerobic sludge. Despite the low density of fungi, phenol degrading oleaginous yeast Trichosporon was abundant in the aerobic sludge. This study demonstrated the feasibility and optimization of less energy intensive treatment and the potential association between abundant bacterial groups and biodegradation of key pollutants in coking wastewater.

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Section: Discussionsupporting

confidence: 89%

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Joshi

Zhang

Tian

et al. 2016

Appl Microbiol Biotechnol

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“…Even though present in large amounts in the CI, the percentage of bacteria from the Nitrospirae phylum were significantly reduced in the RBC; the relatively low abundance of these bacteria in RAS biofilters has been reported previously (Ruan et al, 2015; Huang et al, 2016). The Planctomycetes were numerous in the CI and RBC and they have been found in different freshwater (Sugita et al, 2005; van Kessel et al, 2010) and marine (Tal et al, 2003; Michaud, 2007; Interdonato, 2012; Ruan et al, 2015; Huang et al, 2016) nitrification filters, marine RAS (Cytryn et al, 2003) and sludge of wastewater treatment plants (Shu et al, 2015). …”

Section: Discussionmentioning

confidence: 99%

“…Acenitobacter have also been identified from the Mediterranean (Cavallo et al, 2009), sea bass reared in northern Adriatic Sea (Čož-Rakovac et al, 2002), coke plant wastewater (Liu et al, 2015), freshwater (Itoi et al, 2007; Schneider et al, 2007; Schreier et al, 2010) and marine aquaculture systems (Michaud, 2007). Representatives of the genus Halorhodospira perform oxidation of sulfides (Imhoff and Süling, 1996) and their relatives have been found in the wastewater treatment systems (Shu et al, 2015) and in marine RAS (Interdonato, 2012). …”

Section: Discussionmentioning

confidence: 99%

“…It is conceivable that the RBC dominating Flavobacteriales were aerobic bacteria that use inorganic nitrogen, while the ones inhabiting denitrification reactors were anaerobes able to use organic compounds from the wort. This group of bacteria has been found in aeration basin (Preut, 2014) and anaerobic sludge (Shu et al, 2015) of different full-scale wastewater treatment plants as well as the Eastern Mediterranean water column (Techtmann et al, 2015). …”

Section: Discussionmentioning

confidence: 99%

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Bacterial community analysis of marine recirculating aquaculture system bioreactors for complete nitrogen removal established from a commercial inoculum

et al. 2019

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An experimental recirculating aquaculture system was constructed under ambient seawater conditions to compare microbial community diversity of nitrifying and denitrifying biofilters that were derived from a commercial inoculum used for aquarium applications. Next generation sequencing revealed distinct and diverse microbial communities in samples analyzed from the commercial inoculant and the denitrification and nitrification biofilters. In all samples, communities were represented by a few dominant operational taxonomic units (OTUs). Bacteria having the capacity to carry out ammonia and nitrite oxidation were more abundant in the nitrification biofilter. Similarly, the proportion of the bacterial taxa known to carry out heterotrophic and autotrophic denitrification and participate in sulfur cycling were found in the denitrification bioreactor, and likely originated from the ambient environmental water source. Our results indicated that environmental seawater can be a favorable enhancement to the bacterial consortium of recirculating aquaculture systems biofilters.

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“…Despite their widespread use to lower N inputs to sensitive ecosystems and to protect drinking water resources for nearly three decades (Oakley et al, 2010), there is little information on the structure of microbial communities that carry out N transformation in advanced OWTS. This is in contrast with centralized WTPs, where information on the size, structure, and function of relevant microbial communities is used to understand and improve treatment (Kim et al, 2013; Jaranowska et al, 2013; Lu et al, 2014; Wang et al, 2014; Shu et al, 2015) and lower greenhouse gas emissions (Song et al, 2014; Kong et al, 2016). …”

mentioning

confidence: 99%

Nitrifying and Denitrifying Bacterial Communities in Advanced Nitrogen‐Removal Onsite Wastewater Treatment Systems

Wigginton

Brannon²,

Kearns

et al. 2018

J of Env Quality

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Advanced N‐removal onsite wastewater treatment systems (OWTS) rely on nitrification and denitrification to remove N from wastewater. Despite their use to reduce N contamination, we know little about microbial communities controlling N removal in these systems. We used quantitative polymerase chain reaction and high‐throughput sequencing targeting nitrous oxide reductase (nosZ) and bacterial ammonia monooxygenase (amoA) to determine the size, structure, and composition of communities containing these genes. We analyzed water samples from three advanced N‐removal technologies in 38 systems in five towns in Rhode Island in August 2016, and in nine systems from one town in June, August, and October 2016. Abundance of nosZ ranged from 9.1 × 103 to 9 × 108 copies L−1 and differed among technologies and over time, whereas bacterial amoA abundance ranged from 0 to 1.9 × 107 copies L−1 and was not different among technologies or over time. Richness and diversity of nosZ—but not amoA—differed over time, with median Shannon diversity indices ranging from 2.61 in October to 4.53 in August. We observed weak community similarity patterns driven by geography and technology in nosZ. The most abundant nosZ‐ and amoA‐containing bacteria were associated with water distribution and municipal wastewater treatment plants, such as Nitrosomonas and Thauera species. Our results show that nosZ communities in N‐removal OWTS technologies differ slightly in terms of size and diversity as a function of time, but not geography, whereas amoA communities are similar across time, technology, and geography. Furthermore, community composition appears to be stable across technologies, geography, and time for amoA. Core Ideas We describe N‐cycling bacterial communities in advanced N‐removal septic systems. Geographic factors are weak drivers of community composition in nosZ. Technology design is a weak driver of community composition in nosZ. Community composition of amoA is similar across time, space, and among technologies Time drives differences in nosZ—but not amoA—diversity and abundance.

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Microbial structures and community functions of anaerobic sludge in six full-scale wastewater treatment plants as revealed by 454 high-throughput pyrosequencing

Cited by 278 publications

References 36 publications

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Bacterial community analysis of marine recirculating aquaculture system bioreactors for complete nitrogen removal established from a commercial inoculum

Nitrifying and Denitrifying Bacterial Communities in Advanced Nitrogen‐Removal Onsite Wastewater Treatment Systems

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