Bacterial community analysis of marine recirculating aquaculture system bioreactors for complete nitrogen removal established from a commercial inoculum

Abstract

An experimental recirculating aquaculture system was constructed under ambient seawater conditions to compare microbial community diversity of nitrifying and denitrifying biofilters that were derived from a commercial inoculum used for aquarium applications. Next generation sequencing revealed distinct and diverse microbial communities in samples analyzed from the commercial inoculant and the denitrification and nitrification biofilters. In all samples, communities were represented by a few dominant operational taxonomic units (OTUs). Bacteria having the capacity to carry out ammonia and nitrite oxidation were more abundant in the nitrification biofilter. Similarly, the proportion of the bacterial taxa known to carry out heterotrophic and autotrophic denitrification and participate in sulfur cycling were found in the denitrification bioreactor, and likely originated from the ambient environmental water source. Our results indicated that environmental seawater can be a favorable enhancement to the bacterial consortium of recirculating aquaculture systems biofilters.

Spatial and temporal axes impact ecology of the gut microbiome in juvenile European lobster (Homarus gammarus)

“…The unclassified ESVs limited to the LBC system and assigned to Sphingomonadaceae and Saprospiraceae could both represent biofouling species derived from the recirculating system. Sphingomonadaceae, a family of Alphaproteobacteria, have been identified in the guts of oriental river prawn (Macrobrachium nipponense) [75] but also isolated from fouled membranes of water filtration systems [76], and Saprospiraceae, a family of Bacteroidetes, have been isolated from shrimp rearing water [77,78] and recirculating systems [79,80] likely explaining their association with hatchery individuals. The Sphingomonadaceae ESV was also identical to a sequence derived from the guts of reared N. norvegicus (JN092211) [45].…”

Feed Supplementation with a Commercially Available Probiotic Solution Does Not Alter the Composition of the Microbiome in the Biofilters of Recirculating Aquaculture Systems

“…Likewise, Planctomycetes sp., Chloroflexi sp., Chitinophaga sp. and Rhizobiaceae were among the bacteria reported by Brailo et al following their screening of a pilot seawater biofilter unit [ 26 ]. In contrast, Bartelme et al have reported that Nitrosomonas sp., Nitrospira sp.…”

Macro and Micro Algae in Pollution Control and Biofuel Production – A Review

“…Therefore, they can play an important role during the final steps of wastewater treatment when phosphorous, nitrogen, and chemical oxygen demand (COD) have to be decreased . Bioremediation by microalgae for the disposal of industrial and urban waste is feasible for reducing the biological oxygen demand (BOD) and the amount of nutrients in wastewater .…”