Microbial sensors for determination of aromatics and their chloroderivatives. Part II: Determination of chlorinated phenols using a Rhodococcus-containing biosensor

Riedel, K.; Hensel, Jürgen; Rothe, Steffen; Neumann, Bettina; Scheller, Frieder W.

doi:10.1007/bf00242954

Cited by 39 publications

(9 citation statements)

References 15 publications

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“…In this context, bioassays based on enzymes [23,24,25,26,27,28], DNA [29,30], organelles [31], microorganisms [32,33,34,35] and antibodies [36,37,38,39,40,41,42] have proved to be selective, sensitive and fast options [43]. Tyrosinase [23,25,44], peroxidase [27,28,45], cytochrome P450 monooxygenase [46] and glucose oxidase [26] have been the most extensively researched enzymes for the development of biosensors selective for chlorophenolic compounds.…”

Section: Catalin Nistor · Jenny Emnéusmentioning

confidence: 99%

A capillary-based amperometric flow immunoassay for 2,4,6-trichlorophenol

Nistor

Emnéus

2003

Anal Bioanal Chem

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This paper describes the development of two different capillary-based heterogeneous competitive flow immunoassay formats (capillary flow injection immunoassay (CFIIA) and capillary sequential injection immunoassay (CSIIA)) for the determination of 2,4,6-trichlorophenol (2,4,6-TCP). The assays are based on the competition between the analyte and an analyte derivative labelled with the enzyme beta-galactosidase, for an anti-TCP antibody, followed by the injection of the mixture at equilibrium into a flow stream, where separation between the fractions bound and unbound to the antibody is performed in a glass capillary containing immobilised protein A. The antibody-tracer fraction retained inside the protein A capillary was measured by injection of 4-aminophenyl- beta- D-galactoside (4-APG), followed by amperometric detection of the enzymatically generated 4-aminophenol (4-AP), leading to a negative correlation between the signal and the analyte concentration. The two immunoassay formats were compared in terms of sensitivity and speed, giving IC(50) values of 1.41+/-0.03 and 1.64+/-0.07 micro g L(-1), detection limits of 0.2 and 0.4 micro g L(-1), and sample throughputs of 6 and 4 h(-1) for the CFIIA and CSIIA system, respectively. The influence of different interfering chlorophenolic compounds in the assay was minor, with only one exception (i.e. 2,4-dichlorophenol). In addition, different water matrices were tested (surface, tap, and rain water), showing that the matrix influence was negligible, except for rainwater, which resulted in a 30% increase in sensitivity. As a conclusion, the assay is suitable for the fast screening of TCP present at low concentration levels in water samples.

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Section: Catalin Nistor · Jenny Emnéusmentioning

confidence: 99%

A capillary-based amperometric flow immunoassay for 2,4,6-trichlorophenol

Nistor

Emnéus

2003

Anal Bioanal Chem

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“…On the other hand, polynuclear aromatic hydrocarbons (PAH) and, in particular, their chlorine derivatives belong to super toxicants and can exert toxic effect even in trace concentrations, so they can be detected only by highly sensitive analyzers (i.e., in this case, bioluminescent sensors or, as conventional techniques, chromatography, mass spectrometry and immunoassay). Electrochemical microbial biosensor systems for detection of a wide range of xenobiotics have been described (Riedel et al, 1993;Riedel et al, 1991). For example, an amperomteric biosensor for detection of monoaromatics was based on Rhodococcus cells and their extract (Riedel et al, 1991).…”

Section: Determination Of Hydrocarbons and Their Derivativesmentioning

confidence: 99%

The Microbial Cell Based Biosensors

Решетилов¹,

Iliasov²,

Reshetilova³

2010

Intelligent and Biosensors

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“…Consequently, quantitative determination of 4-CP is imperative for environmental control. Several methods for the determination of 4-CP, for example, HPLC [3,4], gas-chromatography [5] usually coupled with extraction, capillary electrophoresis [6], and electrochemical methods [7], including a modified bio-sensor [8,9] were reported. Although phenolic species can be readily oxidized at carbon electrodes, these electrodes are not suitable for electroanalysis due mainly to the electrode passivation by oxidation products.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Detection of 4‐Chlorophenol on Two Types of Expanded Graphite Based Composite Electrodes

et al. 2008

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The assessment of an expanded graphite-Ag-zeolite-epoxy composite (EG-Z-Ag-Epoxy) electrode for the determination of 4-chlorophenol (4-CP) is described and compared to the corresponding expanded graphite-epoxy composite (EG-Epoxy) electrode. Cyclic voltammetry was used to characterize the electrochemical behavior and determination of 4-CP at both electrodes in 0.1 M Na 2 SO 4 and 0.1 M NaOH supporting electrolytes. A substantial enhancement of sensitivity for the determination of 4-CP at the EG-Z-Ag-Epoxy electrode was reached by applying a chemical preconcentration step prior to voltammetric quantification. Also, under these last conditions the lowest limit of detection of 1 mM illustrates the analytical versatility of this electrode in a concentration range where aquatic 4-chlorophenol pollution is known to occur.

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Microbial sensors for determination of aromatics and their chloroderivatives. Part II: Determination of chlorinated phenols using a Rhodococcus-containing biosensor

Cited by 39 publications

References 15 publications

A capillary-based amperometric flow immunoassay for 2,4,6-trichlorophenol

A capillary-based amperometric flow immunoassay for 2,4,6-trichlorophenol

The Microbial Cell Based Biosensors

Amperometric Detection of 4‐Chlorophenol on Two Types of Expanded Graphite Based Composite Electrodes

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