Microbial Sensor for the Determination of Ascorbic Acid Based on the Iron-Oxidizing Activity of Thiobacillus Ferrooxidans.

Kurosawa, Hiroshi; Katô, Masahiko; Nakamura, Kazuo; Amano, Yoshifumi

doi:10.3136/fsti9596t9798.1.9

Cited by 2 publications

(4 citation statements)

References 17 publications

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“…Immobilization of microorganisms is carried out by adsorption on different supports. Thus, polypropylene membrane, paper, membrane filter, carbon paste electrode, or alginate gel , are used. For example, cultured cells of Proteus vulgaris were mixed with sodium alginate and spread on a glass plate to make a membrane, which then was immersed in a CaCl 2 solution for immobilization.…”

Section: 2 Immobilization Of Enzymes or Whole Cellsmentioning

confidence: 99%

“…However, the microbial sensor responded to ferrous sulfate and sodium sulfite, as well as to ascorbic acid. Thus, the sensor would be applicable to the measurements of samples containing various organic compounds but not ferrous iron or reducing agents other than ascorbic acid …”

Section: Analysis Of Vitaminsmentioning

confidence: 99%

“…A microbial sensor takes advantage of the ironoxidizing activity of Thiobacillus ferrooxidans. 137 By adding ascorbic acid to the solution of ferric iron, Fe 3+ reduces to Fe 2+ as ascorbic acid oxidizes to dehydroascorbic acid. The resulting Fe 2+ is oxidized by T. ferrooxidans but the ascorbic or dehydroascorbic acids are not.…”

Section: Analysis Of Vitaminsmentioning

confidence: 99%

“…Thus, the sensor would be applicable to the measurements of samples containing various organic compounds but not ferrous iron or reducing agents other than ascorbic acid. 137 A comparative study was carried out 29 on the advantages of four types of biosensors for ascorbic acid: (a) soluble enzyme (fixed directly on the electrode surface using a dyalisis membrane); (b) enzyme cross-linked with glutaraldehyde with or without egg albumin; (c) tissular electrode (slices of Cucumis sativus); (d) bacterial electrode (Enterobacter agglomerans). The soluble enzyme electrode is not useful because of its low stability, long response time, and higher influence on the response due to the temperature than the other biosensors.…”

Section: Analysis Of Vitaminsmentioning

confidence: 99%

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Application of Amperometric Biosensors to the Determination of Vitamins and α-Amino Acids

Alaejos

Montelongo

2004

Chem. Rev.

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8. Simultaneous Analysis of D-and L-Amino Acids 3259 4. Microdialysis Methods for Glutamic and Ascorbic Acids 3261 5. Conclusions 3263 6. References 3263 Maite Sanz Alaejos was born in Madrid, Spain. She received a M.Sc. in Biological Sciences from the University of La Laguna in 1977 and a M.Sc. in Pharmacy from the same university in 1981. She received her Ph.D. in Pharmacy under the supervision of Prof. Dr. F. J. Garcı ´a Montelongo and of Prof. Dr. J. C. Rodrı ´guez Placeres from the University of La Laguna in 1987. Her thesis work was on the polarographic determination of the stability constants of the simple and mixed coordinated species of Cu(II) with amino acids. Since 1990, she has been Assistant Professor in the Department of Analytical Chemistry, Nutrition and Food Sciences in the University of La Laguna. Prof. Francisco J. Garcı ´a Montelongo was born in Santa Cruz de Tenerife (Canary Islands, Spain). He received his M.Sc. (1961) and Ph.D. (1969) in Chemistry from the University of La Laguna. He was professor of Analytical Chemistry at the University of Bilbao (Spain) and since 1976 in the University of La Laguna. His main research interest is in the fields of biosensors, environmental pollution, and development of new analytical methodologies.

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