Microbial Ribosomal Vaccines

Gregory, Richard L.

doi:10.1093/clinids/8.2.208

Cited by 49 publications

(28 citation statements)

References 59 publications

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“…Ribosome vaccines are intriguing possibilities for immunization, as they are thought to be more immunogenic, less toxic, and more defined than whole-cell vaccines (51,52). Immunization with ribosomes could confer some cross-serotype protection, reducing the number of vaccines to be developed and delivered to patients (51,52).…”

Section: Discussionmentioning

confidence: 99%

“…Immunization with ribosomes could confer some cross-serotype protection, reducing the number of vaccines to be developed and delivered to patients (51,52). Early studies, mainly from the 1960s and 1970s, demonstrated the potential of ribosomal components as protective vaccines.…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that components of the ribosome itself (rRNA) or closely associated with the ribosome (recently translated protein and cell surface proteins) may act as adjuvants (51,52). A complex particle containing rRNA, proteins, and other closely associated molecules could be highly immunogenic and thus stimulate cellmediated as well as humoral immune responses (52).…”

Section: Discussionmentioning

confidence: 99%

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Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4⁺T Cells

et al. 2017

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Tuberculosis (TB) due to Mycobacterium tuberculosis remains a major global infectious disease problem, and a more efficacious vaccine is urgently needed for the control and prevention of disease caused by this organism. We previously reported that a genetically modified strain of Mycobacterium smegmatis called IKEPLUS is a promising TB vaccine candidate. Since protective immunity induced by IKEPLUS is dependent on antigen-specific CD4 ϩ T cell memory, we hypothesized that the specificity of the CD4 ϩ T cell response was a critical feature of this protection. Using in vitro assays of interferon gamma production (enzyme-linked immunosorbent spot [ELISPOT] assays) by splenocytes from IKEPLUS-immunized C57BL/6J mice, we identified an immunogenic peptide within the mycobacterial ribosomal large subunit protein RplJ, encoded by the Rv0651 gene. In a complementary approach, we generated major histocompatibility complex (MHC) class II-restricted T cell hybridomas from IKEPLUS-immunized mice. Screening of these T cell hybridomas against IKE-PLUS and ribosomes enriched from IKEPLUS suggested that the CD4 ϩ T cell response in IKEPLUS-immunized mice was dominated by the recognition of multiple components of the mycobacterial ribosome. Importantly, CD4 ϩ T cells specific for mycobacterial ribosomes accumulate to significant levels in the lungs of IKEPLUSimmunized mice following aerosol challenge with virulent M. tuberculosis, consistent with a role for these T cells in protective host immunity in TB. The identification of CD4 ϩ T cell responses to defined ribosomal protein epitopes expands the range of antigenic targets for adaptive immune responses to M. tuberculosis and may help to inform the design of more effective vaccines against tuberculosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4⁺T Cells

et al. 2017

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“…With the exceptions of lipopolysaccharide (LPS) (On0 et al, 1982(On0 et al, ,1984Jost et al, 1986 ;Farrelly et al, 1987), the individual antigens have not been identified or characterised. As with many other gram-negative bacteria (Gregory, 1986), it has proved extremely difficult to obtain preparations of individual antigens which are not contaminated with LPS (Kelson et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Expression of two conserved leptospiral antigens in Escherichia coli

Doherty

Adler²,

Rood³

et al. 1989

Journal of Medical Microbiology

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“…The DNA sequence in genes is copied into a messenger RNA and the ribosomes then read the information in this RNA and use it to create proteins 3 . Ribosomal vaccines have been assimilated from several different types of organisms and many of these preparations offer a higher degree of protection than do vaccines made from the homologous whole cells 4 . Bacterial lysates are mixtures of bacterial antigens derived from different inactivated pathogenic bacteria.…”

mentioning

confidence: 99%

Bacterial immunostimulants in recurrent paediatric respiratory infections

Perera¹

2011

Sri Lanka J Child Health

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Microbial Ribosomal Vaccines

Cited by 49 publications

References 59 publications

Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4⁺T Cells

Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4⁺T Cells

Expression of two conserved leptospiral antigens in Escherichia coli

Bacterial immunostimulants in recurrent paediatric respiratory infections

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Microbial Ribosomal Vaccines

Cited by 49 publications

References 59 publications

Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4+T Cells

Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4+T Cells

Expression of two conserved leptospiral antigens in Escherichia coli

Bacterial immunostimulants in recurrent paediatric respiratory infections

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Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4⁺T Cells

Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4⁺T Cells