Expression of two conserved leptospiral antigens in Escherichia coli

Doherty, Judy P.; Adler, Ben; Rood, Julian I.; Billington, Stephen J.; Faine, S.

doi:10.1099/00222615-28-2-143

Cited by 13 publications

(8 citation statements)

References 34 publications

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“…Whole-cell leptospiral DNA was prepared from serovar hardjo strain L171 as described previously (Marshall et al, 1984;Doherty et al, 1989). E. coli plasmid DNA was isolated by the alkaline lysis method (Birnboim & Doly, 1979).…”

Section: Methodsmentioning

confidence: 99%

Molecular analysis of a Leptospira borgpetersenii gene encoding an endoflagellar subunit protein

Mitchison

Rood

Faine

et al. 1991

Journal of General Microbiology

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Section: Methodsmentioning

confidence: 99%

Molecular analysis of a Leptospira borgpetersenii gene encoding an endoflagellar subunit protein

Mitchison

Rood

Faine

et al. 1991

Journal of General Microbiology

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“…A number of leptospiral genes have been cloned and analyzed, including several for amino acid synthesis (163,486,674), rRNA (228,229), ribosomal proteins (676), RNA polymerase (483), DNA repair (540), heat shock proteins (47, 441), sphingomyelinase (508,509), hemolysins (154,343), outer membrane proteins (168,255,256,515), flagellar proteins (354,355,398,584,640), and lipopolysaccharide (LPS) synthesis (88,152,317,397).…”

Section: Molecular Biologymentioning

confidence: 99%

Leptospirosis

2001

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Leptospirosis is a worldwide zoonotic infection with a much greater incidence in tropical regions and has now been identified as one of the emerging infectious diseases. The epidemiology of leptospirosis has been modified by changes in animal husbandry, climate, and human behavior. Resurgent interest in leptospirosis has resulted from large outbreaks that have received significant publicity. The development of simpler, rapid assays for diagnosis has been based largely on the recognition that early initiation of antibiotic therapy is important in acute disease but also on the need for assays which can be used more widely. In this review, the complex taxonomy of leptospires, previously based on serology and recently modified by a genotypic classification, is discussed, and the clinical and epidemiological value of molecular diagnosis and typing is also evaluated

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“…Few DNA sequences from the pathogenic species L. interrogans have been cloned and maintained in E. coli : DNA probes usable in taxonomic identification (LeFebvre, 1987;Van Eys et al, 1988;Zuerner & Bolin, 1988) isolated from Serovar hardjo colony libraries ; antigen genes from a serovar canicola colony library (Yamaguchi et al, 1988); two antigen genes from serovar pornonu (Doherty et al, 1989); and a 23s ribosomal RNA gene from serovar canicda (Fukunaga et al, 1989). This paper reports the first cloning of genes involved in amino acid biosynthetic pathways from a pathogenic Leptospira strain.…”

Section: Discussionmentioning

confidence: 99%

Cloning of genes required for amino acid biosynthesis from Leptospira interrogans serovar icterohaemorrhagiae

Richaud

Margarita²,

Baranton³

et al. 1990

Journal of General Microbiology

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Leptospiru interrogans belongs to a large family of important pathogens, which is part of the order Spirochaetales, a distinct group of eubacteria. In order to obtain a better understanding of the genetic organization of this species, we have constructed a DNA library of the Serovar kterohaemorrhaghze, using the Escherichia coli vector pUC13. We have isolated Leptospira DNA fragments containing the genetic information required to complement strains of E. coli with defects in proline and leucine biosynthesis. While a 3.9 kb fragment which complemented proA also complemented pruB, a 15 kb fragment complementing kuB could not complement other leu mutations. The L.interroguns origin of the cloned DNA fragments was confirmed by DNA-DNA hybridization. The hybridization was specific to the pathogenic species and was not seen with the saprophytic species L. bijkxu.

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Expression of two conserved leptospiral antigens in Escherichia coli

Cited by 13 publications

References 34 publications

Molecular analysis of a Leptospira borgpetersenii gene encoding an endoflagellar subunit protein

Molecular analysis of a Leptospira borgpetersenii gene encoding an endoflagellar subunit protein

Leptospirosis

Cloning of genes required for amino acid biosynthesis from Leptospira interrogans serovar icterohaemorrhagiae

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