Microbial production and downstream processing of 2,3-butanediol

Afschar, A. S.; Rossell, Carlos Eduardo Vaz; Jonas, Rainer; Chanto, Adolfo Quesada; Schaller, K.

doi:10.1016/0168-1656(93)90094-4

Cited by 65 publications

(36 citation statements)

References 9 publications

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“…CCZU11-1. These diols were important platform chemicals for the production of chemical intermediates and polymers [1][2][3][4]. As compared to other reported biocatalysts (e.g., exposide hydrolase, esterase, reductase, and oxidase) [8][9][10][11], sulfatase has unique biocatalytic properties for catalyzing these sulfates (1a-4a) to diols.…”

Section: Biotransformation With Toluene-permeabilized Cellsmentioning

confidence: 99%

“…Short-chain diols (e.g., ethylene glycol (EG), 1,2-propanediol (1,2-PD), and 1,3-propanediol (1,3-PD)) are one kind of important platform chemicals that serve as the basic starting materials for producing chemical intermediates, building blocks, and polymers [1][2][3][4][5]. The chemical synthesis of short-chain diols from petroleum materials has been employed for decades [6].…”

Section: Introductionmentioning

confidence: 99%

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Biotransformation of 1,3-Propanediol Cyclic Sulfate and Its Derivatives to Diols by Toluene-Permeabilized Cells of Bacillus sp. CCZU11-1

Liu

Zhang

et al. 2014

Appl Biochem Biotechnol

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In this study, it was the first report that Bacillus sp. CCZU11-1 was used for the biotransformation of 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives. The catalytic performance of Bacillus sp. sulfatase in the biotransformation of 1,3-PDS was significantly improved by biocatalyst permeabilization and immobilization. Using cell permeabilization, the hydrolytic activity of the whole-cell biocatalyst was increased by 3.5-fold after 1.5 h of pretreatment with 10 % (v/v) toluene at 30 °C and pH 7.0. Biotransformation of 20 mM 1,3-PDS for 24 h, 1,3-propanediol (1,3-PD) could be obtained in the yield of 97.4 % under the optimized reaction condition. Additionally, the immobilized biocatalysts, permeabilized cells entrapped in calcium alginate, and cross-linked enzyme aggregates were further employed to biotansform 1,3-PDS. Moreover, the total operational time of the immobilized biocatalysts could reach above 240 h with high conversion rate (>90 %).

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Section: Biotransformation With Toluene-permeabilized Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biotransformation of 1,3-Propanediol Cyclic Sulfate and Its Derivatives to Diols by Toluene-Permeabilized Cells of Bacillus sp. CCZU11-1

Liu

Zhang

et al. 2014

Appl Biochem Biotechnol

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“…Solvent extraction using ethyl acetate, diethyl ether and n-butanol has been investigated (Garg and Jain 1995) but the yield of 2,3-BD was relatively low. Besides the above mentioned methods, salting-out using K 2 CO 3 was also reported, but large amounts of salt were needed because the concentration of 2,3-BD in the fermentation broth was rather low (Afschar et al 1993;Xiu and Zeng 2008).…”

Section: Introductionmentioning

confidence: 98%

Aqueous two-phase extraction of 2,3-butanediol from fermentation broths by isopropanol/ammonium sulfate system

2008

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A novel aqueous two-phase system consisted of 2-propanol/ammonium sulfate was used for the extraction of 2,3-butanediol from fermentation broths. The maximum partition coefficient and recovery of 2,3-butanediol reached 9.9 and 93.7%, respectively, and more than 99% of the cells and about 85% of the soluble proteins were removed when 34% (w/w) 2-propanol and 20% (w/w) ammonium sulfate were used. The separated cells could be re-used as inocula for subsequent fermentations. The aqueous two-phase system described in this study may have potential application in the extraction of 2,3-butanediol produced by industrial fermentation processes.

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“…Native producers, such as Klebsiella pneumoniae [46], Klebsiella oxytoca [47] and Bacillus polymyxa [48], have been used for BDO production. However, the pathogenicity of opportunistic infection caused by the Klebsiella species is generally thought to be an obstacle hindering the large-scale 2,3-BDO production.…”

Section: 3-bdomentioning

confidence: 99%

Microbial production of short chain diols

et al. 2014

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Short chain diols (propanediols, butanediols, pentanediols) have been widely used in bulk and fine chemical industries as fuels, solvents, polymer monomers and pharmaceutical precursors. The chemical production of short chain diols from fossil resources has been developed and optimized for decades. Consideration of the exhausting fossil resources and the increasing environment issues, the bio-based process to produce short chain diols is attracting interests. Currently, a variety of biotechnologies have been developed for the microbial production of the short chain diols from renewable feed-stocks. In order to efficiently produce bio-diols, the techniques like metabolically engineering the production strains, optimization of the fermentation processes, and integration of a reasonable downstream recovery processes have been thoroughly investigated. In this review, we summarized the recent development in the whole process of bio-diols production including substrate, microorganism, metabolic pathway, fermentation process and downstream process.

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Microbial production and downstream processing of 2,3-butanediol

Cited by 65 publications

References 9 publications

Biotransformation of 1,3-Propanediol Cyclic Sulfate and Its Derivatives to Diols by Toluene-Permeabilized Cells of Bacillus sp. CCZU11-1

Biotransformation of 1,3-Propanediol Cyclic Sulfate and Its Derivatives to Diols by Toluene-Permeabilized Cells of Bacillus sp. CCZU11-1

Aqueous two-phase extraction of 2,3-butanediol from fermentation broths by isopropanol/ammonium sulfate system

Microbial production of short chain diols

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