Microbial Interactions Drive Distinct Taxonomic and Potential Metabolic Responses to Habitats in Karst Cave Ecosystem

Ma, Liyuan; Huang, Xinping; Wang, Hongmei; Yun, Yuan; Cheng, Xiaoyu; Liu, Deng; Lu, Xiaolu; Qiu, Xuan

doi:10.1128/spectrum.01152-21

Cited by 27 publications

(32 citation statements)

References 64 publications

(87 reference statements)

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“…Highly diverse bacterial communities were detected from the weathered rocks in caves with the dominance of Proteobacteria , Actinomycetes , and Firmicutes ( Yun et al, 2016 ; Zhu et al, 2019 , 2021 ; Ma et al, 2021 ). In contrast, the diversity of fungi is relatively low due to the limited input of organic matter by photosynthesis in caves ( Lee et al, 2012 ; Man et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

The Characterization of Microbiome and Interactions on Weathered Rocks in a Subsurface Karst Cave, Central China

et al. 2022

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Karst caves are a natural oligotrophic subsurface biosphere widely distributed in southern China. Despite the progress in bacterial and fungal diversity, the knowledge about interactions between bacteria, fungi, and minerals is still limited in caves. Hence, for the first time, we investigated the interaction between bacteria and fungi living on weathered rocks in the Heshang Cave via high-throughput sequencing of 16S rRNA and ITS1 genes, and co-occurrence analysis. The mineral compositions of weathered rocks were analyzed by X-ray diffraction. Bacterial communities were dominated by Actinobacteria (33.68%), followed by Alphaproteobacteria (8.78%), and Planctomycetia (8.73%). In contrast, fungal communities were dominated by Sordariomycetes (21.08%) and Dothideomycetes (14.06%). Mineral substrata, particularly phosphorus-bearing minerals, significantly impacted bacterial (hydroxyapatite) and fungal (fluorapatite) communities as indicated by the redundancy analysis. In comparison with fungi, the development of bacterial communities was more controlled by the environmental selection indicated by the overwhelming contribution of deterministic processes. Co-occurrence network analysis showed that all nodes were positively linked, indicating ubiquitous cooperation within bacterial groups and fungal groups, as well as between bacteria and fungi under oligotrophic conditions in the subsurface biosphere. In total, 19 bacterial ASVs and 34 fungal OTUs were identified as keystone taxa, suggesting the fundamental role of fungi in maintaining the microbial ecosystem on weathered rocks. Ascomycota was most dominant in keystone taxa, accounting for 26.42%, followed by Actinobacteria in bacteria (24.53%). Collectively, our results confirmed the highly diverse bacterial and fungal communities on weathered rocks, and their close cooperation to sustain the subsurface ecosystem. Phosphorus-bearing minerals were of significance in shaping epipetreous bacterial and fungal communities. These observations provide new knowledge about microbial interactions between bacteria, fungi, and minerals in the subterranean biosphere.

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Section: Introductionmentioning

confidence: 99%

The Characterization of Microbiome and Interactions on Weathered Rocks in a Subsurface Karst Cave, Central China

et al. 2022

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“…3 and Table A4, Appendix A -Supplementary data). Both phyla are cosmopolitan and appeared frequently in oligotrophic sediments, but there exists relatively little information about their ecological role (Eichorst et al, 2018;Ma et al, 2021). Collectively, our findings suggest that moonmilk deposit formation in Pindal Cave is closely related to Crossiella and wb1-P19.…”

Section: Bacterial Communities On Cave Formations: From Sediments To ...mentioning

confidence: 75%

“…To the best of our knowledge, there is no study associating Crossiella relative abundance with moonmilk formation, except in the study on a volcanic cave where Crossiella relative abundance was 2.5% (Gonzalez-Pimentel et al, 2021). However, a few authors reported the finding of Crossiella not only in carbonate environments such as weathered rock caves (Ma et al, 2021), but also in deteriorated stone monuments (Li et al, 2018).…”

Section: Bacterial Communities On Cave Formations: From Sediments To ...mentioning

confidence: 99%

Role of Subterranean Microbiota in the Carbon Cycle and Greenhouse Gas Dynamics

Martin-Pozas¹,

Cuezva²,

Fernández-Cortés³

et al. 2022

SSRN Journal

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“…In an acidic cave system, dominant species of bacteria displayed high variability in different microhabitats as a function of pH (Yun et al., 2016). However, how microorganisms inhabit a hyperalkaline environment remains underexplored, especially in a dark and oligotrophic cave setting (Dong et al., 2020; Ma et al., 2021).…”

Section: Introductionmentioning

confidence: 99%

Distinct Microbial Communities in the Soils, Waters, and Speleothems of a Hyperalkaline Cave System

et al. 2022

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Cave and karst systems represent some of the least studied extreme environments on the planet. Caves are in most cases characterized by extreme nutrient deficiency and utter darkness (Dong et al., 2020), characteristics quite inhospitable to life. However, understanding the karst critical zone (Chen et al., 2018) is of fundamental importance to vast numbers of the global population who rely on karst aquifers for fresh drinking water supplies (Stevanovic, 2019). Possibly the least well-established connections within karst and cave science revolve around the role of microbial communities within karst, their sources, abundance, community structure and their ability to adapt and even thrive in cave environments (Engel, 2010). It is possible that microbial communities play a central role in the karst aquifer system; their utilisation and consumption of key nutrients (C, N, S, and P) may help to balance the anthropogenic increase in nutrient loads to many karst environments. With microbes holding such a

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Microbial Interactions Drive Distinct Taxonomic and Potential Metabolic Responses to Habitats in Karst Cave Ecosystem

Cited by 27 publications

References 64 publications

The Characterization of Microbiome and Interactions on Weathered Rocks in a Subsurface Karst Cave, Central China

The Characterization of Microbiome and Interactions on Weathered Rocks in a Subsurface Karst Cave, Central China

Role of Subterranean Microbiota in the Carbon Cycle and Greenhouse Gas Dynamics

Distinct Microbial Communities in the Soils, Waters, and Speleothems of a Hyperalkaline Cave System

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