Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier

Ge, Li; Qi, Jie; Shao, Bo; Ruan, Zhenzhen; Ren, Yueran; Sui, Shujing; Wu, Xinpei; Sun, Xueqiang; Liu, Shuman; Li, Sha; Xu, Chi; Song, Wengang

doi:10.1080/19490976.2021.2013764

Cited by 37 publications

(40 citation statements)

References 58 publications

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“…Both, our in silico and in vitro results showed that C. jejuni M1cam has the ability to utilise H

as an energy source. The genome encodes several hydrogenase enzymes, both soluble and membrane bound, and the ability to utilise H

might bring a competitive advantage to its survival or niche adaptation in the gut environment which is known to contain H

-producing bacteria [ 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam

et al. 2022

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Campylobacter jejuni, the major cause of bacterial foodborne illness, is also a fastidious organism that requires strict growth requirements in the laboratory. Our aim was to study substrate utilisation and energy metabolism in non-growing C. jejuni to investigate the ability of these bacteria to survive so effectively in the food chain. We integrated phenotypic microarrays and genome-scale metabolic modelling (GSM) to investigate the survival of C. jejuni on 95 substrates. We further investigated the underlying metabolic re-adjustment associated with varying energy demands on each substrate. We identified amino acids, organic acids and H2, as single substrates supporting survival without growth. We identified several different mechanisms, which were used alone or in combination, for ATP production: substrate-level phosphorylation via acetate kinase, the TCA cycle, and oxidative phosphorylation via the electron transport chain that utilised alternative electron donors and acceptors. The benefit of ATP production through each of these mechanisms was associated with the cost of enzyme investment, nutrient availability and/or O2 utilisation. C. jejuni can utilise a wide range of substrates as energy sources, including organic acids commonly used for marination or preservation of ingredients, which might contribute to the success of their survival in changing environments.

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“…Both, our in silico and in vitro results showed that C. jejuni M1cam has the ability to utilise H

as an energy source. The genome encodes several hydrogenase enzymes, both soluble and membrane bound, and the ability to utilise H

might bring a competitive advantage to its survival or niche adaptation in the gut environment which is known to contain H

-producing bacteria [ 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam

et al. 2022

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“…16 It has been shown to instruct colonocytes to consume oxygen through the β-oxidation metabolic pathway and consequently protect the host against the expansion of potentially pathogenic bacteria that can lead to IBD. 17 Experimentally, butyrate induces the barrier function in T84 cells, but the influence is lost in cells lacking HIF, which demonstrates the importance of HIF in maintaining barrier integrity. A protective role for butyrate on HIF-regulated barrier function has also been proposed.…”

Section: Short-chain Fatty Acids Affect the Intestinal Barrier In Inf...mentioning

confidence: 95%

“…have found microbial‐derived butyrate could prompt oxygen consumption in intestinal epithelial cell lines and stabilize hypoxia‐inducible factor (HIF), a transcription factor that protects the intestinal barrier 16 . It has been shown to instruct colonocytes to consume oxygen through the β‐oxidation metabolic pathway and consequently protect the host against the expansion of potentially pathogenic bacteria that can lead to IBD 17 . Experimentally, butyrate induces the barrier function in T84 cells, but the influence is lost in cells lacking HIF, which demonstrates the importance of HIF in maintaining barrier integrity.…”

Section: Short‐chain Fatty Acids Affect the Intestinal Barrier In Inf...mentioning

confidence: 99%

Short‐chain fatty acids affect the development of inflammatory bowel disease through intestinal barrier, immunology, and microbiota: A promising therapy?

Peng

Xia

Xiao

et al. 2022

J of Gastro and Hepatol

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Intestinal metabolites are attracting increasing interest, especially more and more studies have found they are closely related to diseases. Microbial fermentation of indigestible dietary fibers in the gut produces short chain fatty acids (SCFAs) as the main product. SCFAs can exert influences on the integrity of the intestinal epithelial and mucosal barrier, immune reactions, and the diversity of microbiota in humans. Thus, alteration in SCFAs may affect inflammatory bowel disease (IBD). In IBD, SCFAs are involved in the main pathogenic process and play an important role in the development of intestinal inflammation. Although many studies have proved that pretreatment with SCFAs can effectively ameliorate inflammation in the gut, the mechanisms are not fully understood. In this review, we describe the relationship between SCFAs and IBD from the aspects of defense barrier, immune effects, and microbial alterations. We also summarize the effects of SCFAs on comorbidities in IBD via the gut-brain, gut-liver, and gut-lung axis, and we give an overview of the prospects of their clinical application. A better understanding of the relevance of SCFAs in IBD may reveal novel targets for future study

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“…Biyofilm yapısında en çok bulunan üçüncü mikroorganizma grubunu Lachnospiraceae oluşturmaktadır. Bu gruptaki bakteriler bütirat üreten anerobik bakteriler olarak bilinmektedir [22]. Clostridium türleri H2 üretimi ile ilgili çalışmalarda en sık rastlanan fermentative anaerobik bakteriler arasında yer almaktadır.…”

Section: H2 üRetimi Ve Mikrobiyal Dağılım Arasındaki Ilişkiunclassified

Elma posasından biyohidrojen üretimine farklı yükleme oranlarının etkisi

Muratçobanoğlu

Gökçek

Baş

et al. 2022

NÖHÜ Müh. Bilim. Derg.

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Küresel çevre problemleri ve modern dünyanın artan enerji talebi alternatif ve yenilenebilir enerji kaynakları arayışını ortaya çıkarmıştır. Alternatif enerji kaynaklarının başında ise yüksek enerji içeriğine sahip olması, enerji taşıyıcısı olması ve çevre dostu olması gibi avantajları nedeniyle hidrojen gelmektedir. Hidrojen üretim prosesleri; elektrokimyasal yöntemler, termal yöntemler ve biyolojik hidrojen üretimi olarak sıralanabilir. Bu yöntemler arasında daha ekonomik ve sürdürülebilir olması nedeniyle biyolojik yöntemler ön plana çıkmaktadır. Bu çalışmada, biyolojik bir proses olan karanlık fermantasyon yöntemi ile elma posasından biyohidrojen üretimi incelenmiştir. Elma posası kullanılarak, aşı çamuruna uygulanan fiziksel ön işlem (90°C- 30 dk) sonrası farklı substrat/aşı (S/I) yükleme oranlarında kesikli reaktörlerden elde edilen biyohidrojen verimleri karşılaştırılmıştır. En iyi performansı gösteren S/I 2 yükleme koşullarında 208 ml/gUK biyogaz ve 18 mlH2/gUK biyohidrojen üretimi gerçekleşmiştir. Mikrobiyal dağılım sonuçları incelendiğinde Lactobacillus, Clostridium ve Lachnospiraceae türlerinin en baskın türler olduğu görülmektedir.

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Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier

Cited by 37 publications

References 58 publications

Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam

Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam

Short‐chain fatty acids affect the development of inflammatory bowel disease through intestinal barrier, immunology, and microbiota: A promising therapy?

Elma posasından biyohidrojen üretimine farklı yükleme oranlarının etkisi

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