Microbial flora and antimicrobial susceptibility patterns from dogs with otitis media

Colombini, S.; Merchant, Sandra R.; Hosgood, Giselle

doi:10.1046/j.1365-3164.2000.00191.x

Cited by 44 publications

(36 citation statements)

References 12 publications

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“…The results of this study are similar to other described in the literature (2,3,4). Although some studies have considered cogulase-negative Staphylococci to be the main bacterial agent in canine otitis (6), in the present study CPS were the most common isolated agents.…”

supporting

confidence: 93%

Etiology of canine otitis media and antimicrobial susceptibility of coagulase-positive Staphylococci in Fortaleza city, Brazil

Oliveira¹,

Leite²,

Brilhante³

et al. 2006

Braz. J. Microbiol.

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This study evaluated the middle ear microbiota and antimicrobial susceptibility patterns from strains isolated from dogs with otitis media. A total of 62 dogs obtained from Zoonoses Control Center of Fortaleza CityCeará State / Brazil were studied over a 10-month period (August/2003 to June/2004. Of the total, 46.8% (n=30) of the animals were positive for otitis media and the infection was monomicrobial in 76.6% of them. The most frequent isolated agents were coagulase-positive Staphylococci (CPS-55%) and Pseudomonas sp (10%). For S. intermedius (n=13) and S. aureus subsp aureus (n=9), respectively, the greater resistance rates were observed using penicillin G (30.76% and 44.44%), ampicillin (7.69% and 44.44%), erythromycin (23.07% and 44.44%), clindamycin (23.07% and 44.44%) and thrimethropim/sulfamethoxazol (15.38% and 33.33%). Key words: otitis media, dogs, Staphylococci, antimicrobialsIn the animal practice, otitis media is a common disease and often a frustating problem. It is difficult to diagnose with the work-up being both costly and, at times invasive. In dogs the most common cause of otitis media is an extension from otitis externa and bacterial infection is more common than yeast infections (9). Cole et al.(2) compared the results of bacteriologic cultures from middle ear and the horizontal ear canal and identical isolates were found in only 10.5% of the ears. Sometimes empiric therapy is necessary, mainly in therapeutic-centers where microbiology laboratories are not available and when the invasive diagnostic is impossible to be done, as in the majority of cases of otitis media. Although predictable patterns exist, significant variation in resistance rates between medical institutions or geographical areas have been reported. Considering the great importance of the middle ear otitis in the veterinarian clinic and the few microbiological studies, the present study had as purposes to determine the etiology of canine otitis media and to evaluate the susceptibility profile of the prevalent bacteria. The criteria for the inclusion in the study were: otoscopic abnormal or ruptured tympanic membranes, otitis externa (with erythema, ulceration of the tegument, otorrhoeae and foreign bodies). The exudates of the middle ear were obtained after ear canal ablation and lateral bulla osteotomy. A 0.5 mL of BHI was injected with a syringe in the middle ear and the aspirated material was sent to the laboratory. Total transfer time to the laboratory was no longer than two hours. The specimens were inoculated onto blood agar (BAP), MacConkey agar and Brain Heart Infusion broth (BHI) and incubated at 37ºC in aerobiosis. The sample obtained for anaerobic culture was inoculated into supplemented Brain Heart Infusion agar (with 5% of sheep blood, hemine (5 μg/mL) and menadione (1 μg/mL) -and Bacteroides Bile Esculin agar and incubated at 35-37ºC in anaerobiosis. For fungal isolation, the samples were inoculated into Sabouraud glucose agar

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supporting

confidence: 93%

Etiology of canine otitis media and antimicrobial susceptibility of coagulase-positive Staphylococci in Fortaleza city, Brazil

Oliveira¹,

Leite²,

Brilhante³

et al. 2006

Braz. J. Microbiol.

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“…Isolates included in the study from 2011 were all collected from the ears of dogs with otitis externa, and it is well known that otic isolates tend to show higher resistance rates to several antimicrobials, including enrofloxacin and other fluoroquinolones (PETERSEN et al, 2002;WILDERMUTH et al, 2007;HARADA et al, 2012). Nevertheless, the sensitivity rate to enrofloxacin found in this study was still lower than sensitivity rates observed in other studies, where susceptibility ranged between 16% and 51% (COLOMBINI et al, 2000;MARTIN BARRASA et al, 2000;McKAY et al, 2007;WERCKENTHIN et al, 2007).…”

Section: Discussionmentioning

confidence: 49%

Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

2017

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________________________________________________________________________________________ PINTARIĆ, S., K. MATANOVIĆ, B. ŠEOL MARTINEC:Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs -comparing disk diffusion and microdilution methods. Vet. arhiv 87, 291-300, 2017. ABSTRACTPseudomonas aeruginosa has been determined to be the distinct cause of a number of different infections in both humans and animals. Apart from its high intrinsic resistance, which makes it difficult to treat, it also has a great capacity to acquire further resistance mechanisms. These mechanisms can be present simultaneously in one cell, conferring a multiresistant phenotype. Taking into account that the known risk factor for selection of resistant strains is excessive and inappropriate drug use, especially fluoroquinolones, the aim of this study was to determine the susceptibility of P. aeruginosa isolates to these antibacterial agents. The susceptibility of 90 canine isolates was determined by disk diffusion susceptibility test and the broth microdilution method. Both methods showed that isolates were significantly more sensitive to ciprofloxacin than to marbofloxacin or enrofloxacin, and more sensitive to marbofloxacin than to enrofloxacin. The results of microdilution and disk diffusion testing were in 98.9% agreement for ciprofloxacin and in 91.1% agreement for marbofloxacin, with no statistically significant difference between the two methods (P>0.05) for these two antibiotics. For enrofloxacin, the results of microdilution and disk diffusion testing were in 76.6% agreement, with a statistically significant disagreement between the two methods (P<0.05). In 16 cases, the disk diffusion test overestimated the number of strains susceptible to enrofloxacin compared with the microdilution test. These results suggest that for P. aeruginosa infections in dogs, therapy using enrofloxacin, based on the results of disk diffusion testing, may lead to ineffective treatment and could contribute to selection of resistant and multiresistant strains.

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“…The aminoglycosides, polymyxins, detergents, and most alcohols routinely used in the treatment of the external ear canal are known to be toxic to the nervous structures of the inner ear [11]. Potentially ototoxic antimicrobial pharmaceutics are present in most topical formulations for treatment of otitis externa.…”

Section: Ototoxicitymentioning

confidence: 99%

Diagnosis and treatment of otitis media in dogs and cats

Gotthelf¹

2004

Veterinary Clinics of North America: Small Animal Practice

111

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Microbial flora and antimicrobial susceptibility patterns from dogs with otitis media

Cited by 44 publications

References 12 publications

Etiology of canine otitis media and antimicrobial susceptibility of coagulase-positive Staphylococci in Fortaleza city, Brazil

Etiology of canine otitis media and antimicrobial susceptibility of coagulase-positive Staphylococci in Fortaleza city, Brazil

Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

Diagnosis and treatment of otitis media in dogs and cats

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