Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms

Zhao, Juntao; Li, Feng; Cao, Yingxiu; Zhang, Xinbo; Chen, Tao; Song, Hao; Wang, Zhiwen

doi:10.1016/j.biotechadv.2020.107682

Cited by 178 publications

(88 citation statements)

References 171 publications

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“…This creates a proton electrochemical gradient [19]. Keeping in mind that the electron transport chain is physically separated from the outside environment by cytoplasmic membranes with additional layers, such as cell walls, peptidoglycans, or outer membranes the physical transfer of the biologically liberated/created electrons to the surface of the electrode also plays an important critical role [20,21]. Mechanisms for transferring electrons from the microbial intracellular compartments to the surface of the electrode have been studied predominantly in bacterial systems; thus the use of bacteria is more common in the construction of mi-crobial fuel cell technology.…”

Section: Extracellular Electron Transfermentioning

confidence: 99%

Microbial Electrochemical Systems: Principles, Construction and Biosensing Applications

Hassan

Febbraio

Andreescu

2021

Sensors

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Microbial electrochemical systems are a fast emerging technology that use microorganisms to harvest the chemical energy from bioorganic materials to produce electrical power. Due to their flexibility and the wide variety of materials that can be used as a source, these devices show promise for applications in many fields including energy, environment and sensing. Microbial electrochemical systems rely on the integration of microbial cells, bioelectrochemistry, material science and electrochemical technologies to achieve effective conversion of the chemical energy stored in organic materials into electrical power. Therefore, the interaction between microorganisms and electrodes and their operation at physiological important potentials are critical for their development. This article provides an overview of the principles and applications of microbial electrochemical systems, their development status and potential for implementation in the biosensing field. It also provides a discussion of the recent developments in the selection of electrode materials to improve electron transfer using nanomaterials along with challenges for achieving practical implementation, and examples of applications in the biosensing field.

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Section: Extracellular Electron Transfermentioning

confidence: 99%

Microbial Electrochemical Systems: Principles, Construction and Biosensing Applications

Hassan

Febbraio

Andreescu

2021

Sensors

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“…Molecular biology techniques helped to clarify the pathways for electron transfer steps and also provide the possiblity to engineer microorganisms to use biomass as fuels for electricity generation. So far, various technical approaches including random approaches (i.e., directed evolution of redox enzymes, and silver/gold coating of cells), rational design (i.e., heterologous gene expression, engineering of metabolic processes, and engineering of bacterial pili), and de-novo design (i.e., bacterial surface display of redox proteins, yeast surface display of redox proteins, and hybrid MFC-enzyme based fuel cells) have been investigated for the genomic engineering of a novel or optimized biocatalysis in MFCs (Alfonta 2010;Zhao et al 2020). Recently, Li et al (2019) designed a bioengineered microbial consortium of Klebsiella pneumonia-S. oneidensis for efficiently harvesting of the electricity from corn stalk hydrolysate.…”

Section: Genetically Engineered Microorganismsmentioning

confidence: 99%

Recent advances on biomass-fueled microbial fuel cell

Moradian

Fang

Yong

2021

Bioresour. Bioprocess.

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Biomass is one of the most abundant renewable energy resources on the earth, which is also considered as one of the most promising alternatives to traditional fuel energy. In recent years, microbial fuel cell (MFC) which can directly convert the chemical energy from organic compounds into electric energy has been developed. By using MFC, biomass energy could be directly harvested with the form of electricity, the most convenient, wide-spread, and clean energy. Therefore, MFC was considered as another promising way to harness the sustainable energies in biomass and added new dimension to the biomass energy industry. In this review, the pretreatment methods for biomass towards electricity harvesting with MFC, and the microorganisms utilized in biomass-fueled MFC were summarized. Further, strategies for improving the performance of biomass-fueled MFC as well as future perspectives were highlighted.

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“…Although MES represents a promising platform for the renewable energy storage and value-added chemical production, it is currently limited by low electron transfer rates from a cathode to microbes ( Kracke et al, 2015 ; Chen et al, 2020b ; Zhao et al, 2020 ). Electron shuttles (ESs) provide an effective conduit for the extracellular electron transfer (EET) process from the electrode to microbial cells.…”

Section: Introductionmentioning

confidence: 99%

“…The changes in molecular structure of ESs by the substituent group typically affect their redox potentials, thereby influencing the electron transfer ( Rauschnot et al, 2009 ; Chen et al, 2013 ; Er et al, 2015 ). In addition, although several efforts have focused on the direct electron uptake mechanisms from cathodes to microbes in recent years ( Kracke et al, 2015 ; Rowe et al, 2018 ; Zhao et al, 2020 ), little is known about the underlying molecular mechanisms of ESs-mediated EET process in MES.…”

Section: Introductionmentioning

confidence: 99%

Tuning Redox Potential of Anthraquinone-2-Sulfonate (AQS) by Chemical Modification to Facilitate Electron Transfer From Electrodes in Shewanella oneidensis

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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Bioelectrochemical systems (BESs) are emerging as attractive routes for sustainable energy generation, environmental remediation, bio-based chemical production and beyond. Electron shuttles (ESs) can be reversibly oxidized and reduced among multiple redox reactions, thereby assisting extracellular electron transfer (EET) process in BESs. Here, we explored the effects of 14 ESs on EET in Shewanella oneidensis MR-1, and found that anthraquinone-2-sulfonate (AQS) led to the highest cathodic current density, total charge production and reduction product formation. Subsequently, we showed that the introduction of -OH or -NH2 group into AQS at position one obviously affected redox potentials. The AQS-1-NH2 exhibited a lower redox potential and a higher Coulombic efficiency compared to AQS, revealing that the ESs with a more negative potential are conducive to minimize energy losses and improve the reduction of electron acceptor. Additionally, the cytochromes MtrA and MtrB were required for optimal AQS-mediated EET of S. oneidensis MR-1. This study will provide new clues for rational design of efficient ESs in microbial electrosynthesis.

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Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms

Cited by 178 publications

References 171 publications

Microbial Electrochemical Systems: Principles, Construction and Biosensing Applications

Microbial Electrochemical Systems: Principles, Construction and Biosensing Applications

Recent advances on biomass-fueled microbial fuel cell

Tuning Redox Potential of Anthraquinone-2-Sulfonate (AQS) by Chemical Modification to Facilitate Electron Transfer From Electrodes in Shewanella oneidensis

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