Microbial Enzyme Engineering: Applications and Perspectives

Kumar, Vishal; Líu, Hao; Shukla, Pratyoosh

doi:10.1007/978-981-10-5275-0_13

Cited by 16 publications

(7 citation statements)

References 49 publications

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“…pH 5, 30 °C, 20 mg L − 1 BPA and 1500 U L − 1 laccase). Researchers have exploited numerous classes of enzymes and found that oxidoreductases including laccases (EC 1.10.3.2), manganese peroxidase (EC 1.11.1.13) and lignin peroxidase (EC 1.11.1.14), horseradish peroxidase (EC 1.11.1.7) and polyphenol oxidases are capable of degradation of EDCs (Becker et al 2017;Llorca et al 2017;Kumar et al 2017). The rate of degradation of EDCs can be increased by various redox mediators, inducers, surfactants and other additives.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous laccase production and transformation of bisphenol-A and triclosan using Trametes versicolor

et al. 2019

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New age micro-pollutants, bisphenol-A (BPA) and triclosan (TCA), known for their carcinogenic effects in living organisms can effectively be removed from water using laccase from Trametes versicolor. Laccase was produced from T. versicolor JSRK13 in both submerged and solid-state fermentation (SmF and SSF) conditions. In SmF, T. versicolor JSRK13 gave the maximum production of laccase on the 10th day with an activity of 22 U mL − 1 , whereas, in SSF 185 U g − 1 of the enzyme was produced on the 17th day. Maximum production of laccase was observed with Parthenium as substrate. Parthenium, with a particle size of 3-5 mm having 60% moisture was found to be a suitable substrate for laccase production and simultaneous transformation (LPST) of BPA in a synergistic manner. A one-step concentration using 85% ammonium sulphate followed by dialysis was sufficient to give 6.7-fold purification of laccase from the crude culture filtrate. Transformation of BPA was achieved in both SmF and SSF conditions along with the production of laccase, whereas TCA was degraded with free enzyme only. Above 90% of BPA (55-5 mg L − 1) was degraded using the LPST strategy with HBT acting as a mediator in the reaction. LPST strategy did not work for TCA as it completely inhibits the growth of T. versicolor JSRK13. TCA was degraded up to 75% (1.5-0.375 mg L − 1) by the free enzyme. Our study of simultaneous laccase production and transformation proved to be efficacious in case of BPA. The results indicate that industrial and sewage wastewater containing BPA can potentially be treated with T. versicolor JSRK13 laccase. The described strategy can further be used to develop a bioprocess which can work both on solid and liquid wastes containing BPA.

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Section: Introductionmentioning

confidence: 99%

Simultaneous laccase production and transformation of bisphenol-A and triclosan using Trametes versicolor

et al. 2019

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“…Though various types of bacterial EPSs has been isolated from different sources and are characterized over the last two decades, but only a few have practical applications, the reason being most of the newly characterized bacterial EPSs have no novel properties. Recent trends of microbial secondary metabolites have taken a turn where nanotechnology is playing a dominant role whether it is enzyme engineering [15] or bacteria derived biopolymers [16]. Characterization of EPS synthesized by extremophiles may open opportunities for unique applications.…”

Section: Introductionmentioning

confidence: 99%

Structural and Functional Properties of Exopolysaccharide Excreted by a Novel Bacillus anthracis (Strain PFAB2) of Hot Spring Origin

et al. 2017

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Exopolysaccharide produced by a unique avirulent strain PFAB2 of hot spring origin has been characterized and its functional properties are investigated which is a first report. Maximum yield of EPS is 7.66 g/l with 2% glucose and 1% peptone as optimum carbon and nitrogen source respectively. The EPS is found to be a homopolymer consisting of only glucose as principle monosaccharide component. ThroughH NMR study, different dextran-like proton peaks are observed. Molecular weight of the EPS resembles low molecular weight bacterial origin polysaccharides. Melting transition of the EPS has started after 276 °C which indicates good thermal stability. The EPS also shows potent antioxidant activity in terms of DPPH and ABTS mediated free radical scavenging property compared to standard ascorbic acid. Emulsifying property of the EPS is also observed and has shown good emulsification of vegetable oils. The polysaccharide forms a thermo resistant gel during the heating phase, with G' higher than G″ indicating excellent shear-thinning behaviour and viscoelastic nature of the EPS.

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“…Apart from gene editing, there has also been simultaneous progress in the development of protein engineering techniques or computational tools to tweak proteins or peptides, to be able to meet the growing industrial demands [ 71 , 72 ]. Such approaches are being recently exploited for the screening of new peptide sequences with antimicrobial properties, or for the computer-aided discovery of AMPs [ 73 - 75 ].…”

Section: Future Perspectivesmentioning

confidence: 99%

Antimicrobial Peptides: Recent Insights on Biotechnological Interventions and Future Perspectives

Sinha¹,

Shukla

2019

PPL

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With the unprecedented rise of drug-resistant pathogens, particularly antibiotic-resistant bacteria, and no new antibiotics in the pipeline over the last three decades, the issue of antimicrobial resistance has emerged as a critical public health threat. Antimicrobial Peptides (AMP) have garnered interest as a viable solution to this grave issue and are being explored for their potential antimicrobial applications. Given their low bioavailability in nature, tailoring new AMPs or strategizing approaches for increasing the yield of AMPs, therefore, becomes pertinent. </P><P> The present review focuses on biotechnological interventions directed towards enhanced AMP synthesis and revisits existing genetic engineering and synthetic biology strategies for production of AMPs. This review further underscores the importance and potential applications of advanced gene editing technologies for the synthesis of novel AMPs in future.

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Microbial Enzyme Engineering: Applications and Perspectives

Cited by 16 publications

References 49 publications

Simultaneous laccase production and transformation of bisphenol-A and triclosan using Trametes versicolor

Simultaneous laccase production and transformation of bisphenol-A and triclosan using Trametes versicolor

Structural and Functional Properties of Exopolysaccharide Excreted by a Novel Bacillus anthracis (Strain PFAB2) of Hot Spring Origin

Antimicrobial Peptides: Recent Insights on Biotechnological Interventions and Future Perspectives

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