Microbial enhanced oil recovery potential of surfactin-producing Bacillus subtilis AB2.0

Alvarez, Vanessa Marques; Guimarães, Carolina Reis; Jurelevicius, Diogo; Castilho, Livia Vieira Araújo de; Sousa, Joab Sampaio de; Mota, Fábio Faria da; Freire, Denise Maria Guimarães; Seldin, Lucy

doi:10.1016/j.fuel.2020.117730

Cited by 34 publications

(19 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This feature can be used to reduce the viscosity of heavy oil, which is rich in asphaltenes and gums, and its high viscosity and poor fluidity cause great difficulties in oil exploitation [ 36 ]. Surfactin can emulsify strongly hydrophobic substrates such as pentadecane, diesel, hexane and kerosene [ 37 – 40 ]. A comparison of the emulsification activity of the two surfactin preparations at 200 mg/L is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Genetic engineering of the precursor supply pathway for the overproduction of the nC14-surfactin isoform with promising MEOR applications

Cai

Lin

et al. 2021

Microb Cell Fact

View full text Add to dashboard Cite

Background Surfactin, a representative biosurfactant of lipopeptide mainly produced by Bacillus subtilis, consists of a cyclic heptapeptide linked to a β-hydroxy fatty acid chain. The functional activity of surfactin is closely related to the length and isomerism of the fatty acid chain. Results In this study, the fatty acid precursor supply pathway in Bacillus subtilis 168 for surfactin production was strengthened through two steps. Firstly, pathways competing for the precursors were eliminated with inactivation of pps and pks. Secondly, the plant medium-chain acyl-carrier protein (ACP) thioesterase (BTE) from Umbellularia californica was overexpressed. As a result, the surfactin titer after 24 h of cultivation improved by 34%, and the production rate increased from 0.112 to 0.177 g/L/h. The isoforms identified by RP-HPLC and GC–MS showed that the proportion of nC14-surfactin increased 6.4 times compared to the control strain. A comparison of further properties revealed that the product with more nC14-surfactin had higher surface activity and better performance in oil-washing. Finally, the product with more nC14-surfactin isoform had a higher hydrocarbon-emulsification index, and it increased the water-wettability of the oil-saturated silicate surface. Conclusion The obtained results identified that enhancing the supply of fatty acid precursor is very essential for the synthesis of surfactin. At the same time, this study also proved that thioesterase BTE can promote the production of nC14-surfactin and experimentally demonstrated its higher surface activity and better performance in oil-washing. These results are of great significance for the MEOR application of surfactin. Graphic abstract

show abstract

Section: Resultsmentioning

confidence: 99%

Genetic engineering of the precursor supply pathway for the overproduction of the nC14-surfactin isoform with promising MEOR applications

Cai

Lin

et al. 2021

Microb Cell Fact

View full text Add to dashboard Cite

show abstract

“…Thus, they display as physico-chemical properties foaming (Razafindralambo et al, 1998 ; Fei et al, 2020 ), emulsifying (Deleu et al, 1999 ; Liu et al, 2015 ; Long et al, 2017 ; Fei et al, 2020 ) and dispersing properties, solid surface wetting and surface hydrophobicity modification performance (Ahimou et al, 2000 ; Shakerifard et al, 2009 ; Marcelino et al, 2019 ; Fei et al, 2020 ), and chelating ability (Mulligan et al, 1999 ; Grangemard et al, 2001 ; Eivazihollagh et al, 2019 ). This strong surface activity leads to detergent applications (Zezzi do Valle Gomes and Nitschke, 2012 ), but they also show promising perspectives of applications in the environmental sector to enhance oil recovery in oil-producing wells (Liu et al, 2015 ; Joshi et al, 2016 ; Long et al, 2017 ; de Araujo et al, 2019 ; Alvarez et al, 2020 ; Miyazaki et al, 2020 ), to increase the biodegradation rate of linear and aromatic hydrocarbons (Wang et al, 2020 ), and for metal removal from soil or aqueous solutions (Zouboulis et al, 2003 ; Eivazihollagh et al, 2019 ). Very recently, it was also suggested that surfactin can effectively demulsify waste crude oil (Yang et al, 2020 ).…”

Section: Structure and Properties Relationshipmentioning

confidence: 99%

The Surfactin-Like Lipopeptides From Bacillus spp.: Natural Biodiversity and Synthetic Biology for a Broader Application Range

Théatre

Cano-Prieto

Bartolini

et al. 2021

Front. Bioeng. Biotechnol.

113

View full text Add to dashboard Cite

Surfactin is a lipoheptapeptide produced by several Bacillus species and identified for the first time in 1969. At first, the biosynthesis of this remarkable biosurfactant was described in this review. The peptide moiety of the surfactin is synthesized using huge multienzymatic proteins called NonRibosomal Peptide Synthetases. This mechanism is responsible for the peptide biodiversity of the members of the surfactin family. In addition, on the fatty acid side, fifteen different isoforms (from C12 to C17) can be incorporated so increasing the number of the surfactin-like biomolecules. The review also highlights the last development in metabolic modeling and engineering and in synthetic biology to direct surfactin biosynthesis but also to generate novel derivatives. This large set of different biomolecules leads to a broad spectrum of physico-chemical properties and biological activities. The last parts of the review summarized the numerous studies related to the production processes optimization as well as the approaches developed to increase the surfactin productivity of Bacillus cells taking into account the different steps of its biosynthesis from gene transcription to surfactin degradation in the culture medium.

show abstract

“…This feature can be used to reduce the viscosity of heavy oil, which is rich in asphaltenes and gums, and its high viscosity and poor uidity cause great di culties in oil exploitation [36]. Surfactin can emulsify strongly hydrophobic substrates such as pentadecane, diesel, hexane and kerosene [37][38][39][40]. A comparison of the emulsi cation activity of the two surfactin preparations at 200 mg/L is shown in Fig.…”

Section: Hplc-ms Analysis Of the Isomeric Composition Of Surfactinmentioning

confidence: 99%

Overexpression of the Plant Medium-chain Acyl-carrier Protein Thioesterase BTE for the Overproduction of the nC14-surfactin Isoform with Promising MEOR Applications

Cai

Lin

et al. 2021

Preprint

View full text Add to dashboard Cite

BackgroundSurfactin, a representative biosurfactant of popeptide mainly produced by Bacillus subtilis, consists of a cyclic heptapeptide linked to a β-hydroxy fatty acid chain. The functional activity of surfactin is closely related to the length and isomerism of the fatty acid chain. ResultsIn this study, the plant medium-chain acyl-carrier protein (ACP) thioesterase (BTE) from Umbellularia californica was overexpressed in a recombinant surfactin production strain based on B. subtilis 168. As a result, the surfactin yield after 24 h of cultivation improved by 23%, and the production rate increased from 0.112 to 0.177 g/L/h. The isoforms identified by RP-HPLC and GC-MS showed that the proportion of nC14-surfactin increased 6.4 times compared to the control strain. A comparison of further properties revealed that the product with more nC14-surfactin had higher surface activity and better performance in oil-washing. Finally, the product with more nC14-surfactin isoform had a higher hydrocarbon-emulsification index, and it increased the water-wettability of the oil-saturated silicate surface. ConclusionThe obtained results provide an original approach to modify the fatty acid chain of surfactin and further demonstrate the importance of the length and isomerism of the β-hydroxy fatty acid chain for the MEOR application of surfactin.

show abstract

Microbial enhanced oil recovery potential of surfactin-producing Bacillus subtilis AB2.0

Cited by 34 publications

References 42 publications

Genetic engineering of the precursor supply pathway for the overproduction of the nC14-surfactin isoform with promising MEOR applications

Genetic engineering of the precursor supply pathway for the overproduction of the nC14-surfactin isoform with promising MEOR applications

The Surfactin-Like Lipopeptides From Bacillus spp.: Natural Biodiversity and Synthetic Biology for a Broader Application Range

Overexpression of the Plant Medium-chain Acyl-carrier Protein Thioesterase BTE for the Overproduction of the nC14-surfactin Isoform with Promising MEOR Applications

Contact Info

Product

Resources

About