Microbial Diversity Analysis and Genome Sequencing Identify Xanthomonas perforans as the Pathogen of Bacterial Leaf Canker of Water Spinach (Ipomoea aquatic)

Hu, Ming; Li, Chuhao; Zhou, Xin; Yang, Xue; Wang, Si; Hu, Anqun; Chen, Shanshan; Mo, Xiuwen; Zhou, Jianuan

doi:10.3389/fmicb.2021.752760

Cited by 9 publications

(6 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On 13th October 2019, four corms of taro plants showing wilting leaves were collected from Laojun Village, Ruyuan County, Shaoguan City, Guangdong Province, China. Microbial separation from the symptomatic taro was carried out using the method previously described ( Hu et al, 2021b ). Firstly, the corms were washed with tap water and then surface-disinfected with 70% ethanol solution.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Genome Analysis of Pectobacterium colocasium sp. nov. and Pectobacterium aroidearum, Two New Pathogens of Taro

Zhou

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Bacterial soft rot is one of the most destructive diseases of taro (Colocasia esculenta) worldwide. In recent years, frequent outbreaks of soft rot disease have seriously affected taro production and became a major constraint to the development of taro planting in China. However, little is known about the causal agents of this disease, and the only reported pathogens are two Dickeya species and P. carotovorum. In this study, we report taro soft rot caused by two novel Pectobacterium strains, LJ1 and LJ2, isolated from taro corms in Ruyuan County, Shaoguan City, Guangdong Province, China. We showed that LJ1 and LJ2 fulfill Koch’s postulates for taro soft rot. The two pathogens can infect taro both individually and simultaneously, and neither synergistic nor antagonistic interaction was observed between the two pathogens. Genome sequencing of the two strains indicated that LJ1 represents a novel species of the genus Pectobacterium, for which the name “Pectobacterium colocasium sp. nov.” is proposed, while LJ2 belongs to Pectobacterium aroidearum. Pan-genome analysis revealed multiple pathogenicity-related differences between LJ1, LJ2, and other Pectobacterium species, including unique virulence factors, variation in the copy number and organization of Type III, IV, and VI secretion systems, and differential production of plant cell wall degrading enzymes. This study identifies two new soft rot Pectobacteriaceae (SRP) pathogens causing taro soft rot in China, reports a new case of co-infection of plant pathogens, and provides valuable resources for further investigation of the pathogenic mechanisms of SRP.

show abstract

Section: Methodsmentioning

confidence: 99%

Isolation and Genome Analysis of Pectobacterium colocasium sp. nov. and Pectobacterium aroidearum, Two New Pathogens of Taro

Zhou

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…D. zeae MS2 isolated from banana in Guangdong Province, China [ 5 , 36 ] and Dickeya strain FZ06 isolated from Philippine banana were used in this study. The strains were grown in LB medium (typtone 10.0 g/L, yeast extract powder 5.0 g/L, NaCl 10.0 g/L, pH 7.0, 1.5 g/L agar added in solid medium) at 28 °C [ 77 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Pathogenomic Analysis of Two Banana Pathogenic Dickeya Strains Isolated from China and the Philippines

Tan

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Dickeya is a major and typical member of soft rot Pectobacteriaceae (SRP) with a wide range of plant hosts worldwide. Previous studies have identified D. zeae as the causal agent of banana soft rot disease in China. In 2017, we obtained banana soft rot pathogen strain FZ06 from the Philippines. Genome sequencing and analysis indicated that FZ06 can be classified as D. dadantii and represents a novel subspecies of D. dadantii, which we propose to name as subsp. paradisiaca. Compared with Chinese banana soft rot pathogenic strain D. zeae MS2, strain FZ06 has a similar host range but different virulence; FZ06 is significantly less virulent to banana and potato but more virulent to Chinese cabbage and onion. Characterization of virulence factors revealed obviously less production of pectate lyases (Pels), polygalacturonases (Pehs), proteases (Prts), and extrapolysaccharides (EPSs), as well as lower swimming and swarming motility and biofilm formation in strain FZ06. Genomic comparison of the two strains revealed five extra gene clusters in FZ06, including one Stt-type T2SS, three T4SSs, and one T4P. Expression of cell wall degrading enzyme (CWDE)-encoding genes is significantly lower in FZ06 than in MS2.

show abstract

“…All related genes in conjugative transfer systems of plasmids were predicted by a thorough homologous search with genes in the oriTDB ( Li X. et al, 2018 ) and SecRet4 databases ( Bi et al, 2013 ; Hu et al, 2021 ). Relaxases, T4CPs and MPFs/T4SS genes in plasmids were predicted with identity > = 50, coverage > = 50% and e-value <=0.001.…”

Section: Methodsmentioning

confidence: 99%

Comparative genomics reveals new insights into the evolution of the IncA and IncC family of plasmids

Zhang

Yin

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Incompatibility groups IncA and IncC plasmids are of great concern due to their ability to disseminate antibiotic resistance in bacteria via conjugative transfer. A deep understanding of their genomic structures and evolutionary characteristics is of great significance for improving our knowledge about its multidrug-resistance evolution and dissemination. However, current knowledge of their backbone structure, features of core functional modules and the characteristics of variable regions is based on a few plasmids, which highlights the need for a comprehensive systematic study. The present study thoroughly compared and analysed 678 IncA and IncC plasmid genomes. We found that their core functional genes were occasionally deficient and sometimes existed as multiple functional copies/multiple families, which resulted in much diversity. The phylogeny of 13 core functional genes corresponded well to the plasmid subtypes. The conjugative transfer system gained diverse complexity and exhibited many previously unnoticed types with multiple combinations. The insertion of mobile genetic elements (MGEs) in plasmids varied between types and was present in 4 insertion spots in different types of plasmids with certain types of transposons, integrons and insertion sequences. The impact of gene duplication, deletion, the insertion of MGEs, genome rearrangement and recombination resulted in the complex dynamic variable backbone of IncA and IncC plasmids. And IncA and IncC plasmids were more complex than their closest relative SXT/R391 integrative conjugative elements (ICEs), which included nearly all of the diversity of SXT/R391 in key systems. Our work demonstrated a global and systematic view of the IncA and IncC plasmids and provides many new insights into their genome evolution.

show abstract

Microbial Diversity Analysis and Genome Sequencing Identify Xanthomonas perforans as the Pathogen of Bacterial Leaf Canker of Water Spinach (Ipomoea aquatic)

Cited by 9 publications

References 54 publications

Isolation and Genome Analysis of Pectobacterium colocasium sp. nov. and Pectobacterium aroidearum, Two New Pathogens of Taro

Isolation and Genome Analysis of Pectobacterium colocasium sp. nov. and Pectobacterium aroidearum, Two New Pathogens of Taro

Comparative Pathogenomic Analysis of Two Banana Pathogenic Dickeya Strains Isolated from China and the Philippines

Comparative genomics reveals new insights into the evolution of the IncA and IncC family of plasmids

Contact Info

Product

Resources

About