Microbial-derived glycolipids in the sustainable formulation of biomedical and personal care products: A consideration of the process economics towards commercialization

Onwosi, Chukwudi O.; Aliyu, Godwin O.; Onu, Chisom; Chukwu, Kenechi O.; Ndukwe, Johnson K.; Igbokwe, Victor C.

doi:10.1016/j.procbio.2020.10.001

Cited by 17 publications

(12 citation statements)

References 142 publications

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“…In general, biosurfactant molecules can be applied in various area such as the nutrient, cosmetic, textile, varnish, mining, oil recovery and pharmaceutical industries, as showing lower toxicity and improved physicochemical and functional properties in comparison to their synthetic analogues [50][51][52][53][54]. However, one of the main barriers to the biotechnological development of trehalolipids is due to the fact that they are often cell-bound, thus hampering the large-scale production and purification due to high downstream costs [13].…”

Section: Discussionmentioning

confidence: 99%

Combining OSMAC Approach and Untargeted Metabolomics for the Identification of New Glycolipids with Potent Antiviral Activity Produced by a Marine Rhodococcus

Esposito

Giugliano

Sala

et al. 2021

IJMS

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Natural products of microbial origin have inspired most of the commercial pharmaceuticals, especially those from Actinobacteria. However, the redundancy of molecules in the discovery process represents a serious issue. The untargeted approach, One Strain Many Compounds (OSMAC), is one of the most promising strategies to induce the expression of silent genes, especially when combined with genome mining and advanced metabolomics analysis. In this work, the whole genome of the marine isolate Rhodococcus sp. I2R was sequenced and analyzed by antiSMASH for the identification of biosynthetic gene clusters. The strain was cultivated in 22 different growth media and the generated extracts were subjected to metabolomic analysis and functional screening. Notably, only a single growth condition induced the production of unique compounds, which were partially purified and structurally characterized by liquid chromatography high-resolution tandem mass spectrometry (LC-HRMS/MS). This strategy led to identifying a bioactive fraction containing >30 new glycolipids holding unusual functional groups. The active fraction showed a potent antiviral effect against enveloped viruses, such as herpes simplex virus and human coronaviruses, and high antiproliferative activity in PC3 prostate cancer cell line. The identified compounds belong to the biosurfactants class, amphiphilic molecules, which play a crucial role in the biotech and biomedical industry.

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Section: Discussionmentioning

confidence: 99%

Combining OSMAC Approach and Untargeted Metabolomics for the Identification of New Glycolipids with Potent Antiviral Activity Produced by a Marine Rhodococcus

Esposito

Giugliano

Sala

et al. 2021

IJMS

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“…Methodologies are constantly being improved to screen high numbers of isolates and correlate the findings with available data [ 169 , 170 ], as well as, for screening clones from metagenomic libraries [ 159 ]. Molecular techniques could be applied to screen strains with single gene clusters and islands of inherent production capacity, which would also facilitate the process and identification of similar gene sequences in other strains [ 171 ].…”

Section: Screening Of Biosurfactant-producing Fungi: Methodologies and Characterizationmentioning

confidence: 99%

“…However, the regulatory mechanisms that determine genomic analysis and attempts to induce the overproduction of biosurfactants can only be explored in a restricted way due to the wide structural variety of these molecules and biosynthesis pathways involved in production [ 156 ]. The application of advanced tools in genomics, transcriptomics, proteomics and metabolomics to elucidate complete biosynthetic pathways and their regulation in biosurfactant-producing strains, improves our understanding of the bottle necks in synthesis pathways of some metabolites, diverting most of the energy to the main metabolic pathways involved in the production of biosurfactant [ 171 ].…”

Section: New Production Technologiesmentioning

confidence: 99%

Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications

Silva

Banat

Giachini

et al. 2021

Bioprocess Biosyst Eng

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Biosurfactants are in demand by the global market as natural commodities that can be added to commercial products or use in environmental applications. These biomolecules reduce the surface/interfacial tension between fluid phases and exhibit superior stability to chemical surfactants under different physico-chemical conditions. Biotechnological production of biosurfactants is still emerging. Fungi are promising producers of these molecules with unique chemical structures, such as sophorolipids, mannosylerythritol lipids, cellobiose lipids, xylolipids, polyol lipids and hydrophobins. In this review, we aimed to contextualize concepts related to fungal biosurfactant production and its application in industry and the environment. Concepts related to the thermodynamic and physico-chemical properties of biosurfactants are presented, which allows detailed analysis of their structural and application. Promising niches for isolating biosurfactant-producing fungi are presented, as well as screening methodologies are discussed. Finally, strategies related to process parameters and variables, simultaneous production, process optimization through statistical and genetic tools, downstream processing and some aspects of commercial products formulations are presented.

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“…Glycolipids are found in membranes of most living organisms and their carbohydrate components are directly involved with their recognition/protection properties [ 17 , 18 ]. Microbial-derived glycolipids are increasingly serving as models for sustainable and stable sources of highly diversified, yet simple and economically viable glycolipids [ 19 ]. Together with other glycoconjugate members of cell surface glycoproteins, they form the glycocalyx, which coats the cells that provide contact with their environment.…”

Section: Natural Glycolipidsmentioning

confidence: 99%

Recent Development in the Design of Neoglycoliposomes Bearing Arborescent Architectures

2021

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This brief review highlights systematic progress in the design of synthetic glycolipid (neoglycolipids) analogs evolving from the conventional architectures of natural glycosphingolipids and gangliosides. Given that naturally occurring glycolipids are composed of only one hydrophilic sugar head-group and two hydrophobic lipid tails embedded in the lipid bilayers of the cell membranes, they usually require extraneous lipids (phosphatidylcholine, cholesterol) to confer their stability. In order to obviate the necessity for these additional stabilizing ingredients, recent investigations have merged dendrimer chemistry with that of neoglycolipid syntheses. This singular approach has provided novel glycoarchitectures allowing reconsidering the necessity for the traditional one to two hydrophilic/hydrophobic ratio. An emphasis has been provided in the recent design of modular arborescent neoglycolipid syntheses coined glycodendrimersomes.

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Microbial-derived glycolipids in the sustainable formulation of biomedical and personal care products: A consideration of the process economics towards commercialization

Cited by 17 publications

References 142 publications

Combining OSMAC Approach and Untargeted Metabolomics for the Identification of New Glycolipids with Potent Antiviral Activity Produced by a Marine Rhodococcus

Combining OSMAC Approach and Untargeted Metabolomics for the Identification of New Glycolipids with Potent Antiviral Activity Produced by a Marine Rhodococcus

Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications

Recent Development in the Design of Neoglycoliposomes Bearing Arborescent Architectures

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