Microbial degradation of n-hexadecane in mineral salt medium as mediated by degradative enzymes

Mishra, Shweta; Singh, Sarita

doi:10.1016/j.biortech.2012.02.049

Cited by 89 publications

(52 citation statements)

References 42 publications

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“…The produced aldehyde was metabolized towards corresponding carboxylic acid by the produced ADHs in a BH broth. This carboxylic acid, mainly serve as a substrate for alkane degrading microbes to accumulate fatty acids by b-oxidation pathway (May and Katapodis 1990;Lal and Khanna 1996;Mishra and Singh 2012). Thus, the roles of ADHs were crucial to determine the degradability of the n-alkanes and support the growth of degrading microbes in the BH broth.…”

Section: Discussionmentioning

confidence: 99%

“…The AHy experimental methodology was adopted as previously described by Mishra and Singh (2012). In Brief, the cell-free supernatant was collected by centrifugation at 8000 rpm at 10 min (Remi, C-24BL, India) and used for AHy assay.…”

Section: Alkane Hydroxylase Assaymentioning

confidence: 99%

“…One unit of lipase activity (U) was calculated as lM of fatty acid released per min. Lipase activity was expressed in unit per mL, i.e., U mL -1 (Mishra and Singh 2012).…”

Section: Determination Of Extracellular Lipasementioning

confidence: 99%

“…Cell biomass were harvested by centrifugation at 8000 g for 10 min in 4°C, washed twice with PUM buffer (Mishra and Singh 2012), and re-suspended in PUM buffer to maintain initial absorbance (at OD 400 ) values between 0.12 and 0.14. Around 1 mL of hexadecane and 2 mL of the adjusted cell suspension were vortexed at high speed for 2 min and incubated at 30°C for 15 min to allow the phase separation of hydrocarbons in floating layer.…”

Section: Bacterial Adhesion To Hydrocarbons (Bath)mentioning

confidence: 99%

“…While, similar trend was observed for lipase activity when the medium was supplied with C 40 , i.e., IR2 showed (37 lmol mg -1 protein), IR4 (41 lmol mg -1 protein), MN6 (39 lmol mg -1 protein) and mixed consortium (57 lmol mg -1 protein). Cell surface properties, changing composition of the cellular fatty acids in the presence of various hydrocarbons were reported to improve the lipophilicity and could assist the uptake, absorption and import of the hydrocarbons (Mishra and Singh 2012).…”

Section: Lipase Assaymentioning

confidence: 99%

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Enzyme-mediated biodegradation of long-chain n-alkanes (C32 and C40) by thermophilic bacteria

et al. 2017

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Removal of long-chain hydrocarbons and nalkanes from oil-contaminated environments are mere important to reduce the ecological damages, while bioaugmentation is a very promising technology that requires highly efficient microbes. In present study, the efficiency of pure isolates, i.e., Geobacillus thermoparaffinivorans IR2, Geobacillus stearothermophillus IR4 and Bacillus licheniformis MN6 and mixed consortium on degradation of long-chain n-alkanes C 32 and C 40 was investigated by batch cultivation test. Biodegradation efficiencies were found high for C 32 by mixed consortium (90%) than pure strains, while the pure strains were better in degradation of C 40 than mixed consortium (87%). In contrast, the maximum alkane hydroxylase activities (161 lmol mg -1 protein) were recorded in mixed consortium system that had supplied with C 40 as sole carbon source. Also, the alcohol dehydrogenase (71 lmol mg -1 protein) and lipase activity (57 lmol mg -1 protein) were found high. Along with the enzyme activities, the hydrophobicity natures of the bacterial strains were found to determine the degradation efficiency of the hydrocarbons. Thus, the study suggested that the hydrophobicity of the bacteria is a critical parameter to understand the biodegradation of n-alkanes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Alkane Hydroxylase Assaymentioning

confidence: 99%

“…One unit of lipase activity (U) was calculated as lM of fatty acid released per min. Lipase activity was expressed in unit per mL, i.e., U mL -1 (Mishra and Singh 2012).…”

Section: Determination Of Extracellular Lipasementioning

confidence: 99%

Section: Bacterial Adhesion To Hydrocarbons (Bath)mentioning

confidence: 99%

Section: Lipase Assaymentioning

confidence: 99%

See 3 more Smart Citations

Enzyme-mediated biodegradation of long-chain n-alkanes (C32 and C40) by thermophilic bacteria

et al. 2017

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Biosurfactant Production Using Bioreactors from Industrial Byproducts

Karnwal

2021

Biosurfactants for a Sustainable Future

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Grasses andRhodococcuserythropolisBacteria for Bioremediation of Naturally Polluted Soils with Hydrocarbons

Mirzakhani

Nejad

2016

Chem Eng & Technol

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A combination of grasses, Kentucky bluegrass and Bermuda grass, with Rhodococcus erythropolis bacteria was hypothesized for bioremediation of naturally polluted soils with hydrocarbons. A 90‐day greenhouse experiment as a factorial design with twelve treatments including soil inoculation with bacteria as well as soil sterilization using both plants in three replicates was performed. Both grasses significantly decreased the concentration of soil hydrocarbons compared with the control polluted soil, with Kentucky grass being the more effective one. The bacteria enhanced the bioremediation abilities of both plants, especially of Kentucky grass. Soil sterilization substantially reduced the rate of hydrocarbons in the soil. The positive effects of bacteria on the process of bioaugmentation make this method of bioremediation strongly recommendable.

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Microbial degradation of n-hexadecane in mineral salt medium as mediated by degradative enzymes

Cited by 89 publications

References 42 publications

Enzyme-mediated biodegradation of long-chain n-alkanes (C32 and C40) by thermophilic bacteria

Enzyme-mediated biodegradation of long-chain n-alkanes (C32 and C40) by thermophilic bacteria

Biosurfactant Production Using Bioreactors from Industrial Byproducts

Grasses andRhodococcuserythropolisBacteria for Bioremediation of Naturally Polluted Soils with Hydrocarbons

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