Microbial degradation of microcystin in Florida’s freshwaters

Ramani, A.; Rein, Kathleen S.; Shetty, Kateel G.; Jayachandran, Krish

doi:10.1007/s10532-011-9484-y

Cited by 57 publications

(32 citation statements)

References 32 publications

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“…3a and b). Only a few genera capable of degrading MCLR fall into these two phyla (Liu et al, 2007;Manage et al, 2009;Alamri et al, 2010;Ramani et al, 2012), but these genera were all detected at low abundance here, and further declined or even disappeared on day 7 (Table 3). A probable reason for this phenomenon was that these genera suffered from competition against the strongly growing Proteobacteria population, which might compromised the participation of these genera in MCLR-biodegradation process in sediment.…”

Section: Shifts Of Composition Profile In Response To Sediment-mediatmentioning

confidence: 68%

Discerning biodegradation and adsorption of microcystin-LR in a shallow semi-enclosed bay and bacterial community shifts in response to associated process

Shi

et al. 2016

Ecotoxicology and Environmental Safety

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Section: Shifts Of Composition Profile In Response To Sediment-mediatmentioning

confidence: 68%

Discerning biodegradation and adsorption of microcystin-LR in a shallow semi-enclosed bay and bacterial community shifts in response to associated process

Shi

et al. 2016

Ecotoxicology and Environmental Safety

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“…and Sphingomonas sp. have been reported to be capable of utilizing and degrading microcystin [25]. Hydrogenophaga has been reported to be an algicidal bacterium against harmful algae bloom [9].…”

Section: Resultsmentioning

confidence: 99%

Microcystis aeruginosagrown in different defined media leads to different cultivable heterotrophic bacteria composition that could influence cyanobacterial morphological characteristics and growth properties

Khong

Khaw

Nazarudin

et al. 2019

Preprint

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22Cyanobacterial blooms involving Microcystis spp. often pose severe problems to the 23 environment and general community due to their persistent presence in eutrophic water 24 bodies and potential to form blooms. Bacterial associations are known to alter 25 microenvironment of Microcystis and potentially influence their development. This study 26 aimed to study cultivable heterotrophic bacteria composition that developed symbiotically 27 with Microcystis aeruginosa naturally as well as those cultured under defined media and their 28 possible effects on the morphology and growth properties of the cyanobacterium. M. 29 aeruginosa (UPMC-A0051) was isolated during a bloom from Putrajaya Lake, Malaysia and 30 characterized as a non microcystin-producing cyanobacterium using PCR and 31 chromatographic methods. Associated heterotrophic bacteria were then isolated and identified 32 from the culture media as well as the lake where the cyanobacterium was originally isolated. 33A total of 16 bacterial species were isolated from the lake and none of them were similar to 34 the bacteria associated with M. aeruginosa cultured in artificial media. Cultivable 35 heterotrophic bacteria composition associated with M. aeruginosa were also distinct in 36 different culture media, despite the same inoculum. These bacteria were classified under 37 Actinobacteria, α.-Proteobacteria and β-Proteobacteria. Under different bacterial 38 associations, M. aeruginosa cultivated in defined media showed different colony morphology 39 and growth properties. The present study demonstrated that distinct bacterial composition 40 observed in different culture media could be responsible for dissimilar cyanobacterium 41 morphology and growth rate, particularly on the clustering pattern. In the axenic culture, the 42 growth of M. aeruginosa was significantly reduced indicating the influence of associated 43 bacteria on the development of cyanobacterial colonies. 44 45 Keywords: Cyanobacteria; Microcystis; non-microcystin production; heterotrophic 46 bacteria; morphology; growth; bacterial-microalgae interaction; axenic culture 47 48 Episodes of cyanobacterial blooms have been reported unceasingly worldwide over the past 49 century due to increased unsustainable anthropogenic activities resulting in related incidences 50 of water enrichment and eutrophication. The blooms affect fishery resources, recreational 51 activities, domestic water usage and aesthetic values of aquatic ecosystems. Previous studies 52 of cyanobacterial water blooms have focused on cyanobacterial species and various factors in 53 influencing the cyanobacterial growth [1]. 54 Microcystis frequently forms dense surface blooms in freshwater environments 55 leading to serious water quality problems. It has been established that cyanobacterial blooms 56 in temperate waters are significantly different from those in tropical waters [2]. At such, algae 57 reinvasion is a major interest in the study of temperate Microcystis blooms which have been 58 reported to be influenced by envi...

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“…Microbial biodegradation is an environmentally-friendly and effective treatment method for detoxify MC-LR in natural waters without potential harmful by-products. Some MC-degrading pure bacterial strains have been isolated and have their MC-LR-degrading rates reported [6, 16, 17, 19]. For example, the single pure bacterial strain Sphingomonas sp.…”

Section: Discussionmentioning

confidence: 99%

“…Several previous investigations demonstrated that microbial biodegradation may be one of the most environmentally-friendly, effective and promising treatment methods for removing MC-LR in natural waters, since it can detoxify MC-LR and don’t generated any apparent potential harmful by-products [3, 6, 14-17]. A few MC-degrading pure bacterial strains have been isolated, identified and had their mechanisms reported, and most of the isolated MC-degrading bacteria were limited to the family Sphingomonadaceae [16-18]. In practice, native bacterial communities (indigenous bacterial mixed culture) may be more suitable for degrading MC-LR in the environment compared to the single pure bacterial strains [6,19].…”

Section: Introductionmentioning

confidence: 99%

Aerobic removal of microcystin-LR by a novel native effective bacterial community designated as YFMCD4 isolated from Lake Taihu

Guo

Huang

Massey

et al. 2018

Preprint

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12 Microcystins (MCs) are a group of monocyclic heptapeptide hepatotoxins produced by species of 13 cyanobacteria. MC-LR is the most toxic and frequently detected MCs variant in water, which 14 poses a great threat to the natural ecosystem and public health. It's important to seek 15 environment-friendly and cost-efficient methods to remove MC-LR. To investigate the MC-16 degrading capacities of a novel indigenous bacterial community designated as YFMCD4 and the 17 influence of environmental factors including various temperatures, MC concentrations and pH on 18 the MC-degrading activities, the concentration of MC-LR was measured by high performance 19liquid chromatography. In addition, the MC-degrading mechanism containing the degradation 20 pathway and products of YFMCD4 was studied using HPLC coupled with an ultra-high 21 resolution LTQ Orbitrap Velos Pro ETD mass spectrometry equipped with electrospray 22 ionization interface. The data showed MC-LR can be removed at the maximum rate of 0.5 23 µg/(ml·h) by YFMCD4 containing Alcaligenes faecalis and Stenotrophomonas acidaminiohila. 24The MC-degrading rates of YFMCD4 were significantly affected by different temperatures, pH 25 and MC-LR concentrations. Two intermediates of a tetrapeptide and Adda appeared in the 26 degradation process. These results illustrate that the novel bacterial community YFMCD4 can 27 remove MC-LR effectively and completely, which indicates YFMCD4 possesses a significant 28 potential to be used in bioremediation of water bodies contaminated by MC-LR. 29 3 47 MC-LR is very stable and resistant to many natural factors including extreme pH, high 48 temperature and sunlight in the environment owing to the cyclic structure [3, 6, 12]. Moreover, 49 MC-LR can be accumulated in aquatic organisms and food crops representing a health hazard to 50 human and animals through food chains [13]. It is very important to reduce MC-LR 51 concentration in freshwater ecosystem. However, conventional drinking water treatments have 52 limited efficacy in removing MC-LR. Some physical and chemical methods containing 53 ozonation, chlorination, photocatalysis and electrolysis have been proposed for MC-LR 54 elimination from drinking water. However, all these methods have certain limitations in terms of 55 high operating costs, low efficacy and harmful by-products [3, 6, 14, 15]. It's desirable that 56investigators seek other environmentally-benign and cost-efficient methods and technologies to 57 remove MC-LR found in water bodies [3, 6,[14][15][16][17]. 58Several previous investigations demonstrated that microbial biodegradation may be one of 59 the most environmentally-friendly, effective and promising treatment methods for removing 60 MC-LR in natural waters, since it can detoxify MC-LR and don't generated any apparent 61 potential harmful by-products [3, 6,[14][15][16][17]. A few MC-degrading pure bacterial strains have 62 been isolated, identified and had their mechanisms reported, and most of the isolated MC-63 degrading bacteria were limited to the family S...

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Microbial degradation of microcystin in Florida’s freshwaters

Cited by 57 publications

References 32 publications

Discerning biodegradation and adsorption of microcystin-LR in a shallow semi-enclosed bay and bacterial community shifts in response to associated process

Discerning biodegradation and adsorption of microcystin-LR in a shallow semi-enclosed bay and bacterial community shifts in response to associated process

Microcystis aeruginosagrown in different defined media leads to different cultivable heterotrophic bacteria composition that could influence cyanobacterial morphological characteristics and growth properties

Aerobic removal of microcystin-LR by a novel native effective bacterial community designated as YFMCD4 isolated from Lake Taihu

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