Microbial copper resistance: importance in biohydrometallurgy

Martínez-Bussenius, Cristóbal; Navarro, C.; Jerez, Carlos A.

doi:10.1111/1751-7915.12450

Cited by 62 publications

(38 citation statements)

References 105 publications

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“…Acidophilic bacteria used in biomining processes have developed diverse strategies to cope with high metal ions concentrations, including active mechanisms also present in neutrophilic microorganisms, as well as passive mechanisms, such as complexation of metal ions with sulfate (Baillet et al, 1997 ; Dopson and Holmes, 2014 ). In addition, resistance to heavy metals like copper and cadmium has been related to the synthesis/degradation of inorganic phosphate granules (polyphosphate, or polyP) in Acidithiobacillus ferrooxidans (Dopson et al, 2003 ; Alvarez and Jerez, 2004 ; Martínez-Bussenius et al, 2017 ). In this model, when bacterial cells are grown in presence of high metal concentrations, there is a decrease in polyP levels and a concomitant formation of metal-phosphate complexes that contribute to detoxification (Dopson et al, 2003 ; Alvarez and Jerez, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidans ATCC 19703 by Improving Metal Uptake and Tolerance

et al. 2018

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Recently, we reported the production of Cadmium sulfide (CdS) fluorescent semiconductor nanoparticles (quantum dots, QDs) by acidophilic bacteria of the Acidithiobacillus genus. Here, we report that the addition of inorganic phosphate to Acidithiobacillus thiooxidans ATCC 19703 cultures favors the biosynthesis of CdS QDs at acidic conditions (pH 3.5). The effect of pH, phosphate and cadmium concentrations on QDs biosynthesis was studied by using Response Surface Methodology (RSM), a multivariate technique for analytical optimization scarcely used in microbiological studies to date. To address how phosphate affects intracellular biosynthesis of CdS QDs, the effect of inorganic phosphate on bacterial cadmium-uptake was evaluated. By measuring intracellular levels of cadmium we determined that phosphate influences the capacity of cells to incorporate this metal. A relation between cadmium tolerance and phosphate concentrations was also determined, suggesting that phosphate participates in the adaptation of bacteria to toxic levels of this metal. In addition, QDs-biosynthesis was also favored by the degradation of intracellular polyphosphates. Altogether, our results indicate that phosphate contributes to A. thiooxidans CdS QDs biosynthesis by influencing cadmium uptake and cadmium tolerance. These QDs may also be acting as a nucleation point for QDs formation at acidic pH. This is the first study reporting the effect of phosphates on QDs biosynthesis and describes a new cadmium-response pathway present in A. thiooxidans and most probably in other bacterial species.

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Section: Introductionmentioning

confidence: 99%

Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidans ATCC 19703 by Improving Metal Uptake and Tolerance

et al. 2018

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“…By contrast, while PPX homologs were identified within the Crenarchaeota, concentrated in the order Sulfolobales, no PPK1 or 2 homologs were retrieved from this taxon. Martinez-Bussenius et al 53 has previously shown the presence of ppx but not ppk1 in 6 species of Sulfolobus sp., and Metallosphaera sedula.…”

Section: Discussionmentioning

confidence: 97%

The potential for polyphosphate metabolism in Archaea and anaerobic polyphosphate formation inMethanosarcina mazei

Paula

Chin

Schnürer

et al. 2019

Preprint

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2Inorganic polyphosphate (polyP) is ubiquitous across all forms of life, but the study of its metabolism has been mainly confined to bacteria and yeasts. Few reports detail the presence and accumulation of polyP in Archaea, and little information is available on its functions and regulation. Here, we report that homologs of bacterial polyP metabolism proteins are present across the major taxa in the Archaea, suggesting that archaeal populations may have a greater contribution to global phosphorus cycling than has previously been recognised. We also demonstrate that polyP accumulation can be induced under strictly anaerobic conditions, in response to changes in phosphate (Pi) availability, i.e. Pi starvation, followed by incubation in Pi replete media (overplus), in cells of the methanogenic archaeon Methanosarcina mazei.Pi-starved M. mazei cells increased transcript abundance of the PHO-regulated alkaline phosphatase (phoA) gene and of the high-affinity phosphate transport (pstSCAB-phoU) operon: no increase in polyphosphate kinase 1 (ppk1) transcript abundance was observed.Subsequent incubation of Pi-starved M. mazei cells under Pi replete conditions, led to a 237% increase in intracellular polyphosphate content and a >5.7-fold increase in ppk1 gene transcripts. Ppk1 expression in M. mazei thus appears not to be under classical PHO regulon control.

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“…To prevent cellular damage, all cells have developed various copper detoxification strategies. In prokaryotes, this is mainly achieved by active export of copper ions and in rarer cases by sequestration or exclusion (12,17).…”

Section: Introductionmentioning

confidence: 99%

CopR, a global regulator of transcription to maintain copper homeostasis inPyrococcus furiosus

Grünberger

Reichelt

Waege

et al. 2020

Preprint

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Although copper is in many cases an essential micronutrient for cellular life, higher concentrations are toxic. Therefore, all living cells have developed strategies to maintain copper homeostasis. In this manuscript, we have analysed the transcriptome-wide response of Pyrococcus furiosus to increased copper concentrations and described the essential role of the putative copper-sensing metalloregulator CopR in the detoxification process. To this end, we employed biochemical and biophysical methods to characterise the role of CopR. Additionally, a copR knockout strain revealed an amplified sensitivity in comparison to the parental strain towards increased copper levels, which designates an essential role of CopR for copper homeostasis. To learn more about the CopR-regulated gene network, we performed differential gene expression and ChIP-seq analysis under normal and 20 microM copper-shock conditions. By integrating the transcriptome and genome-wide binding data, we found that CopR binds to the upstream regions of many copper-induced genes. Negative-stain transmission electron microscopy and 2D class averaging revealed an octameric assembly formed from a tetramer of dimers for CopR, similar to published crystal structures from the Lrp family. In conclusion, we propose a model for CopR-regulated transcription and highlight the complex regulatory network that enables Pyrococcus to respond to increased copper concentrations.

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Microbial copper resistance: importance in biohydrometallurgy

Cited by 62 publications

References 105 publications

Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidans ATCC 19703 by Improving Metal Uptake and Tolerance

Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidans ATCC 19703 by Improving Metal Uptake and Tolerance

The potential for polyphosphate metabolism in Archaea and anaerobic polyphosphate formation inMethanosarcina mazei

CopR, a global regulator of transcription to maintain copper homeostasis inPyrococcus furiosus

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