Microbial conversion of solasodine to 1-androstene-3,17-dione (AD), a key intermediate for androgen synthesis

Shukla, Aniruddha; Patil, Sandeep; Bharti, Sudhakar

doi:10.1111/j.1472-765x.1992.tb00732.x

Cited by 8 publications

(6 citation statements)

References 8 publications

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“…A sharp decrease in the bioconversion values was shown by increasing or decreasing the pH from the optimum. Almost similar results were obtained by Sallam et al (1987) and Shukla et al (1992).…”

Section: Optimization Of the Transformation Of Glycosides By A Nidulanssupporting

confidence: 93%

“…On the other hand, gradual decrease in diosgenin bioconversion took place at temperature ranging from 25 to 28°C, and then a sharp decrease was shown by increasing the transformation temperature than 30°C. Nearly the same results were obtained by Sallam et al (1987) and Shukla et al (1992) (Data not shown).…”

Section: Optimization Of the Transformation Of Diosgenin By C Eleganssupporting

confidence: 92%

“…Medium II: Yeast extract, 5; NH 4 NO 3 , 1.0; K 2 HPO 4 , 0.25; MgSO 4 Á7H 2 O, 0.25; FeSO 4 Á7H 2 O, 0.01 (Shukla et al 1992). Medium III: Glucose, 10; peptone, 6; yeast extract, 3; meat extract, 1.5 (Sallam et al 1995).…”

Section: Transformation Mediamentioning

confidence: 99%

“…This increase was followed by a decrease in the product yields of elongation of the incubation period. Shukla et al (1992) stated that, maximum accumulation of AD in the medium was recorded at 192 h Fusarium solani ? ----?…”

Section: Optimization Of the Transformation Of Diosgenin By C Elegansmentioning

confidence: 99%

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Microbial transformation of diosgenin and its precursor furostanol glycosides

Adham

Zaki

Naim

2008

World J Microbiol Biotechnol

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Microorganisms have been screened for biotransformational activity against diosgenin or its precursor furostanol glycosides obtained from fenugreek seed (Trigonella foenum-graecum). Using diosgenin as the substrate, Cunninghamella elegans and Aspergillus nidulans produced some androstenes when a,a 0 -dipyridyl was supplemented to the transformation medium. Also, using the glycosides as a substrate, Rhizopus sp. produced diosgenin in [90% yield from the glycoside. The parameters were optimized to increase the yield of the androstenes.

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Section: Optimization Of the Transformation Of Glycosides By A Nidulanssupporting

confidence: 93%

Section: Optimization Of the Transformation Of Diosgenin By C Eleganssupporting

confidence: 92%

Section: Transformation Mediamentioning

confidence: 99%

Section: Optimization Of the Transformation Of Diosgenin By C Elegansmentioning

confidence: 99%

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Microbial transformation of diosgenin and its precursor furostanol glycosides

Adham

Zaki

Naim

2008

World J Microbiol Biotechnol

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“…Conversion of 16-DPA could be a potential alternative route for production of AD, which is currently dependent mainly on soy sterol bioconversion. Solasodine can be directly converted to AD (Shukla et al, 1992) but lower bioconversion yield is a concern. Moreover, availability of solasodine is limited.…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363

Awadhiya

Banerjee

Patil

2017

Polish Journal of Microbiology

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Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, temperature, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml-1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.

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Steroid Bioconversion

Fernandes¹,

Cabral²

2010

Encyclopedia of Industrial Biotechnology

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Steroids constitute a particular class of lipids characterized by a typical tetracyclic skeleton, composed of one five‐member ring and three fused six‐member rings. Steroids are widely used as therapeutic agents and since their inception in the market, research efforts have been made in order to improve production processes as well as to develop novel synthetic molecules, with enhanced efficiency and reduced side effects. Given the complexity of the steroid skeleton, total chemical synthesis of given steroid molecules is hardly an effective approach; hence, steroid production processes rely on chemical modifications of educts that structurally resemble the targeted product. Still, steroid molecules present multiple chiral centers, a feature that makes particularly appealing the use of the selective microbial catalysts to provide a pathway that does not require protection, and additional deprotection steps. Furthermore, bioconversions are performed in a milder, greener environment, as compared to the purely chemical approach. The present work aims to provide an overview of steroid bioconversions. The potential of biologic agents (microbial cells/enzymes) to catalyze the conversion of a wide array of steroid educts will be presented, with particular emphasis on those that have achieved particular relevance, as well as on key aspects within the field. The chapter ends with the perspective trends in the field of steroid bioconversions

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Microbial conversion of solasodine to 1-androstene-3,17-dione (AD), a key intermediate for androgen synthesis

Cited by 8 publications

References 8 publications

Microbial transformation of diosgenin and its precursor furostanol glycosides

Microbial transformation of diosgenin and its precursor furostanol glycosides

Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363

Steroid Bioconversion

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