Microbial Contamination of Chicken Litter Manure and Antimicrobial Resistance Threat in an Urban Area Setting in Cameroon

Ngogang, Marie Paule; Tambo, Ernest; Kariuki, Jennifer; Mouiche, Mohamed Moctar Mouliom; Ngogang, Jeanne; Wade, Abel; Sande, Marianne A. B. van der

doi:10.3390/antibiotics10010020

Cited by 30 publications

(38 citation statements)

References 23 publications

(17 reference statements)

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“…Resistance to Amoxicillin, Cephalothin (first generation cephalosporin) and erythromycin were also high, above 70% (85%, 81% and 72% respectively) and may be because of selective pressure due to over use of these antibiotics in poultry. These results aligned with the finding from other groups [43,44].…”

Section: Discussionsupporting

confidence: 92%

Multidrug Resistance among Gram Negative E. coli in Chicken Meat (Rafha-Saudi Arabia)

Ali

Chidrawar

2021

JPRI

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Aims: Multidrug resistant (MDR) bacteria pose a major public health issuer globally. The genes for antibiotic resistance are transferred vertically in the form of genomic DNA and horizontally in the form of plasmids or transposons. Antibiotic are extensively used in animal farming to treat and prevent animal diseases, and at sub-therapeutic doses, they are used to promote animal growth. This extensive use of antibiotics is causing an increase in resistance among bacteria. More frequent, chicken meat available at retail shops is reported to be contaminated with a variety of drug resistant bacteria including E. Coli. The aim of the present study was to investigate antibiotic resistance in Escherichia coli strains isolated from chicken meat available in the local shops of Rafha, Saudi Arabia. Place and Duration of Study: Department of basic health sciences, College of Pharmacy, Northern Border University, Rafha, Saudi Arabia, between February and October, 2019 Methodology: Eighty-six E. coli strains, isolated from chicken meat, were tested for their antibiotic resistance profile, using the disc diffusion method. Results: All the isolated E. coli strains were tested against 14 antibiotics. The maximum resistance was found against penicillin G (95%) followed by amoxicillin (85%), Cephalothin (81%), Erythromycin (72%), and Tetracycline (50%). Imipenem was the most effective agent of all with only 1% resistance followed by Cefepime with almost 6% resistance. A high percentage of the isolates (57%,) were multidrug resistant as they were non—susceptible to at least one antimicrobial in ≥3 antimicrobial classes including amoxicillin, erythromycin and tetracycline. Conclusion: The prevalence of MDR E. coli in retail chicken meat is very high and could pose a serious threat to public health.

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Section: Discussionsupporting

confidence: 92%

Multidrug Resistance among Gram Negative E. coli in Chicken Meat (Rafha-Saudi Arabia)

Ali

Chidrawar

2021

JPRI

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“…isolates (Ngogang et al, 2021). The authors reported that all of the tested E. coli isolates showed multi-drug resistant patterns, including one isolate resistant to 9 antibiotics out of 11, and 28% of E. coli isolated cultures were at least resistant to five antibiotics (Ngogang et al, 2021). In parallel 36% of the Salmonella spp.…”

Section: Manure As a Vector Of Antimicrobial Resistancementioning

confidence: 99%

“…E. coli O157 is sensitive to dehydration/desiccation (Jiang et al, 2002;Williams et al, 2005;Vidovic et al, 2007). E. coli persistence was shown at 59.1% of chicken litter samples taken from urban poultry farms in Cameroon (Ngogang et al, 2021). Xing et al (2019) showed that diverse microbial populations reduced the invasive capacity of E. coli (van Elsas et al, 2012).…”

Section: Escherichia Colimentioning

confidence: 99%

The Fate of Foodborne Pathogens in Manure Treated Soil

et al. 2021

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The aim of this review was to provide an update on the complex relationship between manure application, altered pathogen levels and antibiotic resistance. This is necessary to protect health and improve the sustainability of this major farming practice in agricultural systems based on high levels of manure production. It is important to consider soil health in relation to environment and land management practices in the context of the soil microflora and the introduction of pathogens on the health of the soil microbiome. Viable pathogens in manure spread on agricultural land may be distributed by leaching, surface run-off, water source contamination and contaminated crop removal. Thus it is important to understand how multiple pathogens can persist in manures and on soil at farm-scale and how crops produced under these conditions could be a potential transfer route for zoonotic pathogens. The management of pathogen load within livestock manure is a potential mechanism for the reduction and prevention of outbreaks infection with Escherichia coli, Listeria Salmonella, and Campylobacter. The ability of Campylobacter, E. coli, Listeria and Salmonella to combat environmental stress coupled with their survival on food crops and vegetables post-harvest emphasizes the need for further study of these pathogens along with the emerging pathogen Providencia given its link to disease in the immunocompromised and its’ high levels of antibiotic resistance. The management of pathogen load within livestock manure has been widely recognized as a potential mechanism for the reduction and prevention of outbreaks infection but any studies undertaken should be considered as region specific due to the variable nature of the factors influencing pathogen content and survival in manures and soil. Mediocre soils that require nutrients could be one template for research on manure inputs and their influence on soil health and on pathogen survival on grassland and in food crops.

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“…Although the prevalence of colistin resistance is currently relatively low worldwide, colistin-resistant isolates have recently been identified in several Gram-negative bacteria species, such as A. baumannii, K. pneumoniae and P. aeruginosa [10]. Extensive use of colistin in the animal industry has led to increased colistin resistance rates [11]. In 2015, the first plasmid-mediated mobile-colistin resistance (mcr-1) gene in Enterobacteriaceae was discovered in China, and subsequently found in more than 50 countries and regions [7,12,13].…”

Section: Introductionmentioning

confidence: 99%

Determination of Colistin in Contents Derived from Gastrointestinal Tract of Feeding Treated Piglet and Broiler

et al. 2021

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Colistin is considered as the last-resort treatment for multiantibiotic-resistant Gram-negative bacterial infections in humans. However, the oral administration of colistin to livestock and poultry results in the introduction of large amounts of colistin to the surrounding environment via urine and feces, potentially inducing the prevalence of colistin-resistant bacteria and the impact on the ecological environment. We established a quantitative mass spectrometry (MS) based method to measure colistin in contents recovered from the gastrointestinal segments of piglets and broilers, as well as colistin in feces from the animals. The mean recoveries of colistin from different matrices were between 73.2% and 103.9%. The quantitation limit values for different matrices ranged from 0.37 to 1.85 ng/g. In colistin-treated swine samples, the highest concentration of colistin was detected in feces samples at a level of 1248.3 ng/g. However, the highest concentration of colistin in broiler samples was around 4882.9 ng/g, which was found in the contents derived from broilers’ ceca. The employment of the proposed method to assess colistin in animals’ gastrointestinal tracts might help to understand the colistin absorption in animals’ guts and the potential impact of colistin on the emergence of resistant bacteria in animals’ gut flora and the ecological environment.

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Microbial Contamination of Chicken Litter Manure and Antimicrobial Resistance Threat in an Urban Area Setting in Cameroon

Cited by 30 publications

References 23 publications

Multidrug Resistance among Gram Negative E. coli in Chicken Meat (Rafha-Saudi Arabia)

Multidrug Resistance among Gram Negative E. coli in Chicken Meat (Rafha-Saudi Arabia)

The Fate of Foodborne Pathogens in Manure Treated Soil

Determination of Colistin in Contents Derived from Gastrointestinal Tract of Feeding Treated Piglet and Broiler

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