Determination of Colistin in Contents Derived from Gastrointestinal Tract of Feeding Treated Piglet and Broiler

Abstract

Colistin is considered as the last-resort treatment for multiantibiotic-resistant Gram-negative bacterial infections in humans. However, the oral administration of colistin to livestock and poultry results in the introduction of large amounts of colistin to the surrounding environment via urine and feces, potentially inducing the prevalence of colistin-resistant bacteria and the impact on the ecological environment. We established a quantitative mass spectrometry (MS) based method to measure colistin in contents recovered from the gastrointestinal segments of piglets and broilers, as well as colistin in feces from the animals. The mean recoveries of colistin from different matrices were between 73.2% and 103.9%. The quantitation limit values for different matrices ranged from 0.37 to 1.85 ng/g. In colistin-treated swine samples, the highest concentration of colistin was detected in feces samples at a level of 1248.3 ng/g. However, the highest concentration of colistin in broiler samples was around 4882.9 ng/g, which was found in the contents derived from broilers’ ceca. The employment of the proposed method to assess colistin in animals’ gastrointestinal tracts might help to understand the colistin absorption in animals’ guts and the potential impact of colistin on the emergence of resistant bacteria in animals’ gut flora and the ecological environment.

Global epidemiology, genetic environment, risk factors and therapeutic prospects of mcr genes: A current and emerging update

“…The pharmaceutical form of colistin is indicated for oral administration and is present in a powder or solution form (Hille et al, 2018). Studies have shown that the drug is poorly absorbed in the digestive tract after oral administration and can be excreted in high levels through the feces (Rhouma et al, 2015;Peng et al, 2021). This results in the presence of colistin or its active metabolites in the environment alongside the MCRPE isolates from the animal feces (Shen et al, 2018).…”

Disruption of the primocolonizing microbiota alters epithelial homeostasis and imprints stem cells in the colon of neonatal piglets

“…LPS from Escherichia coli O111:B4 (Sigma‐Aldrich, L2630) was used at 0.1, 1, and 10 μg/mL based on previous studies in intestinal organoids 36–38 . Colistin sulfate (Biove, Vetocoli) was used at 1, 10, and 100 μg/mL based on quantification of colistin in the gastrointestinal tract of piglets treated with this antibiotic 26,39,40 . The treatments were refreshed after 24 h. Transepithelial electrical resistance (TEER) was measured with an Epithelial Volt Ohm Meter (EVOM3, World Precision Instruments) according to the manufacturer's instructions.…”

Challenges of Colistin Use in ICU and Therapeutic Drug Monitoring: A Literature Review

“…The most frequently mentioned product ion corresponds to values in the range m / z 100.9–101.4 [ 26 , 27 ]. The product ions and a summary of the selected methods are listed in Table 1 [ 17 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ].…”