Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing

Ma, Xinbin; Wu, Chongde; Huang, Jun; Zhou, Rongqing; Bi, Shuping

doi:10.4014/jmb.1712.12054

Cited by 17 publications

(6 citation statements)

References 44 publications

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“…In the review of Ferrera and Sanchez [21] some results of molecular studies on Archaea detection in various types of wastewater treatment system are presented. As described in literature, Archaea have been detected by the 454 pyrosequencing and Illumina, in the following samples from WWTPs: activated sludges, anaerobic sludges, effluent from swine WWTPs, anaerobic digester sludge, sludge from anaerobic reactors [10,11,29,[34][35][36][37][38][39]. Our results are the first to describe the changes of archaeal communities under the technological process in an operating full-scale municipal wastewater treatment plant by the Illumnia HiSeq platform (metagenomic shotgun sequencing).…”

Section: Plos Onesupporting

confidence: 56%

“…Crenarchaeota was the second most abundant phylum ranging from 3.5% in the raw wastewater (sample S1) to 9.5% in the sewage sludge from the outlet of the mechanical concentrator (sample S9) with average 6.6%. Euryarchaeota was also detected by Illumina MiSeq sequencing approach as the predominant phyla in tannery WWTP [29] and in other previous studies on activated sludge WWTPs [30,31]. Ma et al [29] compared the diversity and richness of the tannery wastewater with the municipal wastewater, and concluded that the two types of wastewater were similar in composition and structure.…”

Section: Plos Onementioning

confidence: 90%

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Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea

et al. 2021

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Due to limited description of the role and diversity of archaea in WWTPs, the aim of the study was to analyze microbial community structures and diversities with particular regard to Archaea in the samples taken from different stages of the full-scale municipal wastewater treatment plant and effluent receiving water (upstream and downstream discharge point). Our study was focused on showing how the treatment processes influenced the Eubacteria and Archaea composition. Alpha and Beta diversity were used to evaluate the microbial diversity changes in the collected samples. Proteobacteria was the largest fraction ranging from 28% to 67% with 56% relative abundance across all samples. Archaea were present in all stages of WWTP ranged from 1 to 8%. Among the Archaea, two groups of methanogens, acetoclastic (Methanosarcina, Methanosaeta) and hydrogenotrophic methanogens (Methanospirillium, Methanoculleus, Methanobrevibacter) were dominant in the technological stages. The obtained results indicate that the treated wastewater did not significantly affect eubacterial and archaeal composition in receiving water. However, differences in richness, diversity and microbial composition of Eubacteria and Archaea between the wastewater samples taken from the primary and secondary treatment were observed.

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Section: Plos Onesupporting

confidence: 56%

Section: Plos Onementioning

confidence: 90%

Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea

et al. 2021

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“…Our analysis suggests that Proteobacteria may play a crucial role in the detoxification and assimilation of both organic and inorganic substances, including heavy metal contaminants, in sediment samples. Earlier many researchers have reported the dominant phylum as Proteobacteria in several waste-contaminated sites, such as solid waste dumping site ( Kumar R. et al, 2021 ), distillery waste ( Kumar and Chandra, 2020 ), antibiotic containing waste ( Marathe et al, 2016 ), tannery wastewater ( Ma et al, 2018 ), electronic waste polluted site ( Salam and Varma, 2019 ) and their important role in pollutant removal. The heavy metals/metalloids and pH significantly affected the abundance and structure of most microorganisms ( Zhao et al, 2020 ; Hu et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Unraveling the shift in bacterial communities profile grown in sediments co-contaminated with chlorolignin waste of pulp-paper mill by metagenomics approach

Kumar,

Ameen,

Verma

2024

Front. Microbiol.

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Pulp-paper mills (PPMs) are known for consistently generating a wide variety of pollutants, that are often unidentified and highly resistant to environmental degradation. The current study aims to investigate the changes in the indigenous bacterial communities profile grown in the sediment co-contaminated with organic and inorganic pollutants discharged from the PPMs. The two sediment samples, designated PPS-1 and PPS-2, were collected from two different sites. Physico-chemical characterization of PPS-1 and PPS-2 revealed the presence of heavy metals (mg kg−1) like Cu (0.009–0.01), Ni (0.005–0.002), Mn (0.078–0.056), Cr (0.015–0.009), Pb (0.008–0.006), Zn (0.225–0.086), Fe (2.124–0.764), Al (3.477–22.277), and Ti (99.792–45.012) along with high content of chlorophenol, and lignin. The comparative analysis of organic pollutants in sediment samples using gas chromatography–mass spectrometry (GC–MS) revealed the presence of major highly refractory compounds, such as stigmasterol, β-sitosterol, hexadecanoic acid, octadecanoic acid; 2,4-di-tert-butylphenol; heptacosane; dimethyl phthalate; hexachlorobenzene; 1-decanol,2-hexyl; furane 2,5-dimethyl, etc in sediment samples which are reported as a potential toxic compounds. Simultaneously, high-throughput sequencing targeting the V3–V4 hypervariable region of the 16S rRNA genes, resulted in the identification of 1,249 and 1,345 operational taxonomic units (OTUs) derived from a total of 115,665 and 119,386 sequences read, in PPS-1 and PPS-2, respectively. Analysis of rarefaction curves indicated a diversity in OTU abundance between PPS-1 (1,249 OTUs) and PPS-2 (1,345 OTUs). Furthermore, taxonomic assignment of metagenomics sequence data showed that Proteobacteria (55.40%; 56.30%), Bacteoidetes (11.30%; 12.20%), and Planctomycetes (5.40%; 4.70%) were the most abundant phyla; Alphproteobacteria (20.50%; 23.50%), Betaproteobacteria (16.00%; 12.30%), and Gammaproteobacteria were the most recorded classes in PPS-1 and PPS-2, respectively. At the genus level, Thiobacillus (7.60%; 4.50%) was the most abundant genera grown in sediment samples. The results indicate significant differences in both the diversity and relative abundance of taxa in the bacterial communities associated with PPS-2 when compared to PPS-1. This study unveils key insights into contaminant characteristics and shifts in bacterial communities within contaminated environments. It highlights the potential for developing efficient bioremediation techniques to restore ecological balance in pulp-paper mill waste-polluted areas, stressing the importance of identifying a significant percentage of unclassified genera and species to explore novel genes.

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“…The presence of the same OTU in four different treatment pools indicates that the same bacteria exist at different stages of treatment. Ma et al investigated the microbial communities in three tannery wastewater treatment plants using Illumina MiSeq sequencing; the same microorganisms were found in all three plants, proving that the wastewater source did not directly cause the microbial community structure in the wastewater treatment system [27]. The microbial community structures formed in different processing units differ because of variations in the environmental conditions surrounding them.…”

Section: Diversity Analysis For Bacterial Communitiesmentioning

confidence: 99%

“…community structure in the wastewater treatment system [27]. The microbial community structures formed in different processing units differ because of variations in the environmental conditions surrounding them.…”

Section: Diversity Analysis For Bacterial Communitiesmentioning

confidence: 99%

Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment System

Shi

Liu

et al. 2021

Microorganisms

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Chemicals of emerging concern (CEC) in pig farm breeding wastewater, such as antibiotics, will soon pose a serious threat to public health. It is therefore essential to consider improving the treatment efficiency of piggery wastewater in terms of microorganisms. In order to optimize the overall piggery wastewater treatment system from the perspective of the bacterial community structure and its response to environmental factors, five samples were randomly taken from each area of a piggery’s wastewater treatment system using a random sampling method. The bacterial communities’ composition and their correlation with wastewater quality were then analyzed using Illumina MiSeq high-throughput sequencing. The results showed that the bacterial community composition of each treatment unit was similar. However, differences in abundance were significant, and the bacterial community structure gradually changed with the process. Proteobacteria showed more adaptability to an anaerobic environment than Firmicutes, and the abundance of Tissierella in anaerobic zones was low. The abundance of Clostridial (39.02%) and Bacteroides (20.6%) in the inlet was significantly higher than it was in the aerobic zone and the anoxic zone (p < 0.05). Rhodocyclaceae is a key functional microbial group in a wastewater treatment system, and it is a dominant microbial group in activated sludge. Redundancy analysis (RDA) showed that chemical oxygen demand (COD) had the greatest impact on bacterial community structure. Total phosphorus (TP), total nitrogen (TN), PH and COD contents were significantly negatively correlated with Sphingobacteriia, Betaproteobacteria and Gammaproteobacteria, and significantly positively correlated with Bacteroidia and Clostridia. These results offer basic data and theoretical support for optimizing livestock wastewater treatment systems using bacterial community structures.

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Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing

Cited by 17 publications

References 44 publications

Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea

Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea

Unraveling the shift in bacterial communities profile grown in sediments co-contaminated with chlorolignin waste of pulp-paper mill by metagenomics approach

Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment System

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