Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea

Płaza, Grażyna; Jałowiecki, Łukasz; Głowacka, Dominika; Hubeny, Jakub; Harnisz, Monika; Korzeniewska, Ewa

doi:10.1371/journal.pone.0250514

Cited by 13 publications

(6 citation statements)

References 50 publications

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“…1b). The higher diversity of microbial communities in the outlet of the wastewater treatment plant indicates that the treatment process effectively removes pollutants and facilitates the growth of diverse microbial communities [33,34]. Previous studies have reported similar findings, supporting the idea that wastewater treatment processes can enhance microbial diversity in effluent.…”

Section: Resultssupporting

confidence: 61%

“…Previous studies have reported similar findings, supporting the idea that wastewater treatment processes can enhance microbial diversity in effluent. For instance, a study by Płaza et al [33] investigated the microbial communities in WWTP and found that the treated effluent displayed different microbial diversity compared to the influent. The higher microbial diversity observed in the outlet of the wastewater treatment plant has significant implications for ecosystem health.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

PacBio Sequencing Reveals Microbial Community Diversity in Man-made and Natural Habitats

Gad,

Yosri,

Alqhtani

et al. 2023

Pol. J. Environ. Stud.

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Prokaryotic communities play a pivotal role in maintaining ecosystem balance through their involvement in essential processes including carbon fixation, nutrient cycling, and the decomposition of organic matter. Despite the importance of prokaryotic communities in wastewater treatment plants and rivers, their diversity and distribution in these environments in Egypt are not well understood. To bridge this gap, the study utilized next-generation 16S rRNA amplicon sequencing based on PacBio technology to investigate the composition and diversity of these microbial communities in wastewater treatment plant (inlet and outlet) and the Nile River. The principal coordinate analysis showed that the microbial community structure varied significantly between the three habitats, indicating the wastewater treatment process effectively removes pollutants and facilitates the growth of diverse microbial communities. Proteobacteria increased in outlet and surface water (>50%) versus inlet (45%), while Actinobacteria increased in surface water (>20%). Firmicutes and Campilobacteria decreased significantly (P≤0.05) in outlet versus inlet, and Chloroflexi were only found in outlet (<2%). Environmental factors such as EC and NH 4 -N were the most significant variables in explaining the variation in microbial communities. It's worth noting that the final effluent from the Zenin WWTP aligns with the standards set by the Egyptian ministerial decree 48/1982 for discharging in surface water bodies. Understanding the composition

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Section: Resultssupporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%

PacBio Sequencing Reveals Microbial Community Diversity in Man-made and Natural Habitats

Gad,

Yosri,

Alqhtani

et al. 2023

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

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“…During the sampling campaigns, 30 grab samples were collected in the sampling points presented in Figure 1 . The sampling and transportation procedures used are described by Rolbiecki et al [ 22 ] and Płaza et al [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

“…Sequencing results were uploaded to the MetaGenome Rapid Annotation Subsystems Technology (MG-RAST version 4.0.3) server as FASTQ files for analysis [ 24 ]. Details of the metagenomic analysis are presented in the paper by Płaza et al [ 23 ]. The sequences were submitted to NCBI database, and they are under BioProject number ID: PRJNA666519.…”

Section: Methodsmentioning

confidence: 99%

Seasonal and Technological Shifts of the WHO Priority Multi-Resistant Pathogens in Municipal Wastewater Treatment Plant and Its Receiving Surface Water: A Case Study

Jałowiecki

Hubeny

Harnisz

et al. 2021

IJERPH

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The present study was focused on the identification of multi-resistant bacteria from the WHO priority pathogens list in the samples taken from different stages of the full-scale municipal wastewater treatment plant and receiving water. Additionally, the seasonal variations of the selected multi-resistant pathogens were analyzed in the samples. In order to the aim of the study, the metagenomic DNA from the collected samples was isolated and sequenced. The samples were collected in three campaigns (spring, summer, autumn). Metagenomic DNA was isolated by the commercial kits, according to the manufacturer’s instruction. Illumina sequencing system was employed, and the R program was used to metagenomic analysis. It was found that the wastewater samples and receiving water contained the multi-resistant bacteria from the WHO priority pathogens list. The seasonal and technological variations affected the distribution of the pathogens in the wastewater. No effect of the effluent on the pathogens in the receiving water was observed. The results indicated that antibiotic-resistant “priority pathogens” from the WHO list are there in the waste- and receiving water. Technological process and seasons effected their distribution in the environment. Metagenomic analysis can be used as sufficient tool in microbiological and human health risk assessment.

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“…This indicates that pretreatments strengthened its position in the dominant genus. Methanoculleus can use ethanol and some secondary alcohols as electron donors, as they produce methane [37]. This is also one of the important reasons for increasing methane production.…”

Section: Methanogenic Archaeal Community For Different Pretreatmentsmentioning

confidence: 99%

Effects of Iron Powder Addition and Thermal Hydrolysis on Methane Production and the Archaeal Community during the Anaerobic Digestion of Sludge

Cao

Wang

Liu

2022

IJERPH

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The conventional anaerobic digestion of sludge has the disadvantages of long digestion time and low methane production. Pretreatment is often used to mitigate these problems. In this study, three pretreatment methods, namely, the addition of iron powder, high-temperature thermal hydrolysis, and a combination of these methods, were compared for application with conventional continuous anaerobic digestion reactors. The results showed that pretreatment improved methane yield by 18.2–22.9%, compared to the control reactor (conventional anaerobic digestion). Moreover, it was recognized that the archaeal community in the sludge underwent significant changes after pretreatment. Specifically, the addition of iron powder reduced the diversity in the archaeal community, but increased the abundance of hydrogenotrophic methanogens without changing the community composition. Thermal hydrolysis at high temperatures had the reverse effect, as it increased the diversity of the archaeal community but inhibited the growth of acetoclastic methanogens. In the case of the combined pretreatment, the thermal hydrolysis had a dominant influence on the archaeal community. By comparing the changes in functional gene content, it was found that the functional abundance of the archaeal community in the transport and metabolism of carbohydrates, lipids, and amino acids was higher after pretreatment than in the control group.

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Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea

Cited by 13 publications

References 50 publications

PacBio Sequencing Reveals Microbial Community Diversity in Man-made and Natural Habitats

PacBio Sequencing Reveals Microbial Community Diversity in Man-made and Natural Habitats

Seasonal and Technological Shifts of the WHO Priority Multi-Resistant Pathogens in Municipal Wastewater Treatment Plant and Its Receiving Surface Water: A Case Study

Effects of Iron Powder Addition and Thermal Hydrolysis on Methane Production and the Archaeal Community during the Anaerobic Digestion of Sludge

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