Microbial Community in the Forestomachs of Alpacas (Lama pacos) and Sheep (Ovis aries)

Animal Feed Science and Technology

et al. 2016

Section: Extraction Of Dna and Quantitative Real-time Pcrmentioning

confidence: 99%

Section: Extraction Of Dna and Quantitative Real-time Pcrmentioning

confidence: 99%

Effects of rumen-protected folic acid on ruminal fermentation, microbial enzyme activity, cellulolytic bacteria and urinary excretion of purine derivatives in growing beef steers

Animal Feed Science and Technology

et al. 2016

“…Microbial DNA was extracted from 1.0 ml ruminal fluid with repeated bead beating plus column (Pei et al., ). The extracted DNA was kept frozen at −20°C for PCR.…”

Section: Methodsmentioning

confidence: 99%

Effects of concentrate‐to‐forage ratios and 2‐methylbutyrate supplementation on ruminal fermentation, bacteria abundance and urinary excretion of purine derivatives in Chinese Simmental steers

Animal Physiology Nutrition

et al. 2018

This study evaluated the effects of dietary concentrate levels and 2-methylbutyrate (2MB) supplementation on performance, ruminal fermentation, bacteria abundance, microbial enzyme activity and urinary excretion of purine derivatives (PD) in steers. Eight ruminally cannulated Simmental steers (12 months of age; 389 ± 3.7 kg of body weight) were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement. Moderate-concentrate (400 g/kg diet [MC]) or high-concentrate (600 g/kg diet [HC]) diets were fed with or without 2MB (0 g/day [2MB-] or 15.0 g/day [2MB+]). Dry matter intake and average daily gain increased, but feed conversion ratio decreased with the HC diet or 2MB supplementation. Ruminal pH decreased, but total volatile fatty acid increased with the HC diet or 2MB supplementation. Molar proportion of acetate and acetate-to-propionate ratio decreased with the HC diet, but increased with 2MB supplementation. Propionate molar proportion and ruminal NH -N content increased with the HC diet, but decreased with 2MB supplementation. Neutral detergent fibre degradability decreased with the HC diet, but increased with 2MB supplementation. Crude protein degradability increased with the HC diet or 2MB supplementation. Abundance of Ruminococcus albus, Ruminococcus flavefaciens, Fibrobacter succinogenes and Bufyrivibrio fibrisolvens as well as activities of carboxymethyl cellulase, cellobiase, xylanase and pectinase decreased with the HC diet, but increased with 2MB supplementation. However, abundance of Prevotella ruminicola and Ruminobacter amylophilus as well as activities of α-amylase and protease increased with the HC diet or 2MB supplementation. Total PD excretion also increased with the HC diet or 2MB supplementation. The results suggested that growth performance, ruminal fermentation, CP degradability and total PD excretion increased with increasing dietary concentrate level from 40% to 60% or 2MB supplementation. The observed diet × 2MB interaction indicated that supplementation of 2MB was more efficacious for improving growth performance, ruminal fermentation and total PD excretion with promoted ruminal bacteria abundance and enzyme activity in the MC diet than in the HC diet.

show abstract

“…According to the method of Pei et al. (), microbial DNA was extracted from 1.0 ml ruminal fluid with repeated bead beating plus column. The extracted DNA was kept frozen at −20°C for PCR.…”

Section: Methodsmentioning

confidence: 99%

“…The extracted DNA was kept frozen at −20°C for PCR. Populations of F. succinogenes, R. albus, R. flavefaciens, B. fibrisolvens, P. ruminicola and R. amylophilus were performed with real‐time PCR as a proportion of total bacterial 16S rDNA according to the formula (Pei et al., ):

Relative quantification = 2^{- (normalCycle normalthreshold false[normalCt false] normaltarget - normalCt normaltotal normalbacteria)}

…”

Section: Methodsmentioning

confidence: 99%

Effects of dietary protein levels and 2‐methylbutyrate on ruminal fermentation, nutrient degradability, bacterial populations and urinary purine derivatives in Simmental steers

Animal Physiology Nutrition

et al. 2017