Microbial Community Diversity of a Water Body in the Niger Delta as Affected by Refinery Effluent

Obiukwu, CE; Otokunefor, T. V.

doi:10.13189/ujmr.2014.020101

Cited by 5 publications

(1 citation statement)

References 44 publications

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“…Ogbonna et al [35] reported higher total bacteria counts (1.12±0.13x10 8 to 1.28±0.09x10 8 cfu/m) in surface water. The bacteria and fungi isolated and identified in this study are similar to those reported by several researchers in similar ecosystems [26,36,37]. The preponderance of coliform group of bacteria in the estuary water in our study is a calls for concern as it indicates recent faecal contamination [29,38].…”

Section: Discussionsupporting

confidence: 88%

Physicochemical and Microbiological Assessment of Utaewa Estuary Water

Ekanem,

Itah,

Ndubuisi-Nnaji

et al. 2023

JAMB

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Most communities in the oil rich Niger Delta find it difficult to access to potable water due to constant pollution of their natural water sources. The sole aim of this study was to examine the microbiological and physicochemical qualities of the Estuary water at Utaewa to ascertain its suitability for potable use. Standard culture dependent techniques as well as metagenomics approach using Next Generation Sequencing (NGS) on Illumina Miseq platform was used to determine the microbiological characteristics while standard analytical procedures were employed to ascertain the physicochemical properties of Utaewa in Imo River. The average microbial counts ranged as follows: Total Heterotrophic Bacteria; 2.17±0.08x106 to 2.23±0.08x106 cfu/ml, Hydrocarbon Utilizing Bacteria; 1.03±0.06x105 to 1.39±0.08x105 cfu/ml, Total Heterotrophic Fungi; 1.23±0.13x105 to 1.64±0.09x105 cfu/ml, Hydrocarbon Utilizing Fungi; 7.9±0.21x103 to 8.6±1.00x103 cfu/ml. Bacterial isolates belonging to the genera Escherichia, Citrobacter, Bacillus, Salmonella, Shigella, Proteus, Flavobacterium, Vibrio, Micrococcus and Pseudomonas. were isolated and identified. The fungal isolates belonged to the genera Aspergillus, Rhizopus, Fusarium, Penicillium, Saccharomyces and Candida. On the other hand, Unknown (50.16%), Acinetobacter (8.34%), Ignatzschineria (4.73%), Planctomyces (3.28%), Anaerospora (3.27%), RS62 (2.73%), Methylophaga (1.64%), Wohlfahrtiimonas (1.31%), Myroides (1.23%) and Candidatus (0.95%) were captured by metagenomics analysis at the generic level. All the physicochemical parameters conformed to WHO and NIS standards for potable water except for Turbidity and Magnesium. Cultural methods used in this study were able to identify many potential water borne pathogens, metagenomics captured more microbial groups and give a better insight to bacterial composition and diversity. The presence of theses pathogenic bacteria underlies poor water quality and can pose public health threat to man and aquatic fauna. There is need to adhere to good hygienic practices and minimize the direct discharge of waste without proper treatment.

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Section: Discussionsupporting

confidence: 88%

Physicochemical and Microbiological Assessment of Utaewa Estuary Water

Ekanem,

Itah,

Ndubuisi-Nnaji

et al. 2023

JAMB

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Factors influencing the alteration of microbial and heavy metal characteristics of river systems in the Niger Delta region of Nigeria

Izah

Aigberua²,

Srivastav

2022

Ecological Significance of River Ecosystems

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Screening of polycyclic aromatic hydrocarbon degrading bacterial isolates from oil refinery wastewater and detection of conjugative plasmids in polycyclic aromatic hydrocarbon tolerant and multi-metal resistant bacteria

Khatoon

Malik

2019

Heliyon

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Wastewater were collected from the effluent channel in the vicinity of Mathura oil refinery, U.P. (India) and analysed for physicochemical characteristics, heavy metals as well as organic compounds including PAHs. The interaction of PAHs and heavy metals with various group of microorganisms revealed the viable count of aerobic heterotrophs, asymbiotic nitrogen fixers, actinomycetes and fungi were found to be 2.38 Â 10 6 , 1.89 Â 10 4 , 2.20 Â 10 4 CFU/mL and 8.76 Â 10 3 CFU/mL respectively. We have selected and screened 50 bacterial isolates for their resistance/tolerance to heavy metal and PAHs. Out of 25 multi-metal resistant isolates, 6 were able to tolerate PAHs at the concentration of 5000 μg/mL (50μg/disc) to naphthalene, anthracene, phenanthrene and pyrene. The PAH degradation efficiency of the isolates was assessed using spectrophotometer with 100 μg/mL of phenanthrene and observed different degree of degradation ranging from 34-66% after 96 h of incubation. One of the bacterial isolates KWB3 (identified as Enterobacter ludwigii by 16S rDNA sequencing) exhibited maximum degradation efficiency (66%) was further tested for phenanthrene degrading ability in the presence and absence of a co-substrate (glucose) in a mineral salt medium; and a number of metabolites were produced and detected by GC-MS which revealed the presence of benzocoumarin, phthalic acid, catechol and several low molecular weight compounds. The DNA derived from multi-metal and PAHs tolerant bacteria were PCR amplified using Inc specific primers and positive PCR products were obtained with oriT and trfA2 of the IncP group; indicates that these bacteria have gene-mobilizing capacity.

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Microbial Community Diversity of a Water Body in the Niger Delta as Affected by Refinery Effluent

Cited by 5 publications

References 44 publications

Physicochemical and Microbiological Assessment of Utaewa Estuary Water

Physicochemical and Microbiological Assessment of Utaewa Estuary Water

Factors influencing the alteration of microbial and heavy metal characteristics of river systems in the Niger Delta region of Nigeria

Screening of polycyclic aromatic hydrocarbon degrading bacterial isolates from oil refinery wastewater and detection of conjugative plasmids in polycyclic aromatic hydrocarbon tolerant and multi-metal resistant bacteria

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