Microbial community analysis of anaerobic granules in phenol-degrading UASB by next generation sequencing

Na, Jeong-Geol; Lee, Chang Kyu; Yun, Yeo-Myeong; Moon, Christina H.; Kim, Mi-Sun; Kim, Dong-Hoon

doi:10.1016/j.bej.2016.04.030

Cited by 72 publications

(22 citation statements)

References 28 publications

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“…saturated four to eight fatty acids (Jackson et al 1999, McInerney et al 1981, Wallrabenstein and Schink 1994. Both Syntrophus aciditrophicus and Syntrophus buswellii could also degrade benzoate, which is the intermediate during phenol degradation (Na et al 2016).…”

Section: Microbial Community Compositions As Revealed By High-throughmentioning

confidence: 99%

Biogas production from hydrothermal liquefaction wastewater (HTLWW): Focusing on the microbial communities as revealed by high-throughput sequencing of full-length 16S rRNA genes

et al. 2016

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Hydrothermal liquefaction (HTL) is an emerging and promising technology for the conversion of wet biomass into bio-crude, however, little attention has been paid to the utilization of hydrothermal liquefaction wastewater (HTLWW) with high concentration of organics. The present study investigated biogas production from wastewater obtained from HTL of straw for bio-crude production, with focuses on the analysis of the microbial communities and characterization of the organics. Batch experiments showed the methane yield of HTLWW (R-HTLWW) was 184 mL/g COD, while HTLWW after petroleum ether extraction (PE-HTLWW), to extract additional bio-crude, had higher methane yield (235 mL/g COD) due to the extraction of recalcitrant organic compounds. Sequential batch experiments further demonstrated the higher methane yield of PE-HTLWW. LC-TOF-MS, HPLC and gel filtration chromatography showed organics with molecular weight (MW) < 1000 were well degraded. Results from the high-throughput sequencing of full-length 16S rRNA genes analysis showed similar microbial community compositions were obtained for the reactors fed with either R-HTLWW or PE-HTLWW. The degradation of fatty acids were related with Mesotoga infera, Syntrophomonas wolfei et al. by species level identification. However, the species related to the degradation of other compounds (e.g. phenols) were not found, which could be due to the presence of uncharacterized microorganisms. It was also found previously proposed criteria (97% and 98.65% similarity) for species identification of 16S rRNA genes were not suitable for a fraction of 16S rRNA genes.

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Section: Microbial Community Compositions As Revealed By High-throughmentioning

confidence: 99%

Biogas production from hydrothermal liquefaction wastewater (HTLWW): Focusing on the microbial communities as revealed by high-throughput sequencing of full-length 16S rRNA genes

et al. 2016

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“…Recently, anaerobic biotechnology has been widely used in the treatment of phenolic wastewater due to its characteristics of low sludge yield, good tolerance and decomposing ability to phenols [6][7][8]. The strict environmental requirement is one of the main limiting factors for its industrial application.…”

Section: Introductionmentioning

confidence: 99%

Potential impact of methyl isobutyl ketone (MIBK) on phenols degradation in an UASB reactor and its degradation properties

Wang

Yang

Sierra

et al. 2017

Journal of Hazardous Materials

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Methyl isobutyl ketone (MIBK) as a solvent is extensively used for the phenols extraction from the wastewater, so it is unavoidable to expose in the effluent due to the solubility and leakage problem. The present study evaluated the impact of MIBK on phenols degradation in an UASB reactor and analyzed its degradation properties. The results indicated that the continuous dosing (0.1gL) and impact (10gL) of MIBK had limited effect on phenols removal (1-2% reduction) in the UASB reactor, but the specific methanogenic activity (SMA) values of sludge decreased by 45-75% after MIBK exposure. Anaerobic degradation rate of MIBK fitted well to a pseudo-first-order kinetic equation with respect to the initial concentration of 35mgL (k=0.0115h, R=0.9664). Furthermore, the relative methane generation rate constants of MIBK were 0.00816, 0.00613, 0.00273, and 0.00207d at the initial concentrations of 0.1, 0.5, 5, and 10gL, respectively. MIBK showed higher inhibitory effect on the methanogenesis than on phenols degradation. This study pointed out that the industrial installations should consider the influence of solvent on anaerobic treatment of phenolic wastewater.

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“…In a previous study, 53 Methanosaeta and Methanobacterium are reported as dominant genera involved in phenol degradation after phenol conversion to benzoate (phenol → benzoate → acetate + H 2 ) by some Proteobacteria (Syntrophus) by the following conversion pathways: acetate → CH 4 , and hydrogen → CH 4 , respectively. However, in the liquid phase acetoclastic methanogens were more abundant (54.8%) than hydrogenotrophic ones (33.2%) as a result of the increase in the Methanosaeta percentage at the expense of the Methanobacterium as compared to the biofilm.…”

mentioning

confidence: 99%

“…This shift between hydrogenotrophic methanogens and acetotrophic methanogens can be also linked to the presence of high phenols concentration. In a previous study, 53 Methanosaeta and Methanobacterium are reported as dominant genera involved in phenol degradation after phenol conversion to benzoate (phenol → benzoate → acetate + H 2 ) by some Proteobacteria (Syntrophus) by the following conversion pathways: acetate → CH 4 , and hydrogen → CH 4 , respectively.…”

mentioning

confidence: 99%

Performance and microbial analysis during long‐term anaerobic digestion of olive mill wastewater in a packed‐bed biofilm reactor

Enaime

Nettmann

Berzio

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: The treatment of olive mill wastewater (OMWW) in a packed bed biofilm reactor (PBBR) and the identification of different microorganisms involved in the digestion process is an attractive field for research. RESULTS: A PBBR treating OMWW under different organic loading rates (OLRs) (0.94-9.36 gCOD/(L d)), showed high stability during an extensive time (723 days) without any sign of acidification. High volumetric biogas and methane (CH 4 ) productions, 4.1 and 2.4 L N /(L reactor d), respectively were registered at the highest OLR. Meanwhile, high chemical oxygen demand (COD) removal (62.5-79.7%) was obtained throughout the experiment. Phenolic compounds removal was much higher during the first steps of the experiment (75.9-84.2%), and decreased as the OLR increased to about 38%. High homogeneity of biofilm thickness and Bacteria and extracellular polymeric substance distribution were demonstrated by confocal laser scanning microscopy. Results of real-time quantitative polymerase chain reaction (PCR) and 16S rRNA amplicon analyses showed that Bacteria, mainly consisting of Firmicutes, Proteobacteria, Bacteroidetes andChloroflexi phylum, was about eight times more abundant than Archaea in the reactor liquid phase, while Bacteria to Archaea ratio was almost one in the biofilm. This points to a major degradation of organic substances in the liquid, while methanogenesis occurred mainly within the biofilm. In the liquid, acetoclastic methanogens were more abundant than hydrogenotrophic ones, while both groups showed likely very similar contributions in the biofilm.CONCLUSION: Promising results have been presented throughout this work providing information about the long-term operation of PBBR-granular activated carbon treating OMWW and analyzing, in an efficient manner, the structure and the composition of the microbial community involved in OMWW digestion.

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Microbial community analysis of anaerobic granules in phenol-degrading UASB by next generation sequencing

Cited by 72 publications

References 28 publications

Biogas production from hydrothermal liquefaction wastewater (HTLWW): Focusing on the microbial communities as revealed by high-throughput sequencing of full-length 16S rRNA genes

Biogas production from hydrothermal liquefaction wastewater (HTLWW): Focusing on the microbial communities as revealed by high-throughput sequencing of full-length 16S rRNA genes

Potential impact of methyl isobutyl ketone (MIBK) on phenols degradation in an UASB reactor and its degradation properties

Performance and microbial analysis during long‐term anaerobic digestion of olive mill wastewater in a packed‐bed biofilm reactor

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