Microbial Biofilms

Singh, Niraj Kumar; Das, Pranjal Pratim

doi:10.1002/9781119830795.ch15

Cited by 2 publications

(1 citation statement)

References 119 publications

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“…Interestingly, our results with the LF‐89 type strain indicate that the treatment with an over‐MIC (1 μg/mL) dose of FLO also decreased the bacterial count in both materials used; however, the antibiotic was more effective against the planktonic bacteria generating a higher reduction of live P. salmonis than when the bacteria is part of the biofilm. In addition, several reports indicates that the biofilm‐embedded bacteria are less susceptible to antimicrobials and/or could develop antibiotic resistance in its natural environment, which could be acquired from other microorganisms via transference of genetic material by plasmids and extracellular vesicles, among others (Dincer et al, 2020; Hall & Mah, 2017; Singh & Das, 2022). However, our study evaluated only P. salmonis in an in vitro system, so the decrease in the effect of the FLO on the count of live bacteria seems to be generated by the biofilm matrix (Marshall et al, 2012), which confers a protected environment for the bacteria.…”

Section: Discussionmentioning

confidence: 99%

Effect of florfenicol on Piscirickettsia salmonis biofilm formed in materials used in salmonid nets, nylon and high‐density polyethylene

Oliver,

Ruiz,

Vidal

et al. 2023

Journal of Fish Diseases

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Piscirickettsiosis is the most prevalent bacterial disease affecting seawater salmon in Chilean salmon industry. Antibiotic therapy is the first alternative to counteract infections caused by Piscirickettsia salmonis. The presence of bacterial biofilms on materials commonly used in salmon farming may be critical for understanding the bacterial persistence in the environment. In the present study, the CDC Biofilm Reactor® was used to investigate the effect of sub‐ and over‐MIC of florfenicol on both the pre‐formed biofilm and the biofilm formation by P. salmonis under the antibiotic stimuli on Nylon and high‐density polyethylene (HDPE) surfaces. This study demonstrated that FLO, at sub‐ and over‐MIC doses, decreases biofilm‐embedded live bacteria in the P. salmonis isolates evaluated. However, it was shown that in the P. salmonis Ps007 strain the presence of sub‐MIC of FLO reduced its biofilm formation on HDPE surfaces; however, biofilm persists on Nylon surfaces. These results demonstrated that P. salmonis isolates behave differently against FLO and also, depending on the surface materials. Therefore, it remains a challenge to find an effective strategy to control the biofilm formation of P. salmonis, and certainly other marine pathogens that affect the sustainability of the Chilean salmon industry.

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