Microbial Biofilm Proliferation within Sealer–Root Dentin Interfaces Is Affected by Sealer Type and Aging Period

Abstract

Background Root canal fillings are intended to prevent microbial proliferation over time in the canal after treatment. Objective To assess biofilm proliferation within the sealer-dentin interfaces of two methacrylate resin-based systems, self-etch (SE) and total-etch (TE), and an epoxy resin-based sealer (EP), aged for up to 6 months. Methods Standardized specimens (n=45) comprising the coronal 5 mm of human roots were filled with the test materials and gutta-percha. Specimens were either not pre-incubated (control; n=9), or incubated in sterile saline for 1 week, 1 month, 3 months or 6 months (n=3/group). Monospecies biofilms of Enterococcus faecalis were grown on the specimens for 7 days in a chemostat-based biofilm fermentor mimicking pathogenic oral conditions. The extent of E. faecalis proliferation within the sealer-dentin interface for each material and incubation period group was assessed using fluorescence microscopy of dihydroethidium-stained specimens. Results TE had less biofilm proliferation than both EP and SE (p<0.01). Deeper biofilm proliferation was detected in SE and EP specimens aged for 1 and 3 months than those aged for 1 week or 6 months (p<0.05). Maximum depth of biofilm penetration was recorded for SE at 1 month (p<0.05). Conclusion Within the test model used, the self-etch and epoxy resin-based sealers were more susceptible to interfacial biofilm proliferation than the total-etch restorative material. This susceptibility diminished after aging the materials’ interfaces for 6 months.

Adaptation and penetration of resin-based root canal sealers in root canals irradiated with high-intensity lasers

“…Many methods have been tried to accomplish complete disinfection and sealing of the root canal system. 1,2 Regarding the filling materials, the sealer must have a perfect adaptation to the dentin walls as well as the largest possible area of contact with dentin surfaces and penetrate into the dentin tubules. Studies using methacrylate resin-based sealers have shown promising results when compared with conventional sealers.…”

Biofilm Formation within the Interface of Bovine Root Dentin Treated with Conjugated Chitosan and Sealer Containing Chitosan Nanoparticles

“…Use of the chemostat-based biofilm fermentor in this study was based on our group's previously established non-destructive experimental model (21), which was subsequently adapted for assessment of the interface of root canal sealers with root dentin in simulation of intraoral pathogenic conditions (22). Unlike in the previous studies (24, 25), bovine roots were used in the current study, because their large canals facilitated standardized application of surface treatments with CSRB and PHCS.…”

“…Aged specimens were suspended in a chemostat-based biofilm fermentor to cultivate monospecies biofilms of Enterococcus faecalis (ATCC 29212) at 37° C (21, 22). Continuous flow of fresh Brain Heart Infused broth (BD Bioscience, Sparks, MD) was adjusted to 0.72L/d and dilution rate D = 0.075/hr to mimic the resting salivary flow rate (23).…”

Técnicas adesivas de obturação - Avaliação microscópica quantitativa da reparação apical e periapical pós-tratamento de canais radiculares de dentes de cães com lesão periapical

“…Uma maior proliferação Enterococcus faecalis, isoladamente, ou na forma de biofilme, foi detectada na interface parede de canal radicular e cimento Real Seal e cimento à base de resina amino-epóxica (AH Plus), quando comparados com uma resina fotopolorimerizável (Adaper Scotchbond Multi Purpose, 3M), em modelo experimental in vitro. Em ambos os canais radiculares obturados com Real Seal e AH Plus foi observada uma mais profunda proliferação de biofilme nós espécimes, após 1 e 3 meses, sendo a proliferação máxima observada com cimento Real Seal após período de um mês, tendendo a diminuir após 6 meses de avaliação (Roth et al, 2012). Em relação ao Real Seal, como utilizamos dentes com necrose pulpar e presença de lesão periapical, microrganismos remanescentes ao preparo biomecânico e curativo de demora, presentes nos túbulos dentinários, poderiam reingressar com mais facilidade ao canal radicular, e tecidos apicais e periapicais, sendo mais um fator que justificasse os resultados obtidos no nosso trabalho, à medida que uma tendência de diminuição do tamanho das lesões periapicais, menor prevalência de reabsorção óssea e radicular (50% dos casos) e selamento biológico parcial (30% dos casos), foram observados no período de 90 dias.…”