Microbial aerosol filtration: Growth and release of a bacteria–fungi consortium collected by fibrous filters in different operating conditions

Forthomme, Audrey; Joubert, Aurélie; Andrès, Yves; Simon, Xavier; Duquenne, Philippe; Bémer, Denis; Coq, Laurence Le

doi:10.1016/j.jaerosci.2014.02.004

Cited by 34 publications

(38 citation statements)

References 43 publications

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“…In fact, the F7 filter was 100% efficient for supermicronic particles at this level of clogging ( Figure 6). Forthomme et al (2014) also found similar values of filtration efficiency for cultivable S. epidermidis cells and cultivable P. oxalicum spores compared to inert particles (polyvinyl acetate).…”

Section: Microbial Aerosolssupporting

confidence: 60%

“…The microbial suspension was prepared according to the methodology of Forthomme et al (2014). It was composed of B. subtilis endospores and A. niger spores.…”

Section: Microbial Aerosolsmentioning

confidence: 99%

“…Each sample surface area was 25 cm 2 . An experimental protocol to extract microorganisms from filters was followed as presented by Forthomme et al (2014), Bonnevie Perrier et al (2008), Kemp et al (2001), and M€ oritz et al (1998). The filter sample was placed in an extraction solution composed of MgSO 4 (0.01M) and Tween 20 (0.25%).…”

Section: Microbial Concentration On Clogged Filtersmentioning

confidence: 99%

“…Studies at laboratory-scale have already focused on microbial growth and release on flat filters in different operating conditions (Bonnevie Perrier et al 2008;Forthomme et al 2014). It was found that factors such as the structural properties of filters, the nature of the collected particles, and the AHU operating conditions could influence microbial behavior on filters.…”

Section: Introductionmentioning

confidence: 99%

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Filtration performances of HVAC filters for PM10 and microbial aerosols— Influence of management in a lab-scale air handling unit

González

Joubert

Andrès

et al. 2016

Aerosol Science and Technology

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Filtration performances of air handling unit (AHU) filters for particles and microbial aerosols were investigated. The influence of the AHU operational conditions on the behavior of microorganisms collected on the filters was also studied. A lab-scale AHU with two filtration stages was developed and validated for the study of downsized filters with industrial geometries. Three types of filters of different efficiency were considered: G4, F7, and F9, according to European standard EN 779. Two configurations of filters were studied: G4 pleated/F7 bag and F7/F9 bag. Filters were sequentially clogged by alumina particles, which provided a mineral fraction in the particulate cake, and then by micronized rice particles, which provided the fungus Penicillium chrysogenum and an organic fraction that acts as a substrate for microorganisms. Finally, a microbial aerosol composed of endospores of Bacillus subtilis and spores of Aspergillus niger was nebulized to contaminate filters. After clogging, periods of 5 days on and 2 day weekend stops with restarts of ventilation were simulated for 6 weeks. The results showed that the filter efficiency for particles was quite comparable to that for microbial aerosols expressed in cultivable concentration. The particulate cake composed of alumina and micronized rice particles enabled the growth of the endogenous species P. chrysogenum and the survival of exogenous species B. subtilis and A. niger on filters. During restarts of ventilation, low particle concentrations were detected downstream of the second filtration stages by release but the microbial concentration from the fraction of air sampled was below the detection limit.

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Section: Microbial Aerosolssupporting

confidence: 60%

“…The microbial suspension was prepared according to the methodology of Forthomme et al (2014). It was composed of B. subtilis endospores and A. niger spores.…”

Section: Microbial Aerosolsmentioning

confidence: 99%

Section: Microbial Concentration On Clogged Filtersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Filtration performances of HVAC filters for PM10 and microbial aerosols— Influence of management in a lab-scale air handling unit

González

Joubert

Andrès

et al. 2016

Aerosol Science and Technology

Self Cite

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“…[28] wykazały, że w warunkach mikroklimatycznych sprzyjających wzrostowi drobnoustrojów -tj. w temperaturze 25°C i przy wilgotności względnej powietrza sięgającej maksimum (prawie 100%) -kontaminacja grzybami pleśniowy-mi materiału np.…”

Section: Omówienieunclassified

Fibers as carriers of microbial particles

Górny¹,

Ławniczek-Wałczyk²,

Stobnicka-Kupiec³

et al. 2015

Med Pr

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StreszczenieWstęp: Celem badania była ocena możliwości transportu cząstek biologicznych przez naturalne, syntetyczne i sztuczne włókna. Materiał i metody: Równoczesne pobieranie próbek pyłu osiadłego i aerozolu włóknistego przeprowadzono w 3 zakładach przetwarzających naturalne (bawełna, jedwab, len, konopie), syntetyczne (poliakrylonitrylowe, poliamidowe, polipropylenowe, poliestrowe) i sztuczne (wiskozowe) materiały włókniste; w 2 stajniach, gdzie hodowano konie i owce; w 4 domach, w których mieszkały psy i koty, oraz w 1 pawilonie zoologicznym dla lwów. Wszystkie próbki włókien analizowano laboratoryjnie, żeby okreś-lić stopień ich mikrobiologicznego zanieczyszczenia. Wyizolowane szczepy były identyfikowane jakościowo. W celu rozpoznania naturalnego upakowania i struktury badanych włókien, które mogą wspomagać transport cząstek biologicznych, przeprowadzono ich analizę skaningowymi mikroskopami elektronowymi. Wyniki: Zarówno osiadłe, jak i zawieszone w powietrzu włók-na transportowały takie same szczepy drobnoustrojów. Wszystkie włókna syntetyczne, sztuczne oraz włókna jedwabiu, które występują w środowisku jako osobne nici o gładkiej powierzchni, były wolne od zanieczyszczeń mikrobiologicznych. Naturalne włókna o luźnym upakowaniu i szorstkiej strukturze (np. runo owcy, sierść konia), o snopkowym upakowaniu i septowanej powierzchni (np. len, konopie) lub skręconych wstęgach i pofałdowanej strukturze (bawełna) transportowały do 9×10 5 jtk/g bakterii tlenowych, 3,4×10 4 jtk/g bakterii beztlenowych i 6,3×10 4 jtk/g grzybów, w tym patogenów klasyfikowanych przez Dyrektywę 2000/54/WE w 2. grupie zagrożenia. Wnioski: Ponieważ roślinne i zwierzęce włókna są zanieczyszczone znaczącą liczbą drobnoustrojów, w tym patogenów, powinny być mechanicznie eliminowane ze środowiska. Jeśli proces technologiczny na to pozwala, w zakładach pracy powinny być zastępowane włóknami syntetycznymi lub sztucznymi. W celu uniknięcia niepożądanej ekspozycji na szkodliwe czynniki mikrobiologiczne na włóknach zarówno w środowisku pracy, jak i poza nim powinny być wprowadzane środki techniczne, które wydajnie ograniczają obecność tych czynników. Med. Pr. 2015;66(4):511-523 Słowa kluczowe: zanieczyszczenie, środowisko pracy, bakterie, transport, grzyby, włókna Abstract Background: The aim of the study was to assess the ability of natural, synthetic and semi-synthetic fibers to transport microbial particles. Material and Methods: The simultaneously settled dust and aerosol sampling was carried out in 3 industrial facilities processing natural (cotton, silk, flax, hemp), synthetic (polyamide, polyester, polyacrylonitrile, polypropylene) and semi-synthetic (viscose) fibrous materials; 2 stables where horses and sheep were bred; 4 homes where dogs or cats were kept and 1 zoo lion pavilion. All samples were laboratory analyzed for their microbiological purity. The isolated strains were qualitatively identified. To identify the structure and arrangement of fibers that may support transport of microbial particles, a scanning electron microscopy ana...

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Bioaerosols: An Unavoidable Indoor Air Pollutant That Deteriorates Indoor Air Quality

Kallawicha

Chao

2022

Internet of Things

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Microbial aerosol filtration: Growth and release of a bacteria–fungi consortium collected by fibrous filters in different operating conditions

Cited by 34 publications

References 43 publications

Filtration performances of HVAC filters for PM10 and microbial aerosols— Influence of management in a lab-scale air handling unit

Filtration performances of HVAC filters for PM10 and microbial aerosols— Influence of management in a lab-scale air handling unit

Fibers as carriers of microbial particles

Bioaerosols: An Unavoidable Indoor Air Pollutant That Deteriorates Indoor Air Quality

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