Microbial activity on crude oil biomodification and its potential in facilitating distillation

Asshifa, M.N. Nur; Ibrahim, Mohamad Nasir Mohd; Yahya, Ahmad Shukri

doi:10.1016/j.jbiotec.2008.07.1141

Cited by 2 publications

(6 citation statements)

References 4 publications

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“…A wild‐type strain, Ps. aeruginosa USM‐AR2, was isolated and identified as a hydrocarbon‐utilizing and rhamnolipid‐producing bacterium (Nur Asshifa ; Nur Asshifa et al . ).…”

Section: Methodsmentioning

confidence: 99%

“…A production medium was used to cultivate Ps. aeruginosa USM‐AR2 containing the ingredients as follows per litre (Nur Asshifa ): 0·6% (w/v) yeast extract, 0·05% (w/v) MgSO 4 , 0·05% (v/v) Tween ® 80, 20 ml initial diesel. Diesel served as the sole carbon source and the feed substrate, while Tween ® 80 was added to increase diesel solubility in water and consequently assist in the diesel uptake by Ps.…”

Section: Methodsmentioning

confidence: 99%

“…A wild-type strain, Ps. aeruginosa USM-AR2, was isolated and identified as a hydrocarbon-utilizing and rhamnolipid-producing bacterium (Nur Asshifa 2009;Nur Asshifa et al 2012). A stirred-tank bioreactor was retrofitted with a foam collector on its gas outlet.…”

Section: Methodsmentioning

confidence: 99%

“…Typically, foam production in Ps. aeruginosa USM-AR2 culture is extremely quick and severe, making antifoam addition ineffective (Nur Asshifa 2009;Salwa et al 2010).…”

Section: Methodsmentioning

confidence: 99%

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Enhanced rhamnolipid production by Pseudomonas aeruginosa USM-AR2 via fed-batch cultivation based on maximum substrate uptake rate

Noh

Salleh

Yahya

2014

Lett Appl Microbiol

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Significance and Impact of the Study: This study highlights the significance of an effective fed-batch strategy for rhamnolipid production in a submerged fermentation using a water-immiscible substrate, based on maximum substrate uptake rate. The impact of this strategy ensured that the substrate was supplied at the rate matching the maximum substrate utilization by the cells without excess feeding, leading to increased rhamnolipid production, yield and productivity. AbstractA fed-batch strategy was established based on the maximum substrate uptake rate (MSUR) of Pseudomonas aeruginosa USM-AR2 grown in diesel to produce rhamnolipid. This strategy matches the substrate feed rates with the substrate demand based on the real-time measurements of dissolved oxygen (DO). The MSUR was estimated by determining the time required for consumption of a known amount of diesel. The MSUR trend paralleled the biomass profile of Ps. aeruginosa USM-AR2, where the MSUR increased throughout the exponential phase indicating active substrate utilization and then decreased when cells entered stationary phase. Rhamnolipid yield on diesel was enhanced from 0Á047 (g/g) in batch to 0Á110 (g/g) in pulse-pause fed-batch and 0Á123 (g/g) in MSUR fed-batch. Rhamnolipid yield on biomass was also improved from 0Á421 (g/g) in batch, 3Á098 (g/g) in pulse-pause fed-batch to 3Á471 (g/g) using MSUR-based strategy. Volumetric productivity increased from 0Á029 g l À1 h À1 in batch, 0Á054 g l À1 h À1 in pulse-pause fed-batch to 0Á076 g l À1 h À1 in MSUR fed-batch.

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“…A wild‐type strain, Ps. aeruginosa USM‐AR2, was isolated and identified as a hydrocarbon‐utilizing and rhamnolipid‐producing bacterium (Nur Asshifa ; Nur Asshifa et al . ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Typically, foam production in Ps. aeruginosa USM-AR2 culture is extremely quick and severe, making antifoam addition ineffective (Nur Asshifa 2009;Salwa et al 2010).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced rhamnolipid production by Pseudomonas aeruginosa USM-AR2 via fed-batch cultivation based on maximum substrate uptake rate

Noh

Salleh

Yahya

2014

Lett Appl Microbiol

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show abstract

“…In this study, Pseudomonas aeruginosa USM-AR2, a hydrocarbon-utilizing and a biosurfactant-producing bacterium (Nur Asshifa, 2009), was used to assist the conventional crude oil distillation process. Distillation is a common method for the fractionation of petroleum used in the laboratory as well as in refi neries, producing desirable petroleum-based products such as automotive fuel and kerosene (Kister, 1992).…”

mentioning

confidence: 99%

Rhamnolipid produced by <i>Pseudomonas aeruginosa</i> USM-AR2 facilitates crude oil distillation

Noh

Amirul

Ibrahim

et al. 2012

J. Gen. Appl. Microbiol.

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Microbial activity on crude oil biomodification and its potential in facilitating distillation

Cited by 2 publications

References 4 publications

Enhanced rhamnolipid production by Pseudomonas aeruginosa USM-AR2 via fed-batch cultivation based on maximum substrate uptake rate

Enhanced rhamnolipid production by Pseudomonas aeruginosa USM-AR2 via fed-batch cultivation based on maximum substrate uptake rate

Rhamnolipid produced by <i>Pseudomonas aeruginosa</i> USM-AR2 facilitates crude oil distillation

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