Rhamnolipid produced by &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; USM-AR2 facilitates crude oil distillation

Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel film. Streptomyces sp. PBD-311B was used for the biosynthesis of AgNPs. The synthesized bioAgNPs were characterized using UV-Vis spectroscopy, TEM, XRD, and FTIR analysis. Then, the bioAgNPs’ antibacterial and anticancer properties were determined using TEMA and cytotoxicity analysis. To form the antibacterial hydrogel film, bioAgNPs were mixed with a CNC and Alg solution and further characterized using FTIR analysis and a disc diffusion test. The average size of the synthesized bioAgNPs is around 69 ± 2 nm with a spherical shape. XRD analysis confirmed the formation of silver nanocrystals. FTIR analysis showed the presence of protein capping at the bioAgNP surface and could be attributed to the extracellular protein binding to bioAgNPs. The MIC value of bioAgNPs against P. aeruginosa USM-AR2 and MRSA was 6.25 mg/mL and 3.13 mg/mL, respectively. In addition, the bioAgNPs displayed cytotoxicity effects against cancer cells (DBTRG-0.5MG and MCF-7) and showed minimal effects against normal cells (SVG-p12 and MCF-10A), conferring selective toxicity. Interestingly, the bioAgNPs still exhibited inhibition activity when incorporated into CNC/Alg, which implies that the hydrogel film has antibacterial properties. It was also found that bioAgNP-CNC/Alg displayed a minimal or slow release of bioAgNPs owing to the intermolecular interaction and the hydrogel’s properties. Overall, bioAgNP-CNC/Alg is a promising antibacterial hydrogel film that showed inhibition against the pathogenic bacteria P. aeruginosa and MRSA and its application can be further evaluated for the inhibition of cancer cells. It showed benefits for surgical resection of a tumor to avoid post-operative wound infection and tumor recurrence at the surgical site.

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“…The fermentation media and conditions were adapted as in [64] with several modifications. First, glycerol stock of Streptomyces sp.…”

Section: Cultivation Of Streptomyces Sp Pbd-311bmentioning

confidence: 99%

Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using Streptomyces sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against P. aeruginosa USM-AR2 and MRSA

et al. 2021

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Biocatalytic upgrading of unconventional crude oil using oilfield-inhabiting bacterial consortia

Raheem

Hentati²,

Bahzad³

et al. 2022

International Biodeterioration & Biodegradation

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Study on Atmospheric Distillation of Some Plain and Chemically Dispersed Crude Oils: Comparison of Yields and Fuel Quality of Distillate Fractions

Ahmad¹,

Sohail²,

Khan³

et al. 2018

Energy Fuels

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This paper discusses the distillation behavior of the plain (control) and chemically dispersed crude oils. The oils used were paraffinic (denoted as P-RCP-I), naphthenic (denoted as P-RCN-II), and aromatic (denoted as P-RCA-III) crudes. The chemical dispersants used were anionic, cationic, and neutral surfactants including sodium dodecyl sulfate (SDS), cetyltrimethylammonium bromide (CTAB), and Triton X-100 (a branched p-octylphenol with an average of 9–10 ethylene oxide units). The effect of Dispersant-to-Oil ratio on the yields of distillate fractions (gasoline and diesel) was investigated. Looking at the results, we conclude that using chemically dispersed crudes enabled us to obtain better yields of light distillate fractions (F1 and F2) in comparison with the control. Among the dispersants used, the SDS was more effective in RCA-III crude where the yield of F1 increased significantly to 58% and that of residue decreased to 6%. The CTAB was found to be more effective in RCN-II crude, which gave a significant increase in yields of F1 to 49% with corresponding decrease in R yield, while the Triton X-100 also proved to be more effective in RCN-II crude which gave 48% and 6% yields of F1 and R fractions, respectively. The fuel properties of the resultant fractions derived from chemically dispersed crudes did not disturb and remained within the ranges prescribed for petrofuels.

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Rhamnolipid produced by <i>Pseudomonas aeruginosa</i> USM-AR2 facilitates crude oil distillation

Cited by 10 publications

References 25 publications

Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using Streptomyces sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against P. aeruginosa USM-AR2 and MRSA

Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using Streptomyces sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against P. aeruginosa USM-AR2 and MRSA

Biocatalytic upgrading of unconventional crude oil using oilfield-inhabiting bacterial consortia

Study on Atmospheric Distillation of Some Plain and Chemically Dispersed Crude Oils: Comparison of Yields and Fuel Quality of Distillate Fractions

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