Microbacterium hibisci sp. nov., isolated from rhizosphere of mugunghwa (Hibiscus syriacus L.)

Abstract

A Gram-stain-positive, aerobic, non-motile, short-rod shaped actinobacterium, designated THG-T2.14T, was isolated from soil sampled from the rhizosphere of mugunghwa. Growth occurred at 10-40 °C (optimum 30 °C), at pH 5.0-10.0 (optimum 7.0) and at 0-7.0 % NaCl (optimum 3.0 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T2.14T were identified as Microbacterium yannicii DSM 23203T (98.8 %), Microbacterium trichothecenolyticum DSM 8608T (98.8 %), Microbacterium arthrosphaerae DSM 22421T (98.7 %) and Microbacterium jejuense KACC 17124T (98.4 %). The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unidentified lipid, two unidentified phospholipids and two unidentified phosphoglycolipids. The menaquinones were MK-12, and MK-13. The major polyamine was spermidine. The peptidoglycan contained ornithine, alanine, glycine, homoserine and glutamic acid. The diagnostic diamino acid was ornithine. The acyl type of the muramic acid was glycolyl. The whole-cell sugars were rhamnose, ribose, galactose, arabinose, xylose and glucose. The DNA G+C content of strain THG-T2.14T was 71.2 mol%. The DNA-DNA relatedness between strain THG-T2.14T and its closest reference strains were significantly lower than the threshold value of 70 %. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T2.14T represents a novel species of the genus Microbacterium, for which the name Microbacterium hibisci sp. nov. is proposed. The type strain is THG-T2.14T (=KACC 18931T=CCTCC AB 2016180T).

Microbacterium paulum sp. nov., isolated from microfiltered milk

“…The genus was classified into the family Microbacteriaceae , order Microbacteriales , class Actinomycetia , and the type species is Microbacterium lacticum [3]. Microbacterium strains have been isolated from various environments, including air [4], compost [5, 6], faeces [7, 8], food [9], rhizosphere [10–12], seawater [13], sediment [14] and soil [15].…”

Bacillus suaedae sp. nov., isolated from the stem of Suaeda aralocaspica in north-west China

“…In the present study, fresh stem tissues, 1 g each, were washed and surface-sterilized as described by Yan et al . [12] and then ground to a slurry paste in 9 ml sterilized water by using a sterilized mortar and pestle. The obtained homogenate (200 µl) was coated on Reasoner’s 2A (R2A) agar medium (Difco; pH 9.0).…”

“…During an investigation on the endophyte diversity of S. aralocaspica located in the south edge of Gurbantunggut desert, Xinjiang, north-west China (44° 14′ N, 87° 42′ E) [8], strain YZJH907-2 T was isolated from the stem of the plant. In the present study, fresh stem tissues, 1 g each, were washed and surface-sterilized as described by Yan et al [12] and then ground to a slurry paste in 9 ml sterilized water by using a sterilized mortar and pestle. The obtained homogenate (200 µl) was coated on Reasoner's 2A (R2A) agar medium (Difco; pH 9.0).…”

Microbacterium caeni sp. nov., a novel species isolated from sludge in Yeosu, Korea

“…Catalase activity was tested with 3% H 2 O 2 , and oxidase activity was determined using tetramethyl-p-phenylenediamine, according to the methods described by Kovacs (1956). The optimum temperature range for growth of the NIBRBAC000506063 T strain was determined by cultivation in LB medium (BD) at different temperatures (4,10,15,18,25,30,37, 42, and 45°C) for 3 days. Salinity tolerance was determined on LB medium supplemented with NaCl (Sigma; 0, 0.5, 1, 2, 3, 4, 5, and 6%, w/v).…”