Microarray screening and qRT‐PCR evaluation of microRNA markers for forensic body fluid identification

Park, Jong‐Lyul; Park, Seong-Min; Kwon, Oh‐Seung; Lee, Han-chul; Kim, Jin‐Young; Seok, Hyun; Lee, Woo Sik; Lee, Seung Hwan; Kim, Yong‐Sung; Woo, Kwang-Man; Kim, Seon‐Young

doi:10.1002/elps.201400075

Cited by 63 publications

(37 citation statements)

References 37 publications

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“…Interestingly, miRNAs were not only tissue specific but also body fluid specific. MiR-1260b was first discovered to be specifically expressed in vaginal secretions [28]. The discrepancy of these studies might be derived from different tumor types and heterogeneity.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of miR-1260b in Non-small Cell Lung Cancer is Associated with Lymph Node Metastasis

Xu¹,

Li²,

Li³

et al. 2015

Aging and disease

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Lymph node (LN) metastasis is often an early event in the progression of malignant tumors and it contributes to the majority of cancer mortalities. MiRNAs play key roles in tumor metastasis. This study aimed to investigate the specific miRNAs as putative indicators of metastasis early diagnosis for non-small cell lung cancer (NSCLC). In this study, five NSCLC cases with LN metastasis and four cases without metastasis (NLN) were enrolled for Agilent Human miRNA array. The interested differentially expressed miRNA was validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in the LN metastasis (n = 46) and NLN (n = 39) groups. The microarray results revealed that three miRNAs (miR-1260b, miR-423-3p, miR-23a-5p) were differentially expressed in LN metastasis group compared with NLN group. The expression of miR-1260b was tested by qRT-PCR and the mean relative expression fold change (2 -ΔΔCt ) in LN metastasis was significantly higher than that in the NLN group (3.942, 1.743 respectively, P = 1.179E-04). The patients with tumor-node-metastasis (TNM) stage III were identified more frequently in LN metastasis group (P = 1.772E-11) and with a higher expression level of miR-1260b (5.126, P = 1.147E-06). In addition, the LN metastasis cases were associated with a poorly differentiated degree (P = 0.007). The overexpression of miR1260b in NSCLC with LN metastasis can be regarded as a specific signature for early progression and prognosis of NSCLC.

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Section: Discussionmentioning

confidence: 99%

Overexpression of miR-1260b in Non-small Cell Lung Cancer is Associated with Lymph Node Metastasis

Xu¹,

Li²,

Li³

et al. 2015

Aging and disease

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“…Wang et al [4] speculated that seminal miRNAs, as minimal invasive biomarkers, could provide useful information about gene expression in the male reproductive system. Therefore, seminal miRNAs possessed immense potential for forensic body fluid identification being expressed in a tissue specific manner and being less prone to degradation [5]. …”

Section: Introductionmentioning

confidence: 99%

Seminal miRNA Relationship with Apoptotic Markers and Oxidative Stress in Infertile Men with Varicocele

Mostafa

Rashed

Nabil

et al. 2016

BioMed Research International

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Aim. This study aimed to assess seminal miRNA relationship with seminal apoptotic markers and oxidative stress (OS) in infertile men associated with varicocele (Vx). Methods. In all, 220 subjects were divided into the following groups: fertile normozoospermic men, fertile normozoospermic men with Vx, infertile oligoasthenoteratozoospermic (OAT) men without Vx, and infertile OAT men with Vx. They were subjected to history taking, clinical examination, and semen analysis. In their semen, the following were estimated: miRNA-122, miRNA-181a, and miRNA-34c5 using quantitative real-time PCR, apoptotic markers (BAX, BCL2) protein expression, and OS markers [malondialdehyde (MDA) and glutathione peroxidase (GPx)]. Results. The mean levels of seminal miRNA-122, miRNA-181a, and miRNA-34c5 were significantly reduced in infertile OAT men with Vx compared with other groups coupled with Vx grade and Vx bilaterality. Seminal miRNA-122, miRNA-181a, and miRNA-34c5 were positively correlated with sperm concentration, total sperm motility, sperm normal morphology, seminal GPx, and seminal BCL2 and negatively correlated with seminal MDA and seminal BAX. Conclusions. Seminal miRNA-122, miRNA-181a, and miRNA-34c5 are decreased in infertile OAT men with Vx associated with increased Vx grade and Vx bilaterality. In addition, they are positively correlated with sperm parameters and negatively correlated with OS, apoptotic markers.

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“…Once isolated the miRNA is reverse transcribed, converting all of the miRNA in to cDNA. These cDNA libraries can be used as templates for quantitative (Real Time) Polymerase Chain Reaction (qPCR)to profile the miRNA [37]. miRNAs can be isolated and detected in bodily fluids such as plasma, saliva, semen and vaginal fluid [38].…”

Section: Mirnas As a Biomarker For Male Infertilitymentioning

confidence: 99%

Sperm-Specific MicroRNAs - Their Role and Function

Al-Gazi¹,

Carroll²

2016

GGS

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The sperm cell is highly specialised, with the sole purpose to fertilize an oocyte. Sperm assumes a simplistic structure, consisting of a head, which is mostly void of cytoplasm, a nucleus packed with DNA tightly wound around protamines; a midpiece housing the mitochondria necessary to fuel its locomotion, and a tail or principle piece that whips in motion to move the sperm towards the oocyte. However, despite the lack of cytoplasmic space, increasing studies in sperm biology have revealed that the sperm cell contains a myriad of molecular constituents that may play a role post-fertilization, influencing the oocyte and embryo development (figure 1). One such group of molecules are the small non-coding RNAs, which include the microRNAs (miRNAs). microRNAs miRNAs are small non-coding single-strand RNA molecules with sizes ranging from 17-26 nucleotides. The first miRNA to be discovered was lin-4 in the nematode, Caenorhabditis elegans. Lin-4 was detected with conserved complementary site on lin-14 and subsequently affected the larvae developmental timing by targeting mRNA and inhibiting its function [1]. Since then, miRNAs have emerged to play key roles in many biological functions through binding to 3'UTR (Untranslated Region) of specific mRNAs leading to either mRNA degradation or protein translation inhibition [2]. These miRNAs are involved in regulating cell functions including gene expression during development, differentiation, cell proliferation and apoptosis [3,4].In addition to having an influence in normal biological and developmental processes, miRNAs have been found to be involved in many pathogenic processes including Cardiovascular Diseases (CVD), cancer, inflammatory or autoimmune diseases, metabolic disorders and neurodegenerative disorders [5]. miRNA biogenesis miRNAs are encoded in the genome and transcribed by RNA polymerase II to form long primary miRNA transcripts (pri-miRNAs) that contain a cap structure at the 5' end and are poly-adenylated at the 3' end.The primary miRNAs are subsequently processed in the nucleus by Drosha to form shorter stem loop structures called pre curser-miRNAs or pre-miRNA (figure 2).These pre-miRNAs are then HSOA Journal of Genetics & Genomic Sciences Mini ReviewAbstract microRNAs (miRNAs) are small non-coding single-stranded molecules (containing about 22 nucleotides) that are widely found in many organisms, as varied as micro-organisms, plants, invertebrates and vertebrates. These miRNAs function in RNA silencing and post-transcriptional regulation of gene expression. miRNAs have been found to be involved in a number of biological and pathological processes, including sperm biology and infertility. This review will describe and discuss the role of miRNAs in terms of their role in sperm function, male infertility and gene regulation. a) The fertilising sperm introduces more than the paternal genome, the oocyte-activating factor (PLCζ), proteins from post-acrosomal, and perinuclear thecal proteins and centrioles; b) Sperm also contain a complex population of RNA, ...

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Microarray screening and qRT‐PCR evaluation of microRNA markers for forensic body fluid identification

Cited by 63 publications

References 37 publications

Overexpression of miR-1260b in Non-small Cell Lung Cancer is Associated with Lymph Node Metastasis

Overexpression of miR-1260b in Non-small Cell Lung Cancer is Associated with Lymph Node Metastasis

Seminal miRNA Relationship with Apoptotic Markers and Oxidative Stress in Infertile Men with Varicocele

Sperm-Specific MicroRNAs - Their Role and Function

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