Microarray profiling of differentially expressed lncRNAs and mRNAs in lung adenocarcinomas and bioinformatics analysis

He, Fei; Huang, Liping; Xu, Qiuping; Xiong, Weimin; Liu, Shuang; Yang, Huimin; Lu, Wenzhou; Xiao, Rendong; Hu, Zhijian; Cai, Lin

doi:10.1002/cam4.3369

Cited by 13 publications

(11 citation statements)

References 26 publications

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“…1 ). We further analyzed NK1R expression in NSCLC and adjacent non-tumor tissues in two datasets GSE130779 and GSE2514 downloaded from GEO databases [ 38 , 39 ], revealing that expression of NK1R was significantly upregulated in NSCLC tumor tissues compared with the adjacent non-tumor tissues or normal tissues (Fig. 1C ).…”

Section: Resultsmentioning

confidence: 99%

Neurokinin-1 receptor promotes non-small cell lung cancer progression through transactivation of EGFR

Zhang

et al. 2022

Cell Death Dis

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Despite the great advances in target therapy, lung cancer remains the top cause of cancer-related death worldwide. G protein-coupled receptor neurokinin-1 (NK1R) is shown to play multiple roles in various cancers; however, the pathological roles and clinical implication in lung cancer are unclarified. Here we identified NK1R as a significantly upregulated GPCR in the transcriptome and tissue array of human lung cancer samples, associated with advanced clinical stages and poor prognosis. Notably, NK1R is co-expressed with epidermal growth factor receptor (EGFR) in NSCLC patients’ tissues and co-localized in the tumor cells. NK1R can crosstalk with EGFR by interacting with EGFR, transactivating EGFR phosphorylation and regulating the intracellular signaling of ERK1/2 and Akt. Activation of NK1R promotes the proliferation, colony formation, EMT, MMP2/14 expression, and migration of lung cancer cells. The inhibition of NK1R by selective antagonist aprepitant repressed cell proliferation and migration in vitro. Knockdown of NK1R significantly slowed down the tumor growth in nude mice. The sensitivity of lung cancer cells to gefitinib/osimertinib is highly increased in the presence of the selective NK1R antagonist aprepitant. Our data suggest that NK1R plays an important role in lung cancer development through EGFR signaling and the crosstalk between NK1R and EGFR may provide a potential therapeutic target for lung cancer treatment.

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Section: Resultsmentioning

confidence: 99%

Neurokinin-1 receptor promotes non-small cell lung cancer progression through transactivation of EGFR

Zhang

et al. 2022

Cell Death Dis

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“…Tissue microarrays were performed as previously described. 23 Briefly, OPN antibody (1:200), vimentin antibody (1:100) and E‐cadherin antibody (1:100) were used to detect the protein expression of OPN, vimentin, and E‐cadherin. Additionally, the expression of OPN was semi‐quantitatively determined by two pathologists independently.…”

Section: Methodsmentioning

confidence: 99%

Regulatory roles of osteopontin in human lung cancer cell epithelial‐to‐mesenchymal transitions and responses

Shi

Hou

Wang

et al. 2021

Clinical & Translational Med

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“…Furthermore, LUAD and para-carcinoma tissue datasets were selected from the GEO database( https://www.ncbi.nlm.nih.gov/geo/ ). GSE85841 [ 33 ] (8 normal and 8 tumor samples) and GSE130779 [ 34 ] (8 normal and 8 tumor samples) were obtained. Results showed that logFC, logCPM, p value, and FDR of RIOX2 between the groups were 0.802107483, 3.681482308, 0.004666927, and 0.011185307, respectively.…”

Section: Resultsmentioning

confidence: 99%

The bioinformatics analysis of RIOX2 gene in lung adenocarcinoma and squamous cell carcinoma

Sun

Zhao

2021

PLoS ONE

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Lung cancer is characterized by high morbidity and mortality rates, and it has become an important public health issue worldwide. The occurrence and development of tumors is a multi-gene and multi-stage complex process. As an oncogene, ribosomal oxygenase 2 (RIOX2) has been associated with a variety of cancers. In this article, we analyzed the correlation between RIOX2 expression and methylation in lung cancer based on the databases including the cancer genome atlas (TCGA) (https://portal.gdc.cancer.gov/) and the gene expression omnibus (GEO) (https://www.ncbi.nlm.nih.gov/geo/). It was found that RIOX2 is highly expressed in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) tissues, whose expression is negatively correlated with its methylation level. In this regard, methylation at cg09716038, cg14773523, cg14941179, and cg22299097 had a significant negative correlation with RIOX2 expression in LUAD, whereas in LUSC, methylation at cg09716038, cg14773523, cg14941179, cg22299097, cg05451573, cg10779801, and cg23629183 is negatively correlated with RIOX2 expression. According to the analysis based on the databases, RIOX2 gene could not be considered as the independent prognostic biomarker in lung adenocarcinoma or squamous cell lung cancer. However, the molecular mechanism of RIOX2 gene in the development of lung cancer may be helpful in improving lung cancer therapy.

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Microarray profiling of differentially expressed lncRNAs and mRNAs in lung adenocarcinomas and bioinformatics analysis

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 13 publications

References 26 publications

Neurokinin-1 receptor promotes non-small cell lung cancer progression through transactivation of EGFR

Neurokinin-1 receptor promotes non-small cell lung cancer progression through transactivation of EGFR

Regulatory roles of osteopontin in human lung cancer cell epithelial‐to‐mesenchymal transitions and responses

The bioinformatics analysis of RIOX2 gene in lung adenocarcinoma and squamous cell carcinoma

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