Microarray profiling analysis and validation of novel long noncoding RNAs and mRNAs as potential biomarkers and their functions in atherosclerosis

Abstract

Long noncoding (lnc)RNAs have been implicated in the development and progression of atherosclerosis. However, the expression and mechanism of action of lncRNAs in atherosclerosis are still unclear. We implemented microarray analysis in human advanced atherosclerotic plaques and normal arterial intimae to detect the lncRNA and mRNA expression profile. Gene Ontology functional enrichment and pathway analyses were applied to explore the potential functions and pathways involved in the pathogenesis of atherosclerosis. A total of 236 lncRNAs and 488 mRNAs were selected for further Ingenuity Pathway Analysis. Moreover, quantitative RT-PCR tests of most selected lncRNAs and mRNAs with high fold changes were consistent with the microarray data. We also performed ELISA to investigate the corresponding proteins levels of selected genes and showed that serum levels of SPP1, CD36, ATP6V0D2, CHI3L1, MYH11, and BDNF were differentially expressed in patients with coronary heart disease compared with healthy subjects. These proteins correlated with some biochemical parameters used in the diagnosis of cardiovascular diseases. Furthermore, receiver operating characteristic analysis showed a favorable diagnostic performance. The microarray profiling analysis and validation of differentially-expressed lncRNAs and mRNAs in atherosclerosis not only provide new insights into the pathogenesis of this disease but may also reveal new biomarkers for its diagnosis and treatment.

Proteomics study on the effect of silybin on cardiomyopathy in obese mice

“…Therefore, they were selected for reverse transcription-quantitative PCR (RT-qPCR) verification. In addition, according to previous studies, MYH11 plays a regulatory role in cardiac function 26 , 27 . Furthermore, MYH11 was highly correlated with TPM1 and MYL2 in the PPI network of WS/WF and WF/WC.…”

LncRNA HCG11/miR‐26b‐5p/QKI5 feedback loop reversed high glucose‐induced proliferation and angiogenesis inhibition of HUVECs

“…Recent years lncRNAs have been implicated as potential biomarkers in the progression of antherosclerosis. [25][26][27] For instance, lncRNA Further reporter vectors construction and dual-luciferase reporter were implemented to verify the binding sites between miR-26b-5p…”

“…Recent years lncRNAs have been implicated as potential biomarkers in the progression of antherosclerosis 25‐27 . For instance, lncRNA MIAT aggravates atherosclerotic damage via activation PI3K/Akt signalling pathway, 10 LncRNA MANTIS played an crucial role in the beneficial effects of stain treatment and laminar flow 28 ; LncRNA CDKN2B‐AS1 decreased inflammatory response and promoted cholesterol efflux through inhibited the transcription of ADAM10 29 .…”

The Emerging Role of BDNF/TrkB Signaling in Cardiovascular Diseases

“…Likewise, studiesby Okada et alconfirmed that the plasma level of BDNF in myocardial ischemicmice increased significantly, which puts forward the central nervous system-mediated signal transduction mechanism to protect ischemic myocardium: when there is cardiac ischemic damage, myocardial cells through efferent nerve feedback to the central nervous system increase the expression of BDNF in the nerve cells, so as to increase the level of BDNF in peripheral blood, activating theangiogenesis-related molecular mechanism of inhibiting myocardial reconstruction and repair of ischemic myocardium [ 20 ]. Recently, a new study also uncovered serum BDNF was differentially expressed in plaque tissues in patients with coronary heart disease compared with normal intimae in healthy subjects using microarray profiling analysis and qRT-PCR validation [ 21 ]. Recent studies have revealed that BDNF regulates cardiomyocyte contraction and directly affects cardiac function of mice [ 22 , 23 ].…”