Proteomics study on the effect of silybin on cardiomyopathy in obese mice

Abstract

Due to the increase in the number of obese individuals, the incidence of obesity-related complications such as cardiovascular disease and type 2 diabetes is higher. The aim of the present study was to explore the effects of silybin on protein expression in obese mice. Firstly, serum was collected, and it was used to detect serum lipids and other serological indicators. Secondly, total protein from epididymal adipose tissue was extracted for differential expression analysis by quantitative tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectrometry (LC–MS/MS), followed by bioinformatics and protein–protein interaction (PPI) network analyses of these proteins. Lastly, real-time polymerase chain reaction (RT-PCR) and parallel reaction monitoring (PRM) were used to further validate the expression of identified differentially expressed proteins (DEPs) at the mRNA and protein level, respectively. The results revealed that silybin could improve abnormal lipid metabolism caused by the high fat diet in obese mice. A total of 341, 538 and 243 DEPs were found in the high fat/control (WF/WC), silybin/high fat (WS/WF) and WS/WC groups, respectively. These DEPs mainly participated in lipid metabolism and energy metabolism. Notably, tropomyosin 1 (TPM1), myosin light chain 2 (MYL2), myosin heavy chain 11 (MYH11) and other DEPs were involved in hypertrophic cardiomyopathy, dilated cardiomyopathy and other pathways. Silybin could protect cardiac function by inducing the protein expression of TPM1, MYL2 and MYH11 in the adipose tissue of obese mice.

CCND1 Overexpression in Idiopathic Dilated Cardiomyopathy: A Promising Biomarker?

“…Hydrolyzed proteins are involved in muscle contraction through adenosine triphosphate. Myocardial cytoskeleton proteins are important for maintaining the structural and functional integrity of the myocardium [ 46 ]. However, there are few studies on the changes in cytoskeletal proteins associated with dilated cardiomyopathy.…”

“…The generation of six isoforms, including VEGF111 , VEGF121 , VEGF145 , VEGF165 , VEGF189 , and VEGF206 , from VEGFA mRNA under the alternative splicing process has been reported [ 57 ]. VEGFA and its two receptors, VEGFR-1 and VEGFR-2 , play fundamental roles in angiogenesis under physiological and pathological conditions, as well as in neurological and cardiac growth and morphogenesis [ 46 , 58 ]. Disturbance in angiogenesis has been shown to contribute to the development of myocardial interstitial fibrosis in IDCM [ 59 , 60 , 61 , 62 ].…”

A Comprehensive Review of the Cardiovascular Protective Properties of Silibinin/Silymarin: A New Kid on the Block

“…Myosin Light Chain (2MYL-2) has been proven to play a crucial role in the normal development of ventricular cardiac myocyte structure and function [ 78 ]. Silibinin has been proven to increase the gene expression of these two genes [ 79 ]. The study that described this effect involved obese mice divided in two groups (High fat diet and High fat diet plus silibinin).…”

Silybin and its congeners: from traditional medicine to molecular effects

“…96 Finally, silybin (54 mg kg −1 , 4 weeks) improved abnormal lipid metabolism caused by a high-fat diet in obese mice, namely normalizing proteins involved in hypertrophic cardiomyopathy, dilated cardiomyopathy, and other pathways in the adipose tissue of obese mice. 97…”