Microarray profile of circular RNAs identifies hsa_circ_0014130 as a new circular RNA biomarker in non-small cell lung cancer

Abstract

Accumulating evidence has revealed that aberrant Circular RNAs (circRNAs) expression plays important roles in carcinogenesis and tumor progression. However, their role in non-small cell lung cancer (NSCLC) remains unclear. In this study, we first used circRNA microarrays to screen for tumour-specific circRNA candidates in between NSCLC (n = 3) and adjacent lung (n = 3) tissue. Among the circRNA expression profile, two circRNAs (hsa_circ_0014130 and hsa_circ_0016760) were selected for validation in ten pairs of NSCLC and adjacent non-cancerous tissues by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Only hsa_circ_0014130 exhibited significantly overexpressed in NSCLC tissues (P < 0.001), which were further confirmed in another 36 matched tissue samples using qRT-PCR. Hsa_circ_0014130 expression significantly correlated with TNM stage (P = 0.001) and lymphatic metastasis (P = 0.004). The area under the receiver operating characteristic curve was 0.878 (95% confidence interval = 0.804–0.951; P < 0.001), which showed good diagnostic potential. Bioinformatics platforms predicted that hsa_circ_0014130 might interact with five miRNAs and their corresponding mRNAs. Gene oncology analysis and pathway analysis revealed that hsa_circ_0014130 could participate in NSCLC development. In summary, our findings indicated that hsa_circ_0014130 could be used as a potential NSCLC biomarker and might be closely related to the carcinogenesis of NSCLC.

RNA-seq reveals the circular RNA and miRNA expression profile of peripheral blood mononuclear cells in patients with rheumatoid arthritis

“…There are many previous studies showing that lncRNAs participate in the pathogenesis of RA and can be regarded as biomarkers for the diagnosis of RA [16,17]. Recently, there is increasing evidence proving that circRNAs are widely involved in various physiological and pathological processes, including the occurrence and development of many diseases, including lung cancer [18], liver cancer [19], gynecological diseases [20] and more. Little research about the role of circRNAs in RA has been conducted.…”

Circular RNA circPIP5K1A promotes non‐small cell lung cancer proliferation and metastasis through miR‐600/HIF‐1α regulation

“…Previous pathway and gene oncology analyses revealed that circPIP5K1A is involved in development of NSCLC, although the underlying regulatory mechanism remains unknown . The circPIP5K1A cyclization was performed with exon 5 of the PIP5K1A gene, and the mature sequence that spliced was 724 bp (Figure A).…”

“…Previous pathway and gene oncology analyses revealed that circPIP5K1A is involved in development of NSCLC, although the underlying regulatory mechanism remains unknown. 15 The circPIP5K1A cyclization was performed with exon 5 of the PIP5K1A gene, and the mature sequence that spliced was 724 bp ( Figure 1A). qRT-PCR illustrated that circPIP5K1A expression increased in the PC9, A549, H1975,H1299, and H1650 NSCLC cell lines compared with the normal lung epithelial cell line BEAS-2B ( Figure 1B).…”

“…The circRNAs function importantly in tumor progression and carcinogenesis in NSCLC . The circRNA hsa_circ_0014130 (circPIP5K1A) was identified as a novel biomarker that is highly overexpressed in tissues of NSCLC comparing with adjacent tissues . However, the underlying regulatory mechanism remains unclear.…”

“…[12][13][14] The circRNA hsa_circ_0014130 (circPIP5-K1A) was identified as a novel biomarker that is highly overexpressed in tissues of NSCLC comparing with adjacent tissues. 15 However, the underlying regulatory mechanism remains unclear. Thus, the current investigation aimed to elucidate the function of circPIP5K1A.…”