Circular RNA expression profile of spleen in a Clostridium perfringens type C‐induced piglet model of necrotizing enteritis

Abstract

Clostridium perfringens type C is a pathogen that causes necrotizing enteritis ( NE ), which is an intestinal tract disease in piglets. The pathogenesis of C. perfringens type C‐induced NE is still unclear, leading to a lack of effective therapies. Earlier studies have reported that circular RNA s (circ RNA s) are involved in the pathogenic processes of various diseases. However, it is not known if circ RNA s in spleen play a role in C. perfringens type C infection in NE . To address this question, we infected 7‐day‐old piglets with C. perfringens type C to induce NE . Hematoxylin and eosin staining of small intestine revealed inflammation, atrophy and shedding of intestinal villi, and intestinal mucosal necrosis. We observed increased expression of cytokine genes (such as IL ‐1β and IL ‐6 ) and inflammation in the spleen. In addition, we used RNA ‐seq and bioinformatics analysis to examine changes in circ RNA expression. A total of 103 circ RNA s were found to be differentially expressed in NE , and Gene Ontology analysis revealed that the genes producing differentially expressed circ RNA s were enriched in regulation of the cellular metabolic process protein binding. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the genes producing differentially expressed circ RNA s were involved in the tumor necrosis factor signaling pathway, T cell receptor signaling pathway and nuclear factor‐κB signaling pathway. Finally, we found eight circ RNA s (including circ_0002220 and circ_0000821) that are related to NE . Therefore, our study provides new insights into the mechanisms underlying C. perfringens type C infection in piglets.

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

“…In pigs, Liang et al (2017) found that 21.93% of circRNAs in intergenic regions and 68.40% in exon regions through carrying out nine organs. Yan et al (2018) demonstrated that the found circRNAs consisted of approximately 74.31% exonic, 20.36% intergenic, and 5.33% intronic circRNAs in spleen, and Huang et al (2018) demonstrated that more than 86% of circRNAs consisted of exons while nearly 10% originated from intronic and intergenic regions in liver. In rats, Yang et al (2019) found that the circRNAs consisted of approximately 80.18% exonic, 0.15% intergenic, and 19.67% intronic circRNAs in pulmonary.…”

Genome-wide analysis of differentially expressed profiles of mRNAs, lncRNAs and circRNAs in chickens during Eimeria necatrix infection

“…Echinococcus granulosus, Cryptosporidium parvum, Cryptosporidium baileyi) in animals and humans [28,33,[66][67][68][69][70][71][72][73]. The expression profiles of host circRNAs were also elucidated after infections of avian leukosis virus J [74], bovine viral diarrhea virus [75], human cytomegalovirus [76], Orf virus [30], porcine endemic diarrhea virus [29], HIV [77], transmissible gastroenteritis virus [78], Mycobacterium tuberculosis [79], Clostridium perfringens [80] and C. baileyi [33]. Furthermore, the pivotal regulatory roles of some lncRNAs (e.g.…”

Exploration of Long Non-coding RNAs and Circular RNAs in Porcine Milk Exosomes

“…Interestingly, the differentially expressed lncRNA (ALDBSSCG0000001631) involved in ileum immune response in CPID was found in porcine milk exosome. They also reported the potential lncRNA ( Yan et al, 2018a ) and circRNA ( Yan et al, 2018b ) functions in the spleen of diarrheic piglets caused by CPID. Liu et al (2018) and Chen et al (2019) revealed the potential role of lncRNA or circRNA in muscle and fat development in piglets.…”