Microarray analysis reveals difference in gene expression profiles of hair and wool sheep infected with Haemonchus contortus

MacKinnon, Kathryn M.; Burton, Jeanne L.; Zajac, Anne M.; Notter, D. R.

doi:10.1016/j.vetimm.2009.02.013

Cited by 63 publications

(48 citation statements)

References 61 publications

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“…A number of previous studies have been undertaken to characterise the duodenal (Diez-Tascon et al, 2005;Keane et al, 2007;Keane et al, 2006), abomasal mucosal (Knight et al, 2011;Rowe et al, 2009) and lymph node and lymph fluid transcriptome (Andronicos et al, 2010;Gossner et al, 2013;Knight et al, 2010;MacKinnon et al, 2009) and have led to the identification of genes and biological processes associated with the host response to GIN. As a result of these studies, a number of pathways have been postulated to be involved in the development of a resistant phenotype; however, no clear consensus has emerged.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling of the ovine abomasal lymph node reveals a role for timing of the immune response in gastrointestinal nematode resistance

McRae

Good

Hanrahan

et al. 2016

Veterinary Parasitology

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Section: Introductionmentioning

confidence: 99%

Transcriptional profiling of the ovine abomasal lymph node reveals a role for timing of the immune response in gastrointestinal nematode resistance

McRae

Good

Hanrahan

et al. 2016

Veterinary Parasitology

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“…Host immunity against helminth infections is dependent on innate and acquired immune responses (STEAR & WAKELIN, 1998) and the interaction of these responses confers protection by inducing eosinophilic inflammation, mastocytosis and activation of Th2 responses, with increased expression of interleukin (IL) -4, IL-5 and IL-13 in target tissues and production of IgE and IgA (MACKINNON et al, 2009(MACKINNON et al, , 2010. In contrast, animals susceptible to infection develop Th1 responses, with secretion of cytokines such as IL-2, interferon-gamma (IFNG) and tumor necrosis factor (GILL et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Souza

Lambert

Nishi

et al. 2015

Rev. Bras. Parasitol. Vet.

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Galectins and collectins are proteins classified in the lectin family that have the ability to recognize molecular patterns associated with pathogens. Studies on cattle have demonstrated high expression of these proteins during infection with gastrointestinal nematodes. The aim of this study was to investigate whether the level of Haemonchus contortus infection would alter the expression of galectins (Gal11 and Gal14) and collectins (SPA and CGN) in sheep. Twelve Corriedale sheep exposed to natural infection with nematodes were divided into two groups: group 1 (G1, n = 7) and group 2 (G2, n = 5), with low and high parasite burdens, respectively, based on fecal egg counts and abomasal parasite counts. The fecal egg counts and abomasal parasite counts were significantly different (p < 0.05) between the groups. Galectin and collectin gene expression was observed in all sheep abomasal samples. However, animals with lower infection levels showed lower expression of the genes Gal14, SPA and CGN (p < 0.05). Expression of lectins was associated with the abomasal H. contortus burden, thus suggesting that these proteins may have a role in controlling of this infection.Keywords: Innate immunity, lectins, helminths, small ruminants, PCR. ResumoColectinas e galectinas são proteínas da família das lectinas que possuem a capacidade de reconhecer padrões moleculares associados aos patógenos. Estudos em bovinos têm demonstrado a alta expressão dessas proteínas durante a infecção por nematoides gastrintestinais. O objetivo deste estudo foi investigar se o nível de infecção de Haemonchus contortus altera a expressão de colectinas (SPA e CGN) e galectinas (Gal11 e Gal14) de ovinos. Doze ovinos da raça Corriedale expostos a infecção natural com nematoides foram separados em dois grupos: grupo 1 (G1, n=7) com menor grau de parasitismo; e grupo 2 (G2, n=5) com maior grau, a partir da contagem do número de parasitos recuperados do abomaso e OPG. A contagem de OPG e de parasitos recuperados do abomaso dos grupos G1 e G2 apresentaram diferença estatística (p<0,05). A expressão dos genes de colectinas e galectina foi observada em todas as amostras de abomaso dos ovinos, porém animais com menor grau de infecção apresentaram menor expressão dos genes de Gal14, SPA e CGN (p<0,05). A expressão de lectinas foi associada ao número de H. contortus encontrados no abomaso de ovinos, indicando um possível papel dessas proteínas no controle da infecção.Palavras-chave: Imunidade inata, lectinas, helmintos, pequenos ruminantes, PCR.

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“…In livestock, microarrays have been used to evaluate the skeletal muscle of pigs (Jiang et al, 2010 and and cattle (Lehnert et al, 2005;Wang et al, 2009). In sheep, the main studies were designed to evaluate the resistance to gastrointestinal parasites using microarrays with bovine cDNA (Mackinnon et al, 2009;Diez-Tascon, 2005) or ovine cDNA (Keane et al, 2006). Following analysis of gene expression by microarray, Vuocolo et al (2007) proposed a model depicting the regulatory network and the main epigenetic modifications likely to explain the changes in fibre types and the hypertrophy that characterises the Callipyge phenotype.…”

Section: Introductionmentioning

confidence: 99%

Differentially transcribed genes in skeletal muscle of lambs

et al. 2012

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a b s t r a c tThe objective of this study was to compare gene transcription profiles in Longissimus dorsi muscle of the following four hair sheep genetic groups, Morada Nova (MO), Brazilian Somali (SO), Santa Inês (SI) and ½Dorper Â ½Morada Nova (F1). These groups all display different postnatal muscle growth. The transcriptomes of the skeletal muscle of the lambs (at 200 days of age) were profiled by using oligonucleotide microarrays and reverse transcriptionquantitative real-time PCR (RT-qPCR). The microarray experiment identified 262 transcripts that were differentially expressed when transcription levels were compared between the different breeds. A total of 23 transcripts among which those involved in skeletal muscle development (MyoD1 and IGFBP4), lipogenesis and adipogenesis (C/EBPd, PPARg and PGDS) were differentially expressed in at least in one comparison. Clustering analysis showed that there is greater similarity in gene expression between the MO and SI breeds and between F1 and SO genetic groups. The SO breed has the most distinct expression pattern. The RT-qPCR results confirmed the findings from the microarray study. A positive correlation was observed between the expression of MyoD1 and the cold carcass yield. The negative correlations between the weight and yield of cold carcass with the expression of C/EBPd mean that the selection for adipogenesis could lead to a lower carcass weight. The GLUT3 and PYGL gene transcripts were negatively correlated with fat thickness, but ATP5G1 was positively correlated with this trait. Interestingly, many genes negatively correlated with PUFA were positively correlated with cold carcass yield. In conclusion, the present work demonstrated that there are breed-specific expression patterns in Brazilian hair sheep genetic groups. The differences in gene expression among genetic groups were consistent with their phenotypic differences. The positive correlation of the MyoD1 expression with the cold carcass yield suggests that this gene is important for tissue growth in sheep. The positive correlation of the C/EBPd expression with PUFA provides an opportunity to select for lipid deposition in meat animals.

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Microarray analysis reveals difference in gene expression profiles of hair and wool sheep infected with Haemonchus contortus

Cited by 63 publications

References 61 publications

Transcriptional profiling of the ovine abomasal lymph node reveals a role for timing of the immune response in gastrointestinal nematode resistance

Transcriptional profiling of the ovine abomasal lymph node reveals a role for timing of the immune response in gastrointestinal nematode resistance

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Differentially transcribed genes in skeletal muscle of lambs

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