Microarray analysis of radiation response genes in primary human fibroblasts

Kis, Enikő; Szatmári, Tünde; Keszei, Márton; Farkas, Róbert; Ésik, Olga; Lumniczky, Katalin; Falus, András; Sáfrány, Géza

doi:10.1016/j.ijrobp.2006.08.004

Cited by 121 publications

(104 citation statements)

References 49 publications

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“…In contrast, p53 appears to be required for the majority of the transcriptional response to IR in the early drosophila embryo (20). The extent of the contribution of p53 to the transcriptional response to IR in mammalian tissues varies (no doubt in part because of variation in experimental design) (21,22), and other transcription factors also play major roles (23,24). However, p53 clearly regulates the critical choice between apoptosis, cell-cycle arrest, and cell-cycle progression.…”

Section: Discussionmentioning

confidence: 99%

Suppressor of gamma response 1 ( SOG1 ) encodes a putative transcription factor governing multiple responses to DNA damage

Yoshiyama¹,

Conklin

Huefner

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

240

335

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The Arabidopsis sog1-1 (suppressor of gamma response) mutant was originally isolated as a second-site suppressor of the radiosensitive phenotype of seeds defective in the repair endonuclease XPF. Here, we report that SOG1 encodes a putative transcription factor. This gene is a member of the NAC domain [petunia NAM (no apical meristem) and Arabidopsis ATAF1,2 and CUC2] family (a family of proteins unique to land plants). Hundreds of genes are normally up-regulated in Arabidopsis within an hour of treatment with ionizing radiation; the induction of these genes requires the damage response protein kinase ATM, but not the related kinase ATR. Here, we find that SOG1 is also required for this transcriptional up-regulation. In contrast, the SOG1-dependent checkpoint response observed in xpf mutant seeds requires ATR, but does not require ATM. Thus, phenotype of the sog1-1 mutant mimics aspects of the phenotypes of both atr and atm mutants in Arabidopsis, suggesting that SOG1 participates in pathways governed by both of these sensor kinases. We propose that, in plants, signals related to genomic stress are processed through a single, central transcription factor, SOG1.Arabidopsis ͉ ATM ͉ ATR ͉ checkpoint response ͉ XPF

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Section: Discussionmentioning

confidence: 99%

Suppressor of gamma response 1 ( SOG1 ) encodes a putative transcription factor governing multiple responses to DNA damage

Yoshiyama¹,

Conklin

Huefner

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

240

335

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show abstract

“…Changes in the mRNA expression levels were reported by cDNA microarray data targeting radiation-responsive genes in human breast cancer cells (17)(18)(19)(20)(21). In addition, Kis et al identified radiation-responsive genes using a microarray analysis in primary human fibroblasts (22). These authors detected approximately 200 ionizing radiation (IR) responsive genes at the transcriptional level, of which 30 (28 up-and 2 downregulated) responded to radiation in all investigated cells and 20 were grouped according to function: DNA damage response (GADD45A, BTG2, PCNA and IER5), regulation of the cell cycle and cell proliferation (CDKN1A, PPM1D, Protein expression pattern in response to ionizing radiation in MCF-7 human breast cancer cells SERTAD1, PLK2, PLK3 and CYR61), programmed cell death (BBC3 and TP53INP1), signaling pathways (SH2D2A, SLIC1, GDF15, and THSD1), and other functions (SEL10, FDXR, CYP26B1 and OR11A1) (22).…”

Section: Introductionmentioning

confidence: 99%

Protein expression pattern in response to ionizing radiation in MCF-7 human breast cancer cells

Jung

Lee

et al. 2011

Oncology Letters

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Abstract. Breast cancer is one of the most common types of cancer in women and is highly treatable by radiotherapy. However, repeated exposure to radiation results in tumor cell resistance. Understanding the molecular mechanisms involved in the response of tumors to γ-irradiation is important for improving radiotherapy. For this reason, we aimed to identify radiation-responsive genes at the protein level. In the present study, we observed differentially expressed proteins using 2D-PAGE and MALDI-TOF-MS for the global analysis of protein expression patterns in response to ionizing radiation (IR). When the expression patterns of proteins were compared to a control gel, numerous spots were found that differed greatly. Among them, 11 spots were found to be significantly different. One set of proteins (GH2, RGS17, BAK1, CCNH, TSG6, RAD51B, IGFBP1 and CASP14) was upregulated and another set of proteins (C1QRF, PLSCR2 and p34 SE1-1 ) was downregulated after exposure to γ-rays. These proteins are known to be related to cell cycle control, apoptosis, DNA repair, cell proliferation and other signaling pathways.

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“…With a better understanding of radiation induced molecular processes, it might become possible to identify the radiosensitivity of individuals before the start of radiation therapy, leading to individualization of radiation treatment. Radiation-induced transcriptional responses have been studied using DNA microarray (Kis et al 2006;Jen and Cheung, 2006). Some previous studies have also examined cells harboring mutant p53 using DNA microarray (Amandson et al 2003; Scian et al 2004), but they did not examine each type of mutation.…”

Section: Discussionmentioning

confidence: 99%