Microarray analysis of placental tissue in intrauterine growth restriction

Struwe, Ellen; Berzl, Gabriele M.; Schild, R. L.; Blessing, Holger; Drexel, L.; Hauck, Brad; Tzschoppe, Anja; Weidinger, M.; Sachs, Martin M.; Scheler, C; Schleußner, E; Dötsch, Jörg

doi:10.1111/j.1365-2265.2009.03659.x

Cited by 58 publications

(44 citation statements)

References 34 publications

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“…In mice, hypermethylation in as few as one paternally expressed imprinted gene is sufficient to induce FGR (Murphy et al 2001, McMinn et al 2006, Dilworth et al 2010. Additionally, global methylation changes and altered gene expression in non-imprinted genes have also been identified in FGR placentas (McCarthy et al 2007, Einstein et al 2010, Struwe et al 2010.…”

Section: Introductionmentioning

confidence: 94%

Paternal obesity in a rodent model affects placental gene expression in a sex-specific manner

et al. 2015

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Fetal growth restriction (FGR) is a major obstetric complication stemming from poor placental development. We have previously demonstrated that paternal obesity in mice is associated with impaired embryo development and significantly reduced fetal and placental weights. We hypothesised that the FGR observed in our rodent model of paternal diet-induced obesity is associated with alterations in metabolic, cell signalling and stress pathways. Male C57BL/6 mice were fed either a normal or high-fat diet for 10 weeks before sperm collection for IVF and subsequent embryo transfer. On embryonic day 14, placentas were collected and RNA extracted from both male and female placentas to assess mRNA expression of 24 target genes using custom RT-qPCR arrays. Peroxisome proliferator-activated receptor alpha (Ppara) and caspase-12 (Casp12) expression were significantly altered in male placentas from obese fathers compared with normal (P!0.05), but not female placentas. PPARA and CASP12 proteins were localised within the placenta to trophoblast giant cells by immunohistochemistry, and relative protein abundance was determined by western blot analysis. DNA was also extracted from the same placentas to determine methylation status. Global DNA methylation was significantly increased in female placentas from obese fathers compared with normal (P!0.05), but not male placentas. In this study, we demonstrate for the first time that paternal obesity is associated with changes in gene expression and methylation status of extraembryonic tissue in a sex-specific manner. These findings reinforce the negative consequences of paternal obesity before conception, and emphasise the need for more lifestyle advice for prospective fathers.

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Section: Introductionmentioning

confidence: 94%

Paternal obesity in a rodent model affects placental gene expression in a sex-specific manner

et al. 2015

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“…Recent studies have focused on indices of fetal blood sampling (e.g. acidosis), abnormal fetal blood flow indices, cortisol and leptin levels in cord or neonatal blood, and modified insulin sensitivity testing [11][12][13].…”

Section: Importance Of Feeding In the First Periods Of Life: The Pretmentioning

confidence: 99%

Ghrelin and feeding behaviour in preterm infants

Savino¹,

Lupica²,

Liguori³

et al. 2012

Early Human Development

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a b s t r a c t a r t i c l e i n f o Available online xxxx Keywords: Ghrelin Feeding behaviour Preterm infantThe importance of early life events in the development of metabolic diseases is well recognized. Early postnatal environment, including nutrition, is key to future health, and this is particularly true for preterm infants. It is important that these infants receive sufficient nutrients to prevent growth restriction and promote neurodevelopment, while minimizing predisposition to metabolic diseases later in life. Feeding habits are the fundamental elements of nutrition and are influenced by many factors, including personal and familial habits, socioeconomic status, and cultural environment. In the last decades, there has been an important scientific interest toward the comprehension of the molecular and neural mechanisms regulating appetite. In these networks, act many peptide hormones produced in brain or gut, among which ghrelin is important because of its action in the short-term regulation of food intake and the long-term regulation of body weight. Ghrelin stimulates appetite and plays a role in regulating feeding behaviour. Ghrelin levels vary from fetal life through to early adulthood, with the highest levels observed in the very early years. Cord ghrelin levels have been evaluated in term and preterm newborns and high ghrelin levels have been observed in smallfor-gestational age newborns and in newborns with intrauterine growth restriction. Moreover, ghrelin has been detected in term and preterm human breast milk, suggesting that it may play a role in the development of neuroendocrine pathways regulating appetite and energy homeostasis in early life. However, more research is required to better define ghrelin's role in breast milk and on feeding behaviour.

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“…Notably, it has been suggested that leptin resistance could be programmed prenatally. 74 Genetic polymorphisms of ADRB3 (beta-3-adrenergic receptor), known for its involvement in lipolysis and thermogenesis in adipocytes, are associated with birthweight in animals and obesity in humans. 75,76 Impaired function of SLC2A3/ GLUT3 (solute carrier family 2A3/glucose transporter 3), which is involved in fetoplacental growth and metabolism, has been linked with intrauterine growth restriction and adiposity in animal models.…”

confidence: 99%