Microarray analysis of differential gene expression in sensitive and resistant pig to<i>Escherichia coli</i>F18

Bao, Wenbin; Ye, L.; Pan, Zhangyuan; Zhu, Junyan; Du, Zi-Dong; Zhu, Guoqiang; Huang, Xiaoliang; Wu, Shenglong

doi:10.1111/j.1365-2052.2011.02287.x

Cited by 33 publications

(27 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the paired full-sib individuals that were selected from an established resource of Sutai pigs (Duroc x Meishan) that were characterized as resistant or sensitive to E. coli F18, our research team identified the expression profiles of some genes, including TAP1, in duodenum. The results showed that TAP1 played a vital role in resisting E. coli F18 infection (Bao et al, 2012).…”

Section: Introductionmentioning

confidence: 85%

Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance

Wang¹,

Liu²,

Dong³

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The transporter associated with antigen processing (TAP) transports peptides from the cytosol into the endoplasmic reticulum for subsequent loading onto the major histocompatibility complex (MHC) class I molecules. This study showed the dynamic changes in the TAP1 expression level in newborn to weaning piglets. Tissue expression profiles revealed that the TAP1 gene was expressed at low levels in all tissues, and the expression levels were relatively higher in the lung, spleen, lymph, and thymus; further, no significant difference was observed in the expression in each tissue among the 3 unweaned stages (8, 18, and 30 days). Nevertheless, the postweaning (35 days) expression levels in tissues, including the spleen, lung, lymph, duodenum, and jejunum were significantly higher than those in the unweaned stages. Furthermore, gene ontology and pathway analysis showed that TAP1 took part in 38 biological functions and 5 pathway processes, including ABC transporters and antigen processing and presentation. These analyses showed that the TAP1 gene, which was 3687 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 3686-3692 (2014) TAP1 gene related to Escherichia coli F18 resistance related to MHC I immune regulation, had a stable and low expression level in unweaned stages; however, its expression increased in the postweaning stages. The high expression level of TAP1 indicated that the gene might play an important role in Escherichia coli F18 resistance.

show abstract

Section: Introductionmentioning

confidence: 85%

Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance

Wang¹,

Liu²,

Dong³

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…Mammalian TLRs constitute a large family of transmembrane receptors, of which TLR4 has received the most attention and has been found to induce the production of inflammatory factors and cytokines during anti-infection and immunity processes (Takeda and Akira 2005, Zacharowski et al 2006. We previously used Agilent two-color microarray-based gene expression profiling to investigate differential gene expression, and found that the TLR4 signaling pathway may regulate resistance to E. coli F18 in weaned piglets (Bao et al 2012). Meishan pigs, a popular Chinese local breed, were infected with E. coli F18 and examination of intestinal tissue, bacterial counts and binding assays revealed two distinct resistant and sensitive phenotypes (Wu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Differential expression of Toll-like receptor 4 signaling pathway genes in Escherichia coli F18-resistant and – sensitive Meishan piglets

Y¹,

Ln²,

Wy³

et al. 2016

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The Toll-like receptor 4 (TLR4) signaling pathway is an important inflammatory pathways associated with the progression of numerous diseases. The aim of the present study was to investigate the relationship between TLR4 signaling and resistance to Escherichia coli F18 in locally weaned Meishan piglets. Using a real-time PCR approach, expression profiles were determined for key TLR4 signaling pathway genes TLR4, MyD88, CD14, IFN-α, IL-1β and TNF-α in the spleen, thymus, lymph nodes, duodenum and jejunum of E. coli F18-resistant and -sensitive animals. TLR4 signaling pathway genes were expressed in all the immune organs and intestinal tissues, and the expression was generally higher in the spleen and lymph nodes. TLR4 transcription was higher in the spleen of sensitive piglets (p<0.05), but there was no significant difference in TLR4 mRNA levels in other tissues. Similarly, CD14 transcription was higher in lymph nodes of sensitive animals (p<0.05) but not in other tissues. IL-1β expression was higher in the spleen and in the duodenum of resistant piglets (p<0.05, p<0.01, respectively), and there were no significant differences in other tissues. There were also no significant differences in the expression of MyD88, TNF-α and IFN-α between sensitive and resistant piglets (p>0.05). These results further confirm the involvement of the TLR4 signaling pathway in resistance to E. coli F18 in Meishan weaned piglets. The resistance appeared to be mediated via downregulation of TLR4 and CD14, and upregulation of MyD88 that may promote the release of cytokines TNF-α, IL-1β, IFN-α and other inflammatory mediators which help to fight against E. coli F18 infection.

show abstract

“…This study was conducted in 3 E. coli F18-resistant piglets and 3 E. coli F18-susceptible piglets (Bao et al, 2012). All experiments were conducted in the Animal Hospital of Yangzhou University according to the regulations of the Administration of Affairs Concerning Experimental Animals (Ministry of Science and Technology, China, revised in June 2012) and approved by experimental animal using permit with No.…”

Section: Differential Expression Of Porcine Bpi Gene In E Coli F18-rmentioning

confidence: 99%

Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility

Liu

Dong

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recombinant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escherichia coli, and 293-6E cells by gel electrophoresis and Western blotting. Escherichia coli F18 is the primary Gram-negative bacteria in the gut and the main pathogen leading to diarrhea and edema disease in weaning piglets. Therefore, E. coli F18-resistant and -sensitive Sutai piglets were used to test differential expression of BPI protein by Western blotting and to investigate the potential correlation between BPI protein expression and E. coli F18-susceptibility. Recombinant porcine BPI protein expression was not detected in the prokaryotic and yeast expression systems; however, soluble protein was detected in the eukaryotic expression system. These data indicate the strong bacterio- static action of the BPI protein and confirm the feasibility of obtaining large amounts of recombinant porcine BPI recombinant protein using this eukaryotic expression system. In addition, the BPI protein expression levels in the E. coli F18-resistant group were significantly higher than those in the sensitive group, indicating that high BPI protein expression is associated with resistance to E. coli F18. Our findings provide a basis for further investigations into the development of a drug designed to confer resistance to E. coli F18 in weaning piglets.

show abstract

Microarray analysis of differential gene expression in sensitive and resistant pig toEscherichia coliF18

Cited by 33 publications

References 24 publications

Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance

Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance

Differential expression of Toll-like receptor 4 signaling pathway genes in Escherichia coli F18-resistant and – sensitive Meishan piglets

Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility

Contact Info

Product

Resources

About