Microangiopathie diabétique: étude comparative chez l’homme et l’animal1

Duhault, J; Lebon, Florence; Boulanger, Mathilde; Regnault, F; Kern, Francis

doi:10.3233/bir-1974-11303

Cited by 5 publications

(6 citation statements)

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“…This is in contrast to reports on retinopathy in KKmice where a decreased frequency of mural cells has been reported (Duhault et al 1973(Duhault et al , 1974. Also in retinopathy in rats (Leuenberger et al 1971(Leuenberger et al , 1974Cohen et al 1972) and in humans (Cogan et al 1961;;Kuwabara & Cogan 1963a;de Oliveira 1966;Yanoff 1972) disappearance of mural cells has been noted.…”

Section: Retinal Cafillaries In Obese-hyperglycemic Micecontrasting

confidence: 76%

Morphology and Enzyme Activities of the Retinal Capillaries in Mice With the Obese‐hyperglycaemic Syndrome (Gene Symbol Ob)

Naeser

Ågren

1978

Acta Ophthalmologica

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The retinal capillary bed from 67 obese-hyperglycaemic mice and 64 lean litter mates was isolated by trypsin digestion and investigated with respect to structure and enzyme activities. There was no significant difference in the ratio between numbers of endothelial and mural cells. The capillary walls did not show any obvious structural differences and microaneurysms were not observed. The retinal vessels from the obese-hyperglycaemic mice, however, displayed significantly higher activities of the enzymes hydroxyacyl-CoA-dehydrogenase, asparate aminotransferase (ASAT) and adenylate kinase than their lean litter mates. The activities of glutathione reductase, glucose-6-phosphate dehydrogenase (G-6-PDH) and phosphofructokinase were similar in the two experimental groups. It is suggested that the present data reflect early metabolic disturbances related to diabetic retinopathy.

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Section: Retinal Cafillaries In Obese-hyperglycemic Micecontrasting

confidence: 76%

Morphology and Enzyme Activities of the Retinal Capillaries in Mice With the Obese‐hyperglycaemic Syndrome (Gene Symbol Ob)

Naeser

Ågren

1978

Acta Ophthalmologica

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“…This increase in the rate of biosynthesis and processing offibronectin and the increase of collagen type III (5) appears to be accompanied by a decreased rate of synthesis and/or increased rate of removal of heparan sulfate proteoglycans in diabetic basal lamina (ref. These perturbations of the biosynthesis of extracellular matrix macromolecules result in the increased thickness and permeability of diabetic basement membranes (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). The increased capillary permeability may also enable plasma fibronectin to diffuse in increased amounts to the extracellular matrix (19).…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

“…The increased thickness of capillary basement membranes is one of the histologically and electron-microscopically detectable manifestations of this perturbed matrix biosynthesis (6)(7)(8). It has been proposed that the deranged regulation of extracellular matrix biosynthesis concerns most if not all mesenchymal cells, not only those involved in basement membrane deposition (1)(2)(3)(4)(5)(6)(7)(8)(9). Biosynthetic experiments performed with biopsy specimens of diabetic human conjunctiva and skin, with biopsy specimens from diabetic (KK strain) mouse conjunctiva, and with diabetic (streptozotocin-induced) rat conjunctiva showed an increased rate of incorporation of [14C]proline into polymeric (insoluble) collagen (ref.…”

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confidence: 99%

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Increased biosynthesis and processing of fibronectin in fibroblasts from diabetic mice.

Phan‐Thanh¹,

Robert²,

Derouette³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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Diabetic connective tissues exhibit a deranged regulation of extracellular matrix biosynthesis. Fibronectin is shown to be increased in human dermal connective tissue by immunofluorescence, mainly at the dermoepidermal and capillary basement membranes. The rate of fibronectin biosynthesis, excretion, and incorporation in a pericellular polymeric form was investigated using genetically diabetic KK mouse skin and fibroblasts as compared to Swiss and C57BL mouse skin and fibroblasts. The rate of incorporation of [mS]methionine into proteins recovered in the culture medium or in deoxycholate and NaDodSO4 or urea extracts was investigated. The rate of incorporation in the medium and deoxycholate extracts was comparable. However, the relative rate of incorporation of the tracer in the NaDodSO4-extractable, pericellular polymeric form was increased in the diabetic KK fibroblasts both for total proteins and for fibronectin. In pulse-chase experiments, the deoxycholate-soluble and NaDodSO4-soluble fractions exhibited a precursor-product relationship. The rate of passage of fibronectin from the deoxycholate-soluble (cellular compartment) form to the NaDodSO4-soluble (pericellular polymeric) form was strongly accelerated in the diabetic fibroblast cultures. These results confirm the increased rate of synthesis of fibronectin in diabetic fibroblasts as well as its processing from the cellular compartment to the polymeric pericellular form. The increase of fibronectin in diabetic connective tissues, in the matrix as well as in the basement membranes, may play a role in the mechanism of micro-and macroangiopathies and in the perturbed permeability characteristics of the diabetic capillaries, and as a glycoprotein it may contribute to the increased periodic acid/Schiff reagent staining of diabetic capillary basement membranes.The diabetic state is accompanied by deranged regulation of the biosynthesis of extracellular matrix macromolecules (1-5). The increased thickness of capillary basement membranes is one of the histologically and electron-microscopically detectable manifestations of this perturbed matrix biosynthesis (6)(7)(8). It has been proposed that the deranged regulation of extracellular matrix biosynthesis concerns most if not all mesenchymal cells, not only those involved in basement membrane deposition (1-9). Biosynthetic experiments performed with biopsy specimens of diabetic human conjunctiva and skin, with biopsy specimens from diabetic (KK strain) mouse conjunctiva, and with diabetic (streptozotocin-induced) rat conjunctiva showed an increased rate of incorporation of [14C]proline into polymeric (insoluble) collagen (ref. 1, pp. 314-323; refs. 4-9). In these same experiments, an increased rate of incorporation of glucosamine into connective tissue glycoproteins was also seen (ref. 1, pp. 314-323; refs. 3-9). Experiments performed on genetically diabetic (KK strain) mouse skin explants showed an increased rate of incorporation of ["4C]proline into type III collagen, with no increase of total collagen...

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“…Decrease in the number of mural pericytes in diabetic retinopathy has been reported in human diabetes (Cogan et al 1961;de Oliveira 1966;Yanoff 1972) and in experimental (Leuenberger et al 1971(Leuenberger et al , 1974Cohen et al 1972;Studer et al 1975;Papachristodoulou et al 1976), and spontaneous animal diabetes (Gepts & Touissaint 1967;Duhault et al 1973Duhault et al , 1974.…”

Section: Discussionmentioning

confidence: 93%

Morphology and Enzyme Activities of the Retinal Capillaries in Streptozotocin‐diabetic Mice

Ågren

Rehn

Naeser

1979

Acta Ophthalmologica

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Male lean mice belonging to the obese-hyperglycemic strain were made diabetic by intravenous injection of streptozoticin. The retinal capillary bed freed by trypsin digestion was studied with regard to morphology and the activity of some enzymes. There was a significant increase in the ratio between the endothelial and mural cells which was interpreted as indicating mural pericyte disappearance. The activities of adenylate kinase, aspartate-aminotransferase and hydroxyacyl-CoA-dehydrogenase in the retinal vessels of the diabetic animal were significantly higher than in vessels from the control animals. No differences were found in the activities of glucose-6-phosphate dehydrogenase, glutathione reductase and phosphofructokinase between the two animal groups. It is suggested that these results reflect early morphological and metabolic changes of the retinal vessels, preceding the well known clinical picture of diabetic retinopathy.

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Microangiopathie diabétique: étude comparative chez l’homme et l’animal1

Cited by 5 publications

References 0 publications

Morphology and Enzyme Activities of the Retinal Capillaries in Mice With the Obese‐hyperglycaemic Syndrome (Gene Symbol Ob)

Morphology and Enzyme Activities of the Retinal Capillaries in Mice With the Obese‐hyperglycaemic Syndrome (Gene Symbol Ob)

Increased biosynthesis and processing of fibronectin in fibroblasts from diabetic mice.

Morphology and Enzyme Activities of the Retinal Capillaries in Streptozotocin‐diabetic Mice

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