Microanatomy of Adult Zebrafish Extraocular Muscles

Kasprick, Daniel S.; Kish, Phillip E.; Junttila, Tyler L.; Ward, Lindsay; Bohnsack, Brenda L.; Kahana, Alon

doi:10.1371/journal.pone.0027095

Cited by 13 publications

(12 citation statements)

References 28 publications

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“…The remaining nIII subpopulations, IR and MR motoneurons, both innervate ipsilateral extraocular muscles, and are both labeled in Tg(isl1:GFP). Furthermore, both the IR and MR muscles are found near the dye insertion site (Kasprick et al, ). Thus, we were unable to distinguish IR from MR motoneurons in our fills.…”

Section: Resultsmentioning

confidence: 99%

Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio

Greaney

Privorotskiy

D’Elia

et al. 2016

J of Comparative Neurology

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Both spatial and temporal cues determine the fate of immature neurons. A major challenge at the interface of developmental and systems neuroscience is to relate this spatiotemporal trajectory of maturation to circuit‐level functional organization. This study examined the development of two extraocular motor nuclei (nIII and nIV), structures in which a motoneuron's identity, or choice of muscle partner, defines its behavioral role. We used retro‐orbital dye fills, in combination with fluorescent markers for motoneuron location and birthdate, to probe spatial and temporal organization of the oculomotor (nIII) and trochlear (nIV) nuclei in the larval zebrafish. We describe a dorsoventral organization of the four nIII motoneuron pools, in which inferior and medial rectus motoneurons occupy dorsal nIII, while inferior oblique and superior rectus motoneurons occupy distinct divisions of ventral nIII. Dorsal nIII motoneurons are, moreover, born before motoneurons of ventral nIII and nIV. The order of neurogenesis can therefore account for the dorsoventral organization of nIII and may play a primary role in determining motoneuron identity. We propose that the temporal development of extraocular motoneurons plays a key role in assembling a functional oculomotor circuit. J. Comp. Neurol. 525:65–78, 2017. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.

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Section: Resultsmentioning

confidence: 99%

Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio

Greaney

Privorotskiy

D’Elia

et al. 2016

J of Comparative Neurology

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“…High magnification images disclosed that jam‐b2 was specifically expressed in lines around and between the eyes in a pattern that matched muscle‐specific MF20 antibody staining, suggesting that jam‐b2 is expressed in EOMs and jaw muscles (Fig. B) (Kasprick et al, ). However, the specific expression in EOMs and jaw muscles disappeared after 96 hpf, indicating that jam‐b2 is expressed transiently during development and differentiation of these muscles (Fig.…”

Section: Resultsmentioning

confidence: 94%

Zebrafish jam‐b2 Gal4‐enhancer trap line recapitulates endogenous jam‐b2 expression in extraocular muscles

Matsui

Dorigo

Buchberger

et al. 2015

Developmental Dynamics

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Background: Members of the junctional adhesion molecule (JAM) family function as cell adhesion molecules and cell surface receptors. The zebrafish genome contains six different jam genes, and jam‐b and jam‐c were shown to be essential for myoblast fusion during skeletal muscle development. However, little is known about jam‐b2 expression and function. Results: We isolated the cDNA of zebrafish jam‐b2. jam‐b2 is expressed specifically in extraocular muscles (EOMs), jaw muscles, and pectoral fins in zebrafish larvae, but not in trunk muscles. The identified jam‐b2 expression pattern is supported by the analysis of a zebrafish Gal4‐enhancer trap line, in which the coding sequence of the transcriptional activator KalTA4 together with a Gal4‐dependent UAS‐mCherry expression cassette was inserted into the jam‐b2 locus. Intercrosses with an UAS:EGFP strain proves the possibility for targeting transgene expression to EOMs, jaw muscles and fins. Finally, we characterized the concerted contraction pattern of EOMs in larvae performing an optokinetic response. Conclusions: The expression pattern of jam‐b2 suggests that it may contribute different properties to EOMs, jaw muscles, and pectoral fins. The jam‐b2:KalTA4‐UAS‐mCherry transgenic strain serves a dual role as both a reporter for these muscles and as a valuable genetic tool for targeting transgene expression to EOMs. Developmental Dynamics 244:1574–1580, 2015. © 2015 Wiley Periodicals, Inc.

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“…The remaining nIII subpopulations, IR and MR motoneurons, both innervate ipsilateral extraocular muscles, and are both labeled in Tg(isl1:GFP). Furthermore, both the IR and MR muscles are found near the dye insertion site (Kasprick et al, 2011). Thus, we were unable to distinguish IR from MR motoneurons in our fills.…”

Section: Resultsmentioning

confidence: 99%

Ocular Motoneuron Pools Develop Along a Dorsoventral Axis in Zebrafish,Danio rerio

Greaney

Privorotskiy

D’Elia

et al. 2016

Preprint

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Both spatial and temporal cues determine the fate of immature neurons. A major challenge at the interface of developmental and systems neuroscience is to relate this spatiotempo-ral trajectory of maturation to circuit-level functional organization. This study examined the development of two ocular cranial motor nuclei (nIII and nIV), structures in which a motoneuron’s identity, or choice of muscle partner, defines its behavioral role. We used retro-orbital dye fills, in combination with fluorescent markers for motoneuron location and birth-date, to probe spatial and temporal organization of the oculomotor (nIII) and trochlear (nIV) nuclei in the larval zebrafish. We described a dorsoventral organization of the four nIII motoneuron pools, in which inferior and medial rectus motoneurons occupy dorsal nIII, while inferior oblique and superior rectus motoneurons occupy distinct divisions of ventral nIII. Dorsal nIII motoneurons are, moreover, born before motoneurons of ventral nIII and nIV. Order of neurogenesis can therefore account for the dorsoventral organization of nIII and may play a primary role in determining motoneuron identity. We propose that the temporal development of ocular motoneurons plays a key role in assembling a functional oculomotor circuit.

show abstract

Microanatomy of Adult Zebrafish Extraocular Muscles

Cited by 13 publications

References 28 publications

Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio

Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio

Zebrafish jam‐b2 Gal4‐enhancer trap line recapitulates endogenous jam‐b2 expression in extraocular muscles

Ocular Motoneuron Pools Develop Along a Dorsoventral Axis in Zebrafish,Danio rerio

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