Microanalytical Screening of All Major Stratum Corneum Lipids by Sequential High-performance Thin-layer Chromatography

Melnik, Bodo C.; Hollmann, J.; Erler, Elke; Verhoeven, Bernhard; Plewig, Gerd

doi:10.1111/1523-1747.ep12276757

Cited by 99 publications

(46 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The surrogate composition is based on the chemical composition of human stratum corneum. Human stratum corneum consists of keratin as the main structure-forming protein and the intercellular lipid bilayers comprising fatty acids, triglycerides, cholesterol, ceramides and sterolesters (Table 2) [22][23][24][25].…”

Section: Skin Surrogate Fabricationmentioning

confidence: 99%

Towards drug quantification in human skin with confocal Raman microscopy

Franzen

Selzer

Fluhr

et al. 2013

European Journal of Pharmaceutics and Biopharmaceutics

View full text Add to dashboard Cite

CitationTowards drug quantification in human skin with confocal Raman microscopy. AbstractUnderstanding the penetration behavior of drugs into human skin is a prerequisite for the rational development and evaluation of effective dermal drug delivery.The general procedure for the acquisition of quantitative drug penetration profiles in human skin is performed by sequential segmentation and extraction. Unfortunately, this technique is destructive, laborious and lacks spatial resolution. Confocal Raman microscopy bares the potential of a chemically selective, label free and nondestructive analysis. However, the acquisition of quantitative drug depth profiles within skin by Raman microscopy is impeded by imponderable signal attenuation inside the tissue.In this study, we present a chemical semi-solid matrix system simulating the optical properties of human skin. This system serves as a skin surrogate for investigation of Raman signal attenuation under controlled conditions. Caffeine was homogeneously incorporated within the skin surrogate and Raman intensity depth profiles were acquired. A mathematical algorithm describing the Raman signal attenuation within the surrogate was derived from these profiles. Human skin samples were incubated with caffeine and Raman intensity depth profiles were similarly acquired. The surrogate algorithm was successfully applied to correct the drug profiles in human skin for signal attenuation. For the first time, a mathematical algorithm was established which allows correction of Raman signal attenuation in human skin, thus facilitating reliable drug quantification in human skin by confocal Raman spectroscopy.Keywords: confocal Raman microscopy, human skin, quantitative depth profiling, dermal drug delivery, skin surrogate, Raman signal attenuation 3

show abstract

Section: Skin Surrogate Fabricationmentioning

confidence: 99%

Towards drug quantification in human skin with confocal Raman microscopy

Franzen

Selzer

Fluhr

et al. 2013

European Journal of Pharmaceutics and Biopharmaceutics

View full text Add to dashboard Cite

show abstract

“…The permeability barrier in the stratum corneum is provided by these lipid bilayers and corneocytes (Elias & Friend, 1975;Elias et al, 1981;Wertz & Downing, 1982;Landmann, 1986). In the stratum corneum the main classes of lipids are ceramides, cholesterol, free fatty acids and cholesterol-3-sulphate (Gray et al, 1982;Wertz & Downing, 1983;Wertz et al, 1985;Melnik et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Development of an optimal protocol for the ultrastructural examination of skin by transmission electron microscopy

Bergh

Swartzendruber

Geest

et al. 1997

Journal of Microscopy

View full text Add to dashboard Cite

The intercellular lipid bilayers of the stratum corneum provide the permeability barrier of the skin. To perform an electron microscopical examination of the ultrastructure of these bilayers, ruthenium tetroxide fixation is required. In this study an optimal fixation protocol was developed and selected upon comparing seven different fixation procedures, using glutaraldehyde (GA) and the postfixatives ruthenium red, osmium tetroxide (OsO4) and ruthenium tetroxide (RuO4) in combination with potassium ferrocyanide (K4Fe(CN)6) and potassium ferricyanide (K3Fe(CN)6). Instead of fixing skin with either OsO4 or RuO4, these two fixatives were combined in one protocol. In addition, the use of RuRed was introduced and the influence of both K4Fe(CN)6 and K3Fe(CN)6 in combination with RuO4 were examined. Furthermore, we compared two dehydration solvents, methanol and acetone. The most satisfying results were obtained when the skin was prefixed in GA and postfixed in OsO4 and RuO4 with K3Fe(CN)6, i.e. with Fe in its highest oxidation state (Fe3+). No differences were observed between dehydration in methanol and acetone.

show abstract

“…Densitometric signals were integrated with a Spectra Physics 4270 integrator. Further details have been described elsewhere [10]. The results were evaluated by Student’s t test on a level of significance of 0.05.…”

Section: Methodsmentioning

confidence: 99%

Lipid Composition of Outer Stratum corneum in Hereditary Palmoplantar Keratodermas

Küster¹,

Melnik

Traupe

et al. 2003

Dermatology

View full text Add to dashboard Cite

Background: The analysis of lipid composition of the outer stratum corneum is a promising approach to study the pathophysiology of inherited disorders of keratinization. Objective: The purpose of the study was the search for biochemical alterations of stratum corneum lipids in hereditary palmoplantar keratoderma (PPK). Methods: Using high-performance thin-layer chromatography, we performed an analysis of all major stratum corneum lipid classes in scales of 29 patients with 8 different types of hereditary PPK. Results: In comparison to the controls, slight differences in the lipid pattern were found in all keratodermas. Reduced amounts of total ceramides and increased levels of free fatty acids were noted in nearly all types. Conclusions: The study indicates that the abnormal composition of stratum corneum lipids in PPK is probably not caused by genetic defects of the epidermal lipid metabolism, but it appears to represent an epiphenomenon of a disturbed cornification.

show abstract

Microanalytical Screening of All Major Stratum Corneum Lipids by Sequential High-performance Thin-layer Chromatography

Cited by 99 publications

References 21 publications

Towards drug quantification in human skin with confocal Raman microscopy

Towards drug quantification in human skin with confocal Raman microscopy

Development of an optimal protocol for the ultrastructural examination of skin by transmission electron microscopy

Lipid Composition of Outer Stratum corneum in Hereditary Palmoplantar Keratodermas

Contact Info

Product

Resources

About