Microalgae disruption techniques for product recovery: influence of cell wall composition

Alhattab, Mariam; Kermanshahi-pour, Azadeh; Brooks, Marianne Su-Ling

doi:10.1007/s10811-018-1560-9

Cited by 149 publications

(66 citation statements)

References 176 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the chemical profile of, and the metabolic routes involving carbohydrates (mainly starch and cellulose) may differ significantly from species to species of microalga [139,140,141]. In particular, cell wall thickness and composition depend on microalgal species, growth conditions and stage of growth [142].…”

Section: Microalgae As Prebioticsmentioning

confidence: 99%

Potential Industrial Applications and Commercialization of Microalgae in the Functional Food and Feed Industries: A Short Review

2019

View full text Add to dashboard Cite

Bioactive compounds, e.g., protein, polyunsaturated fatty acids, carotenoids, vitamins and minerals, found in commercial form of microalgal biomass (e.g., powder, flour, liquid, oil, tablet, or capsule forms) may play important roles in functional food (e.g., dairy products, desserts, pastas, oil-derivatives, or supplements) or feed (for cattle, poultry, shellfish, and fish) with favorable outcomes upon human health, including antioxidant, anti-inflammatory, antimicrobial, and antiviral effects, as well as prevention of gastric ulcers, constipation, anemia, diabetes, and hypertension. However, scale up remains a major challenge before commercial competitiveness is attained. Notwithstanding the odds, a few companies have already overcome market constraints, and are successfully selling extracts of microalgae as colorant, or supplement for food and feed industries. Strong scientific evidence of probiotic roles of microalgae in humans is still lacking, while scarce studies have concluded on probiotic activity in marine animals upon ingestion. Limitations in culture harvesting and shelf life extension have indeed constrained commercial viability. There are, however, scattered pieces of evidence that microalgae play prebiotic roles, owing to their richness in oligosaccharides—hardly fermented by other members of the intestinal microbiota, or digested throughout the gastrointestinal tract of humans/animals for that matter. However, consistent applications exist only in the dairy industry and aquaculture. Despite the underlying potential in formulation of functional food/feed, extensive research and development efforts are still required before microalgae at large become a commercial reality in food and feed formulation.

show abstract

Section: Microalgae As Prebioticsmentioning

confidence: 99%

Potential Industrial Applications and Commercialization of Microalgae in the Functional Food and Feed Industries: A Short Review

2019

View full text Add to dashboard Cite

show abstract

“…A major deterrent in studying the lipid composition of the chloroplast is the technical challenge associated with the isolation and puri cation of intact chloroplasts. The currently used techniques employ physical or chemical means to break the cell wall and the plasmalemma [19,20]. The isolation techniques of chloroplast have been con ned to several higher plants and a model alga.…”

Section: Introductionmentioning

confidence: 99%

Isolation of chloroplasts from marine microalgae Isochrysis galbana Parke suitable for organelle lipid composition analysis

Ye¹,

Li²,

Xu³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Marine microalgae, Isochrysis galbana Parke, is an important diet microalgal species with high nutritional value. Different from other unicellular microalgae, its cell contains two chloroplasts which are the major sites for lipid synthesis. Results: Here, we optimized a chloroplast isolation approach suitable for I. galbana Parke, and evaluated the purity and integrity of the isolated chloroplasts by microscopic observations and biochemical assays. The chloroplast lipids were sequenced by ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Glycolipids were the main chloroplast lipids, and DGDG, MGDG, and SQDG were the most abundant glyceroglycolipids. DGMG and SQMG were not observed among the chloroplast lipids. In addition, DG was the most abundant neutral lipid. A part of fatty acyl R1/R2 with MGMGs, DGDGs, MGDGs, SQDGs, PEs, and PCs were not found in chloroplasts. The fatty acid proportion of chloroplast lipids were increased, decreased, or remained unchanged compared with the whole-cell. Conclusions: This newly developed isolation approach was a simple and reliable method to isolate chloroplasts with high integrity and purity. Collectively, our findings show that such an isolation approach may be used in studies on many different aspects of chloroplast biology, and offers a useful reference for isolation of chloroplast from other marine microalgae.

show abstract

“…1). The cell-wall is usually composed of polysaccharides, with small content of proteins and ash (Baudelet et al, 2017;Domozych et al, 2012;Lahaye and Robic, 2007) The rigidity of the cell-wall depends on the nature of the polysaccharides present and on their interactions with proteins and other molecules (Alhattab et al, 2018). Some strains contain a pseudo cell-wall rich in exopolysaccharide mucilage (Safi et al, 2014a), which can break easily at high shear forces.…”

Section: Algae Structurementioning

confidence: 99%

“…The morphological differences among algal strains and their connection to fractionation methods has not been thoroughly considered as a basis for the biorefinery. Several studies have addressed the influence of cell-wall composition on the disintegration and extraction performance (Alhattab et al, 2018;Baudelet et al, 2017;Safi et al, 2014c;Wang and Lan, 2018). However, the fractionation and purification steps are rarely covered.…”

Section: Cell-structure Dimensionmentioning

confidence: 99%

“…Also, in case of a mixed algae population, the operational parameters of the mill can be tuned to operate within a range of energy values (energy released in the milling chamber) to selectively release high value biomolecules that are produced by a certain strain only. A more extensive knowledge on the cell-wall composition and the energy required to disrupt a single cell is therefore needed(Alhattab et al, 2018).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Biorefinery of functional biomolecules from algae

García¹

View full text Add to dashboard Cite

The disintegration of three industry relevant algae (Chlorella vulgaris, Neochloris oleoabundans and Tetraselmis suecica) was studied in a lab scale bead mill at different bead sizes (0.3 mm-1 mm). Cell disintegration, proteins and carbohydrates released into the water phase followed a first order kinetics. The process is selective towards proteins over carbohydrates during early stages of milling. In general, smaller beads led to higher kinetic rates, with a minimum specific energy consumption of ≤ 0.47 kWh kgDW-1 for 0.3 mm beads. After analysis of the stress parameters (stress number and stress intensity), it appears that optimal disintegration and energy usage for all strains occurs in the 0.3-0.4 mm range. During the course of bead milling, the native structure of the marker protein Rubisco was retained, confirming the mildness of the disruption process.

show abstract

Microalgae disruption techniques for product recovery: influence of cell wall composition

Cited by 149 publications

References 176 publications

Potential Industrial Applications and Commercialization of Microalgae in the Functional Food and Feed Industries: A Short Review

Potential Industrial Applications and Commercialization of Microalgae in the Functional Food and Feed Industries: A Short Review

Isolation of chloroplasts from marine microalgae Isochrysis galbana Parke suitable for organelle lipid composition analysis

Biorefinery of functional biomolecules from algae

Contact Info

Product

Resources

About