Micro-stamped surfaces for the patterned growth of neural stem cells

Nanocomposite and Nanostructured Carbon-based Films as Growth Substrates for Bone Cells

“…For example, surfaces containing celladhesive microdomains of tetrafluoroethylene, placed on a cell-repulsive background of tetraethylene glycol dimethyl ether, were used for controlling the shape, degree of spreading, assembly of actin cytoskeleton, phenotypic modulation and proliferation of vascular smooth muscle cells (Goessl et al, 2001). Similarly, cell-repellent domains of poly(ethylene) oxide-like plasma-deposited films, combined with cell-adhesive poly-Llysine domains, were used for controlled adhesion, arrangement and neurite outgrowth of human neural stem cells derived from umbilical cord blood (Ruiz et al, 2008). In our earlier study (Filova et al, 2009), micropatterned surfaces were prepared by the successive plasma polymerization of acrylic acid (AA) and 1,7-octadiene (OD) through a metallic mask on the inner surface of 24-well polystyrene multidishes.…”

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

“…Many cell types have been successfully patterned with microfluidics [14][15][16], lCP [17], inkjet printing [18,19], plasma treatment [20], self-assembled monolayers [21][22][23][24], self-assembled constructs [25], laser scanning lithography [26], atomic force microscope lithography, dip-pen nanolithography [27], topography [28,29], carbon nano-tubes [30], or their combinations [31,32]. Neurons are, however, distinctive cells with highly polarized morphology, much smaller somata, and thus few anchoring points for adhesion in comparison to most types of adherent mammalian cells.…”

Surface Treatments to Modulate Bioadhesion: A Critical Review

“…One attractive aspect of micro/nano printing of polymers is that of introducing both topographical and surface chemistry changes into the material. This is evidenced by Ruiz et al [116] and Dusseiller et al [120] who showed that neural cell growth and epithelial cell growth can be manipulated with this technique, respectively. This technique has been advanced further by using proteins in the micro/nano contact printing [117][118][119] which allows for a more bio-functionalized surface to be developed.…”

“…This is on account of the fact that polymeric materials generally tend to be cheaper than metals and ceramics and can be seen in many instances to be easier to machine and manipulate to adapt the polymer for use in specific environments, especially biological environments. To this end, micro-and nano-printing has been developed and applied to polymeric materials [116][117][118][119][120][121]. A typical surface resulting from micro-contact printing can be seen in Figure 6.…”