Nanocomposite and Nanostructured Carbon-based Films as Growth Substrates for Bone Cells

Bačáková, Lucie; Grausova, Lubica; Vacı́k, J.; Kromka, Alexander; Biederman, Hynek; Choukourov, Andrei; Starý, V.

doi:10.5772/15105

Cited by 11 publications

(16 citation statements)

References 151 publications

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“…These samples had much lower wettability than the plasma-treated samples. This result is in accordance with the previous finding by ourselves and by other authors that carbon-based coatings, for example, with amorphous diamond-like carbon, graphite or nonfunctionalized fullerenes, are often hydrophobic (for a review, see [19]). Nevertheless, the wettability of C-modified LDPE in this study still remained higher than on pristine LDPE.…”

Section: Resultssupporting

confidence: 93%

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Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances

Pařízek

Kasálková

Bačáková

et al. 2013

BioMed Research International

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The attractiveness of synthetic polymers for cell colonization can be affected by physical, chemical, and biological modification of the polymer surface. In this study, low-density polyethylene (LDPE) was treated by an Ar+ plasma discharge and then grafted with biologically active substances, namely, glycine (Gly), polyethylene glycol (PEG), bovine serum albumin (BSA), colloidal carbon particles (C), or BSA+C. All modifications increased the oxygen content, the wettability, and the surface free energy of the materials compared to the pristine LDPE, but these changes were most pronounced in LDPE with Gly or PEG, where all the three values were higher than in the only plasma-treated samples. When seeded with vascular smooth muscle cells (VSMCs), the Gly- or PEG-grafted samples increased mainly the spreading and concentration of focal adhesion proteins talin and vinculin in these cells. LDPE grafted with BSA or BSA+C showed a similar oxygen content and similar wettability, as the samples only treated with plasma, but the nano- and submicron-scale irregularities on their surface were more pronounced and of a different shape. These samples promoted predominantly the growth, the formation of a confluent layer, and phenotypic maturation of VSMC, demonstrated by higher concentrations of contractile proteins alpha-actin and SM1 and SM2 myosins. Thus, the behavior of VSMC on LDPE can be regulated by the type of bioactive substances that are grafted.

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Section: Resultssupporting

confidence: 93%

“…In addition, conjugated double bonds between carbon atoms are created, and this renders the polymer surface electrically conductive. It is known that the electrical conductivity of a material surface enhances its attractiveness for cell colonization, even without active electrical stimulation (for a review, see [11–13, 19]). …”

Section: Introductionmentioning

confidence: 99%

Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances

Pařízek

Kasálková

Bačáková

et al. 2013

BioMed Research International

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“…These changes could lead not only to the modification of the chemical properties and the polarity of the material surface, but also to its enhanced nanoscale roughness. These factors together could result in the facilitation and enhancement of cell adhesion (for a review, see [14,33–35]). For example, substrates with nanoscale irregularities promote the adsorption of cell adhesion-mediating extracellular matrix molecules (e.g., fibronectin, vitronectin) present in the serum supplement of the culture media, in an appropriate geometrical conformation, which enables good accessibility of specific sites in these molecules ( i.e.…”

Section: Resultsmentioning

confidence: 99%

“…It has been repeatedly shown that the formation of oxygen-containing chemical functional groups on the material surface supports the cell adhesion (for a review, see [32–35]). However, on the fresh fullerene films, which contained more oxygen on their surface, the initial cell adhesion was poorer than on the aged film with a lower surface oxygen concentration.…”

Section: Resultsmentioning

confidence: 99%

Growth and Potential Damage of Human Bone-Derived Cells on Fresh and Aged Fullerene C60 Films

Kopová

Bačáková

Lavrentiev

et al. 2013

IJMS

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Fullerenes are nanoparticles composed of carbon atoms arranged in a spherical hollow cage-like structure. Numerous studies have evaluated the therapeutic potential of fullerene derivates against oxidative stress-associated conditions, including the prevention or treatment of arthritis. On the other hand, fullerenes are not only able to quench, but also to generate harmful reactive oxygen species. The reactivity of fullerenes may change in time due to the oxidation and polymerization of fullerenes in an air atmosphere. In this study, we therefore tested the dependence between the age of fullerene films (from one week to one year) and the proliferation, viability and metabolic activity of human osteosarcoma cells (lines MG-63 and U-2 OS). We also monitored potential membrane and DNA damage and morphological changes of the cells. After seven days of cultivation, we did not observe any cytotoxic morphological changes, such as enlarged cells or cytosolic vacuole formation. Furthermore, there was no increased level of DNA damage. The increasing age of the fullerene films did not cause enhancement of cytotoxicity. On the contrary, it resulted in an improvement in the properties of these materials, which are more suitable for cell cultivation. Therefore, fullerene films could be considered as a promising material with potential use as a bioactive coating of cell carriers for bone tissue engineering.

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“…DNPs are generally considered as materials with little, if any cytotoxicity. These nanoparticles dispersed in the cell culture media did not affect the adhesion, growth, viability and differentiation of various cell types (for a review, see [82,83]). For example, the addition of DNPs in the culture medium did not affect the adhesion, growth and adipogenic or osteogenic differentiation of human adipose tissue-derived stem cells [84].…”

Section: The Potential Cytotoxicity Of Diamond Nanoparticlesmentioning

confidence: 99%

The Application of Nanodiamond in Biotechnology and Tissue Engineering

Bačáková¹,

Brož²,

Lišková³

et al. 2016

Diamond and Carbon Composites and Nanocomposites

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Diamond in the allotrope form consists of carbon atoms arranged in a cubic crystal structure covalently bonded in sp 3 hybridization. Diamond has emerged as a very promising material for various biomedical applications due to its excellent mechanical properties (hardness, low friction coefficient, good adhesiveness to the underlying substrate, good interlayer cohesion), optical properties (the ability to emit intrinsic luminescence), electrical properties (good insulator in the pristine state and semiconductor after doping), chemical resistance (low chemical reactivity and resistance to wet etching) and biocompatibility (little if any toxicity and immunogenicity). For advanced biomedical applications, diamond is promising particularly in its nanostructured forms, namely nanoparticles, nanostructured diamond films and composite scaffolds in which diamond nanoparticles are dispersed in a matrix (mainly nanodiamond-loaded nanofibrous scaffolds). This chapter summarizes both our long-term experience and that of other research groups in studies focusing on the interaction of cells (particularly bone-derived cells) with nanodiamonds as nanoparticles, thin films and composites with synthetic polymers. Their potential applications in bioimaging, biosensing, drug delivery, biomaterial coating and tissue engineering are also reviewed.

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Nanocomposite and Nanostructured Carbon-based Films as Growth Substrates for Bone Cells

Cited by 11 publications

References 151 publications

Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances

Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances

Growth and Potential Damage of Human Bone-Derived Cells on Fresh and Aged Fullerene C60 Films

The Application of Nanodiamond in Biotechnology and Tissue Engineering

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