Micro-spherical cochleate composites: method development for monodispersed cochleate system

Nagarsekar, Kalpa; Ashtikar, Mukul; Steiniger, Frank; Thamm, Jana; Schacher, Felix H.; Fahr, Alfred

doi:10.3109/08982104.2016.1149865

Cited by 15 publications

(6 citation statements)

References 35 publications

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“…has also been suggested as potential aggregation inhibitors, but feasibility is uncertain [19]. A very recent comparison between a novel, microfluidics-based strategy and conventional cochleate production methods has underlined that aggregation prevails as a major, unsolved problem [20]. In the present work we developed an efficient method of preparing stable, disperse cochleates.…”

Section: Introductionmentioning

confidence: 99%

Dispersion and stabilization of cochleate nanoparticles

Bozó

Wacha

Mihály

et al. 2017

European Journal of Pharmaceutics and Biopharmaceutics

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Cochleates, calcium-stabilized membrane rolls of nanoscale diameter, promise a unique and efficient way of delivering lipid-soluble drugs, proteins or nucleic acids into biological systems because they protect the encapsulated material against enzymatic or chemical degradation. Self-aggregation, which typically arises during production and storage is a major obstacle that has so far precluded the development of an efficient cochleate-based drugdelivery system. Here we show that citric acid, added transiently in a narrow concentration range, effectively disperses cochleate aggregates, stabilizes the disperse state for long-term storage and preserves the canonical ultrastructure and topological characteristics of cochleate nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Dispersion and stabilization of cochleate nanoparticles

Bozó

Wacha

Mihály

et al. 2017

European Journal of Pharmaceutics and Biopharmaceutics

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“…Examples include lipid nanoparticles, 20) polymer nanoparticles, [21][22][23] non-ionic surfactant nanoparticles 24) and cochleate microparticles. 25) To date, most applications have focused on the preparation of liposomes [26][27][28] and there is little information regarding the generation of other types of nanoparticles using this approach. In this study, we focused on the preparation of PLGA nanoparticles and investigated the influence of various conditions on the PLGA nanoparticles.…”

Section: )mentioning

confidence: 99%

The Use of an Efficient Microfluidic Mixing System for Generating Stabilized Polymeric Nanoparticles for Controlled Drug Release

Morikawa

Tagami

Hoshikawa

et al. 2018

Biological & Pharmaceutical Bulletin

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Microfluidics is a promising system for efficiently optimizing the experimental conditions for preparing nanomedicines, such as self-assembled nanoparticles. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles are promising drug carriers allowing sustained drug release. Here, we encapsulated the model drug curcumin, which has many pharmacological activities, into PLGA nanoparticles and investigated the effects of experimental conditions on the resulting PLGA nanoparticles using a microfluidics system with a staggered herringbone structure that can stir solutions through chaotic advection. The total flow rate and flow rate ratio of the solutions in the microfluidics system affected the diameters, polydispersity index, and encapsulation efficiency of the resulting PLGA nanoparticles and produced small, homogenous PLGA nanoparticles. The incorporation of polyethylene glycol (PEG)-PLGA into the PLGA nanoparticles reduced the particle size and improved the encapsulation efficiency. Initial burst release from the PLGA nanoparticles was prevented by the incorporation of PEG2000-PLGA. Curcumin-loaded PEGylated PLGA nanoparticles showed cytotoxicity similar to that of other formulations. This microfluidics system allows high throughput and is scalable for the efficient preparation of PLGA nanoparticles and PEGylated PLGA nanoparticles. Our results will be useful for developing novel PLGA-based polymer nanoparticles by using the microfluidics.

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“…Calcium Chloride (CaCl 2 ) is added and homogenized at 13000 rpm for 5 min. It is further stirred for 1 h 34,35 .…”

Section: Trapping Methodmentioning

confidence: 99%

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2021

IJPSR

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Nanocochleate is a novel lipid-based drug delivery system in which the liposomal vesicles are converted into nanocochleate by the addition of Calcium divalent cation. This technology involves encochleation of the drug for overcoming problems such as poor solubility, poor permeability, and poor oral bioavailability. This novel lipid-based formulation approach is applicable for the category of drugs like BCS class III drugs, BCS class IV drugs. Applications of this technology can also be extended to macromolecules as well as small molecule drugs that are lipophilic with poor oral bioavailability. It is appropriate for oral as well as systemic administration of biologically active molecules, including drugs, genes, proteins, peptides, and vaccine antigens. This article covers multiple aspects of Nanocochleate drug delivery system, such as its definition, composition, method of preparation, and mechanism of permeation. The article also elaborates on various advantages, disadvantages, and applications of Nanocochleate drug delivery system. The article highlights the potential of this novel system for oral as well as systemic delivery of chemical and biological drug products. INTRODUCTION:Successful delivery of biological molecule-based pharmaceutical products is primarily determined by its ability to cross the various barriers in the human body. Various barriers encountered by the delivery system include enzymatic barriers, intestinal epithelial barriers, capillary endothelial barriers, blood-brain barrier (BBB) 1 . The Oral route is the most common and acceptable route for drug delivery due to advantages such as high patient compliance, non-invasiveness, convenience, and acceptability. However, it is challenging to deliver biological molecules like protein and peptides, vaccines, genes, etc. by oral route due to reasons such as:

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Micro-spherical cochleate composites: method development for monodispersed cochleate system

Cited by 15 publications

References 35 publications

Dispersion and stabilization of cochleate nanoparticles

Dispersion and stabilization of cochleate nanoparticles

The Use of an Efficient Microfluidic Mixing System for Generating Stabilized Polymeric Nanoparticles for Controlled Drug Release

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